Anti-IgG4 (Ig Heavy Constant Gamma 4) (G4m Marker) Monoclonal Antibody

Product Name

Anti-IgG4 (Ig Heavy Constant Gamma 4) (G4m Marker) Monoclonal Antibody

View all Ig gamma-4 Chain C Region Antibodies

SKU/Catalog Number

M11385

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-IgG4 (Ig Heavy Constant Gamma 4) (G4m Marker) Monoclonal Antibody (Catalog # M11385). Tested in IHC applications. This antibody reacts with Human.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-IgG4 (Ig Heavy Constant Gamma 4) (G4m Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M11385)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: IGHG4/1345

Isotype

IgG1, kappa

Immunogen

Recombinant human IGHG4 fragment

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M11385 is reactive to IGHG4 in Human

Applications

M11385 is guaranteed for IHC Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

35941 MW

Background of Ig gamma-4 Chain C Region

The regions of relatively constant sequence beyond the variable regions of immunoglobulins are termed constant regions (C regions) and are present in both the heavy and light chains. With very few exceptions, the sites of attachment for carbohydrates on immunoglobulins are located in these C regions. These regions also function to hold the variable regions together by using the disulfide bond between them. The C regions facilitate interaction with the antigen by increasing the maximum rotation of the immunoglobulin arms. Reportedly, a large population of patients with recurrent respiratory tract infection has low IgG4 concentrations. IgG4-related sclerosing disease has been recognized as a systemic disease entity characterized by an elevated serum IgG4 level, sclerosing fibrosis, and diffuse lymphoplasmacytic infiltration with the presence of many IgG4-positive plasma cells.IgG4 is overexpressed in inflammatory pseudotumor (IPT) and under expressed in inflammatory myofibroblastic tumor (IMT). In pulmonary nodular lymphoid hyperplasia (PNLH), there are an increased number of IgG4+ plasma cells.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

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Formalin-fixed, paraffin-embedded human Tonsil stained with Anti-IgG4 Mouse Monoclonal Antibody (IGHG4/1345).

Gene/Protein Information For IGHG4 (Source: Uniprot.org, NCBI)

Alternative Names

IGHG4 ; mmunoglobulin heavy constant gamma 4 (G4m marker); mmunoglobulin heavy constant gamma 4 IGHG4 immunoglobulin heavy constant gamma 4 (G4m marker) immunoglobulin heavy chain constant region gamma 4

For more info on IGHG4, check out the IGHG4 Infographic

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In this infographic, you will see the following information for IGHG4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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