Click image to see more details
Product Info Summary
| SKU: | RP1019 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-IL17/IL17A Antibody Picoband®
View all IL-17/IL-17A Antibodies
SKU/Catalog Number
RP1019
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IL17/IL17A Antibody catalog # RP1019. Tested in ELISA, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL17/IL17A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1019)
Host
Rabbit
Contents
Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify "Carrier Free" or "No BSA" in your order note.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse IL-17 recombinant protein (Position: A26-A158).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1019 is reactive to Il17a in Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
30 kDa
Calculated molecular weight
17490 MW
Background of IL-17/IL-17A
Interleukin-17A is a protein that in humans is encoded by the IL17A gene. The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
RP1019 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA, 0.1-0.5μg/ml, -
Western blot, 0.1-0.5μg/ml, Mouse
Validation Images & Assay Conditions
Click image to see more details
Figure. Western blot analysis of IL-17 using anti-IL-17 antibody (RP1019).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane : Recombinant Mouse IL-17 Protein 0.5ng
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-17 antigen affinity purified polyclonal antibody (Catalog # RP1019) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-17 at approximately 30KD. The expected band size for IL-17 is at 30KD.
Protein Target Info & Infographic
Gene/Protein Information For Il17a (Source: Uniprot.org, NCBI)
Gene Name
Il17a
Full Name
Interleukin-17A
Weight
17490 MW
Superfamily
IL-17 family
Alternative Names
Interleukin-17A;IL-17;IL-17A;Cytotoxic T-lymphocyte-associated antigen 8;CTLA-8;Il17a;Ctla8, Il17; IL17A CTLA-8, CTLA8, IL-17, IL-17A, IL17 interleukin 17A interleukin-17A|cytotoxic T-lymphocyte-associated 8|cytotoxic T-lymphocyte-associated protein 8|interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Il17a, check out the Il17a Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Il17a: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IL17/IL17A Antibody Picoband® (RP1019)
Hello CJ!
RP1019 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Ma X, Wang L, Zhao H, Pang N, Zhang F, Jiang T, Liu X, Mamuti W, Wen H, Ding J. Mol Med Rep. 2014 Jul;10(1):236-40. Doi: 10.3892/Mmr.2014.2170. Epub 2014 Apr 24. Th17??cells Are Associated With The Th1/Th2?
Li Xn, Pan X, Qiu D. Int J Clin Exp Med. 2014 Dec 15;7(12):5324-9. Ecollection 2014. Imbalances Of Th17 And Treg Cells And Their Respective Cytokines In Copd Patients By Disease Stage.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-IL17/IL17A Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-IL17/IL17A Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
16 Customer Q&As for Anti-IL17/IL17A Antibody Picoband®
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for t-cell using anti-IL17/IL17A antibody RP1019. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-28
Question
Please see the WB image, lot number and protocol we used for t-cell using anti-IL17/IL17A antibody RP1019. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-27
Question
Our lab want to know about to test anti-IL17/IL17A antibody RP1019 on mouse t-cell for research purposes, then I may be interested in using anti-IL17/IL17A antibody RP1019 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-17
Answer
The products we sell, including anti-IL17/IL17A antibody RP1019, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-17
Question
I was wanting to use your anti-IL17/IL17A antibody for ELISA for mouse t-cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse t-cell identification?
Verified Customer
Verified customer
Asked: 2019-12-24
Answer
As indicated on the product datasheet, RP1019 anti-IL17/IL17A antibody has been validated for ELISA, WB on mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-24
Question
Is a blocking peptide available for product anti-IL17/IL17A antibody (RP1019)?
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
We do provide the blocking peptide for product anti-IL17/IL17A antibody (RP1019). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-11-28
Question
Would anti-IL17/IL17A antibody RP1019 work on monkey WB with t-cell?
Verified Customer
Verified customer
Asked: 2019-10-31
Answer
Our lab technicians have not tested anti-IL17/IL17A antibody RP1019 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-IL17/IL17A antibody RP1019 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey t-cell in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-31
Question
Will anti-IL17/IL17A antibody RP1019 work for ELISA with t-cell?
Verified Customer
Verified customer
Asked: 2019-08-01
Answer
According to the expression profile of t-cell, IL17A is highly expressed in t-cell. So, it is likely that anti-IL17/IL17A antibody RP1019 will work for ELISA with t-cell.
Boster Scientific Support
Answered: 2019-08-01
Question
Is this RP1019 anti-IL17/IL17A antibody reactive to the isotypes of IL17A?
Verified Customer
Verified customer
Asked: 2019-07-17
Answer
The immunogen of RP1019 anti-IL17/IL17A antibody is E. coli-derived mouse IL-17 recombinant protein(Position: A26-A158). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-17
Question
Can you help my question with product RP1019, anti-IL17/IL17A antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-03-05
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free RP1019 anti-IL17/IL17A antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-03-05
Question
Do you have a BSA free version of anti-IL17/IL17A antibody RP1019 available?
B. Jones
Verified customer
Asked: 2018-08-30
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-IL17/IL17A antibody RP1019 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-08-30
Question
I see that the anti-IL17/IL17A antibody RP1019 works with ELISA, what is the protocol used to produce the result images on the product page?
C. Huang
Verified customer
Asked: 2018-02-05
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-02-05
Question
We have been able to see staining in mouse t-cell. Any tips? Is anti-IL17/IL17A antibody supposed to stain t-cell positively?
Verified Customer
Verified customer
Asked: 2017-06-02
Answer
According to literature t-cell does express IL17A. According to Uniprot.org, IL17A is expressed in palpebral conjunctiva, t-cell, among other tissues. Regarding which tissues have IL17A expression, here are a few articles citing expression in various tissues:
T-cell, Pubmed ID: 7499828, 14574404
Boster Scientific Support
Answered: 2017-06-02
Question
Does RP1019 anti-IL17/IL17A antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
P. Lewis
Verified customer
Asked: 2014-11-11
Answer
As indicated on the product datasheet, RP1019 anti-IL17/IL17A antibody as been validated on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-11-11
Question
We are currently using anti-IL17/IL17A antibody RP1019 for mouse tissue, and we are content with the ELISA results. The species of reactivity given in the datasheet says mouse. Is it true that the antibody can work on dog tissues as well?
R. Jones
Verified customer
Asked: 2014-03-28
Answer
The anti-IL17/IL17A antibody (RP1019) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-03-28
Question
We bought anti-IL17/IL17A antibody for ELISA on t-cell a few years ago. I am using mouse, and I plan to use the antibody for WB next. My question regards examining t-cell as well as palpebral conjunctiva in our next experiment. Could give a recommendation on which antibody would work the best for WB?
Z. Lewis
Verified customer
Asked: 2013-06-28
Answer
I took a look at the website and datasheets of our anti-IL17/IL17A antibody and I see that RP1019 has been validated on mouse in both ELISA and WB. Thus RP1019 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2013-06-28
Question
We were happy with the WB result of your anti-IL17/IL17A antibody. However we have seen positive staining in t-cell secreted. using this antibody. Is that expected? Could you tell me where is IL17A supposed to be expressed?
S. Zhang
Verified customer
Asked: 2013-01-23
Answer
From literature, t-cell does express IL17A. Generally IL17A expresses in secreted. Regarding which tissues have IL17A expression, here are a few articles citing expression in various tissues:
T-cell, Pubmed ID: 7499828, 14574404
Boster Scientific Support
Answered: 2013-01-23
