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Product Info Summary
| SKU: | A05134 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-UBF1 UBTF Antibody
SKU/Catalog Number
A05134
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-UBF1 UBTF Antibody (Catalog # A05134). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-UBF1 UBTF Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05134)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human UBF1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05134 is reactive to UBTF in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
111 kDa
Calculated molecular weight
89406 MW
Background of UBTF
Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element.
Jantzen H.M., Nature 344:830-836(1990).
Chan E.K.L., J. Exp. Med. 174:1239-1244(1991).
Molina H., Proc. Natl. Acad. Sci. U.S.A. 104:2199-2204(2007).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05134 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
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Western blot analysis of extracts from HeLa cells and Jurkat cells, using UBF1 antibody A05134.
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Immunohistochemistry analysis of paraffin-embedded human brain tissue using UBF1 antibody A05134.
Protein Target Info & Infographic
Gene/Protein Information For UBTF (Source: Uniprot.org, NCBI)
Gene Name
UBTF
Full Name
Nucleolar transcription factor 1
Weight
89406 MW
Alternative Names
Nucleolar transcription factor 1;Autoantigen NOR-90;Upstream-binding factor 1;UBF-1;UBTF;UBF, UBF1; UBTF CONDBA, NOR-90, UBF, UBF-1, UBF1, UBF2 upstream binding transcription factor nucleolar transcription factor 1|90-kDa nucleolus organizer region auto|auto NOR-90|upstream binding transcription factor, RNA polymerase I
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on UBTF, check out the UBTF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for UBTF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-UBF1 UBTF Antibody (A05134)
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Customer Q&As
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3 Customer Q&As for Anti-UBF1 UBTF Antibody
Question
We are currently using anti-UBF1 antibody A05134 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-18
Answer
The anti-UBF1 antibody (A05134) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-18
Question
I was wanting to use your anti-UBF1 antibody for WB for human cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cervix carcinoma erythroleukemia identification?
B. Roberts
Verified customer
Asked: 2019-12-12
Answer
As indicated on the product datasheet, A05134 anti-UBF1 antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-12
Question
Is this A05134 anti-UBF1 antibody reactive to the isotypes of UBTF?
Verified Customer
Verified customer
Asked: 2019-05-30
Answer
The immunogen of A05134 anti-UBF1 antibody is Synthesized peptide derived from internal of human UBF1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-30
