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Product Info Summary
| SKU: | EK0431 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse IL-17/IL-17A ELISA Kit PicoKine®
View all IL-17/IL-17A ELISA kits
SKU/Catalog Number
EK0431
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse IL-17/IL-17A ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Il17a in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse IL-17/IL-17A ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0431)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: T22-A158
Sensitivity
<1 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0431 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0431 is reactive to Il17a in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0431 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IL-17/IL-17A
IL-17 is an inflammatory cytokine produced primarily by a unique lineage of CD4 T cells that plays critical roles in the pathogenesis of multiple autoimmune diseases. Interleukin-17 is expressed by activated T cells and is 57% identical to the 17- to 26-kD secretory glycoprotein encoded by gene 13 of the herpesvirus saimiri (HVS-13). IL17 induces nuclear factor kappa-B and the expression of IL6, intercellular adhesion molecule-1, granulocyte macrophage colony-stimulating factor,and prostaglandin E2, as well as the maturation of CD34 positive hematopoietic precursors into neutrophils. Anti-IL17 antibodies significantly inhibited osteoclast formation induced by culture media of RA synovial tissues. The standard product used in this kit is recombinant mouse IL-17, consisting of 137 amino acids with the molecular mass of 15.5KDa.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0431-CAP | Anti-Mouse Il17a Pre-coated 96-well strip microplate | 1 |
| EK0431-ST | Mouse Il17a Standard | 2 vials, 10 ng/tube |
| EK0431-DA | Mouse Il17a Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.030 | 0.081 | 0.161 | 0.260 | 0.495 | 0.752 | 1.221 | 1.888 |
Data Example Images
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Mouse IL-17 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect IL-17/IL-17A, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 15.6 pg/ml..
Some articles we found to cite concentrations of IL-17/IL-17A in samples: 25042924 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse IL-17/IL-17A ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 38 | 181 | 523 | 37 | 176 | 485 |
| Standard deviation | 2.35 | 10.13 | 40.27 | 2.47 | 10.91 | 41.71 |
| CV (%) | 6.2% | 5.6% | 7.7% | 6.7% | 6.2% | 8.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of IL-17/IL-17A in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 38 | 35 | 37 | 38 | 37 | 1.22 | 3.2% |
| Sample 2 | 181 | 172 | 153 | 155 | 165 | 11.71 | 7% |
| Sample 3 | 523 | 460 | 470 | 520 | 493 | 28.49 | 5.7% |
Protein Target Info & Infographic
Gene/Protein Information For Il17a (Source: Uniprot.Org, NCBI)
Gene Name
Il17a
Full Name
Interleukin-17A
Weight
17490 MW
Superfamily
IL-17 family
Alternative Names
Interleukin 17A ;Il17a ;Il17 ;mCG_7914 ; IL17A CTLA-8, CTLA8, IL-17, IL-17A, IL17 interleukin 17A interleukin-17A|cytotoxic T-lymphocyte-associated 8|cytotoxic T-lymphocyte-associated protein 8|interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Il17a, check out the Il17a Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Il17a: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse IL-17/IL-17A ELISA Kit PicoKine® (EK0431)
Hello CJ!
EK0431 has been cited in 71 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Increased expression of CXCR5 and CXCL13 in mice with experimental autoimmune myocarditis.
Dihydroartemisinin ameliorates psoriatic skin inflammation and its relapse by diminishing CD8+ T-cell memory in wild-type and humanized mice
Hybrid phage displaying SLAQVKYTSASSI induces protection against Candida albicans challenge in BALB/c mice
Alteration of T helper cell subsets in the optic nerve of experimental autoimmune encephalomyelitis
Decitabine attenuates dextran sodium sulfate‑induced ulcerative colitis through regulation of immune regulatory cells and intestinal barrier
Exclusive enteral nutrition protects against inflammatory bowel disease by inhibiting NF‑κB activation through regulation of the p38/MSK1 pathway
Identification of Toxoplasma Gondii Tyrosine Hydroxylase (TH) Activity and Molecular Immunoprotection against Toxoplasmosis
Bazedoxifene Regulates Th17 Immune Response to Ameliorate Experimental Autoimmune myocarditis via Inhibition of STAT3 Activation
Anthocyanin Protects Cardiac Function and Cardiac Fibroblasts From High-Glucose Induced Inflammation and Myocardial Fibrosis by Inhibiting IL-17
The Molecular Characterization and Immunity Identification of Trichomonas vaginalis Adhesion Protein 33 (AP33)
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Customer Reviews
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Customer Q&As
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10 Customer Q&As for Mouse IL-17/IL-17A ELISA Kit PicoKine®
Question
Is EK0431 kit guaranteed for tissue homogenates and what are the input and tips on using this kit for tissue homogenate?
Verified customer
Asked: 2019-08-16
Answer
The Mouse IL-17 ELISA Kit PicoKine EK0431 has not been validated for tissue homogenates therefore it is not guaranteed. For tissue homogenates, endogenous biotin should be considered. Endogenous biotin in tissue homogenates might introduce false positive signal. Please run the test as described below to confirm if there is Endogenous biotin in the tissue lysate samples using the ELISA kit. • Add tissue homogenates into the wells and then add ABC and TMB without adding any biotinylated detection antibody to see if any signal will be observed. • If no signal is produced, then you can work on the tissue sample by using the kit.
Boster Scientific Support
Answered: 2019-08-16
Question
Q: how can I thaw whole blood sample for IL-17 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2019-07-09
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IL-17 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2019-07-09
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2019-06-29
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-06-29
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-05-16
Question
Q: if the enzyme conjugated IL-17 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-17 antigen?
Verified Customer
Verified customer
Asked: 2017-12-19
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2017-12-19
Question
I purchased your product EK0431, how do I reconstitute my standard?
Verified Customer
Verified customer
Asked: 2017-12-14
Answer
We suggest when you reconstitute your standard, after it has been totally defrosted, spin the vial down, reconstitute with assay diluent and then spin down again before use. In making standard dilutions we recommend to keep everything on ice since diluted standard are not stable and need to be used at the same day. Mix your standard thoroughly by pipetting up and down but do not vortex the solution as this might damage the protein.
Boster Scientific Support
Answered: 2017-12-14
Question
Dear Technical Support: I am using your product EK0431 and I want to know the amino acid sequence of epitope against which primary antibody was raised used to this EK0431 kit.
Verified Customer
Verified customer
Asked: 2017-11-30
Answer
The product EK0431, unfortunately the epitope mapping has not been performed, so we do not have any information at what exact region binds to the mouse IL-17 protein. But we can let you know that this antibody pair is binding between amino acids 26 and 158 of mature mouse IL-17 as the whole mature protein used as immunogen.
Boster Scientific Support
Answered: 2017-11-30
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of IL-17 in Mouse plasma? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
S. Li
Verified customer
Asked: 2017-11-17
Answer
A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the IL-17 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2017-11-17
Question
Q: is it okay to use citrate plasma as samples in Mouse IL-17 Picokine® ELISA Kit (Catalog # EK0431)?
Verified Customer
Verified customer
Asked: 2017-06-20
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of IL-17 causing degradation of its protein structure. IL-17 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the IL-17 ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2017-06-20
Question
Q: What is the optimal O.D. value for IL-17 ELISA kit? I performed your IL-17 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain IL-17 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2016-10-18
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this IL-17 ELISA kit to have sensitivity of 1pg/ml, that means the minimum amount of IL-17 that can be declared/interpreted as positive by the above standard is 1pg/ml.
Boster Scientific Support
Answered: 2016-10-18
