Click image to see more details
Product Info Summary
| SKU: | M11385-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-IgG4 IGHG4 Rabbit Monoclonal Antibody
View all Ig gamma-4 Chain C Region Antibodies
SKU/Catalog Number
M11385-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-IgG4 IGHG4 Rabbit Monoclonal Antibody catalog # M11385-1. Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IgG4 IGHG4 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M11385-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
DCF-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human IgG4
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M11385-1 is reactive to IGHG4 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
14-36 kDa
Calculated molecular weight
35941 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M11385-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:500-1:1000
Positive Control
WB: Human spleen
IHC: human stomach carcinoma tissue
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of human IgG4 expression in Human spleen lysate.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human stomach carcinoma, using human IgG4 Antibody .
Protein Target Info & Infographic
Gene/Protein Information For IGHG4 (Source: Uniprot.org, NCBI)
Gene Name
IGHG4
Full Name
Immunoglobulin heavy constant gamma 4
Weight
35941 MW
Alternative Names
Ig gamma-4 chain C region;IGHG4; IGHG4 immunoglobulin heavy constant gamma 4 (G4m marker) immunoglobulin heavy chain constant region gamma 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IGHG4, check out the IGHG4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IGHG4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IgG4 IGHG4 Rabbit Monoclonal Antibody (M11385-1)
Hello CJ!
No publications found for M11385-1
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-IgG4 IGHG4 Rabbit Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-IgG4 IGHG4 Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
6 Customer Q&As for Anti-IgG4 IGHG4 Rabbit Monoclonal Antibody
Question
Is a blocking peptide available for product anti-IgG4 Rabbit Monoclonal antibody (M11385-1)?
Verified Customer
Verified customer
Asked: 2019-12-25
Answer
We do provide the blocking peptide for product anti-IgG4 Rabbit Monoclonal antibody (M11385-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-12-25
Question
Is there a BSA free version of anti-IgG4 Rabbit Monoclonal antibody M11385-1 available?
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IgG4 Rabbit Monoclonal antibody M11385-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-11
Question
Can you help my question with product M11385-1, anti-IgG4 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M11385-1 anti-IgG4 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-09
Question
We need to test anti-IgG4 Rabbit Monoclonal antibody M11385-1 on human lymph node for research purposes, then I may be interested in using anti-IgG4 Rabbit Monoclonal antibody M11385-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-03-28
Answer
The products we sell, including anti-IgG4 Rabbit Monoclonal antibody M11385-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-03-28
Question
Does M11385-1 anti-IgG4 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-11-19
Answer
As indicated on the product datasheet, M11385-1 anti-IgG4 Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-11-19
Question
Will anti-IgG4 Rabbit Monoclonal antibody M11385-1 work on monkey WB with liver?
Verified Customer
Verified customer
Asked: 2018-01-24
Answer
Our lab technicians have not validated anti-IgG4 Rabbit Monoclonal antibody M11385-1 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-IgG4 Rabbit Monoclonal antibody M11385-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-24
