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Product Info Summary
| SKU: | M11385-3 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA |
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Product info
Product Name
Anti-Human IgG4 Fc IGHG4 Rabbit Monoclonal Antibody, Clone#RM217
View all Ig gamma-4 Chain C Region Antibodies
SKU/Catalog Number
M11385-3
Size
100ug
Form
Liquid
Description
Boster Bio Anti-Human IgG4 Fc IGHG4 Rabbit Monoclonal Antibody, Clone#RM217 (Catalog # M11385-3). Tested in ELISA applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Human IgG4 Fc IGHG4 Rabbit Monoclonal Antibody, Clone#RM217 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M11385-3)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM217
Isotype
Rabbit IgG
Immunogen
Peptide corresponding to the Fc region of Human IgG4
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to the Fc region of human IgG4. No cross-reactivity with human IgG1, IgG2, IgG3, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.
Reactive Species
M11385-3 is reactive to IGHG4 in Human
Reconstitution
Calculated molecular weight
35941 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M11385-3 is guaranteed for ELISA Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 50ng/well – 200ng/well (for Capture)
0.05ug/mL – 0.2ug/mL (for Detection).
Validation Images & Assay Conditions
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Figure 1. Specificity Test result
RM217 specifically reacts to human IgG4. No cross reactivity with human IgG1, IgG2, IgG3
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Figure 2. ELISA result showing specificity
Sandwich ELISA using RM217 as the capture antibody, and Biotinylated anti-human light chains (+) antibody RM129 as the detection antibody, followed by an AP conjugated streptavidin.
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Figure 3. ELISA result showing specificity
Sandwich ELISA using RM217 as the capture antibody, and Biotinylated anti-human light chains (+) antibody RM129 as the detection antibody, followed by an AP conjugated streptavidin.
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Figure 4. ELISA result showing specificity
A titer ELISA using RM217. The plate was coated with different amounts of human IgG4. A serial dilution of RM217 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Protein Target Info & Infographic
Gene/Protein Information For IGHG4 (Source: Uniprot.org, NCBI)
Gene Name
IGHG4
Full Name
Immunoglobulin heavy constant gamma 4
Weight
35941 MW
Alternative Names
Immunoglobulin heavy constant gamma 4 ;Ig gamma-4 chain C region ;IGHG4 ; IGHG4 immunoglobulin heavy constant gamma 4 (G4m marker) immunoglobulin heavy chain constant region gamma 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IGHG4, check out the IGHG4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IGHG4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human IgG4 Fc IGHG4 Rabbit Monoclonal Antibody, Clone#RM217 (M11385-3)
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Customer Q&As
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1 Customer Q&As for Anti-Human IgG4 Fc IGHG4 Rabbit Monoclonal Antibody, Clone#RM217
Question
We are currently using anti-Human IgG4 Fc Rabbit Monoclonal antibody, Clone#RM217 M11385-3 for human tissue, and we are satisfied with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2018-11-05
Answer
The anti-Human IgG4 Fc Rabbit Monoclonal antibody, Clone#RM217 (M11385-3) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-11-05
