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Product Info Summary
| SKU: | M11385-2 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IHC, ICC |
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Product info
Product Name
Anti-Human IgG4 IGHG4 Rabbit Monoclonal Antibody, Clone#RM120
View all Ig gamma-4 Chain C Region Antibodies
SKU/Catalog Number
M11385-2
Size
100ug
Form
Liquid
Description
Boster Bio Anti-Human IgG4 IGHG4 Rabbit Monoclonal Antibody, Clone#RM120 (Catalog # M11385-2). Tested in ELISA, ICC, IHC, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Human IgG4 IGHG4 Rabbit Monoclonal Antibody, Clone#RM120 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M11385-2)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM120
Isotype
Rabbit IgG
Immunogen
Peptide corresponding to the hinge region of Human IgG4
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Reacts to the heavy chain of human IgG4, RM120 does not cross-react to any other IgG subclasses (IgG1, IgG2, IgG3), and shows no cross-reactivity to IgM, IgA, IgD, IgE. RM120 does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.
Reactive Species
M11385-2 is reactive to IGHG4 in Human
Reconstitution
Calculated molecular weight
35941 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M11385-2 is guaranteed for ELISA, Flow Cytometry, IHC, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 50ng/well – 200ng/well (for Capture)
0.05ug/mL – 0.2ug/mL (for Detection)
WB: 0.5 ug/mL – 2 ug/mL
Flow Cytometry (FC): 1ug/mL-4ug/mL.
Validation Images & Assay Conditions
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Figure 1. Western Blotting result
Western Blot of human, mouse, rat, and goat IgG shows RM120 reacted to human IgG4 both whole molecule (~150kDa, non-reduced) and heavy chain (~50kDa, reduced). No cross reactivity with other isotype of human IgG, and mouse, rat, or goat IgG.
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Figure 2. IHC result
Immunohistochemistry of human lymphoid tissue using anti-human IgG4 antibody RM120.
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Figure 3. ELISA result showing specificity
Detection of human IgG4 in monkey serum. Sandwich ELISA using RM120 as the capture antibody, and biotinylated anti-human light chains ( or ) antibody RM129 as the detection antibody, followed by a HRP conjugated streptavidin.
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Figure 4. ELISA result showing specificity
Sandwich ELISA using RM120 as the capture antibody, and Biotinylated anti-human light chains ( + ) antibody RM129 as the detection antibody, followed by an AP conjugated streptavidin.
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Figure 5. ELISA result showing specificity
A titer ELISA using RM120. The plate was coated with different amounts of human IgG4. A serial dilution of RM120 was used as the primary antibody, followed by an alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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Figure 6. ELISA result showing specificity
ELISA showing RM120 does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of RM120 and a monkey IgG binding antibody (positive control) was used as the detection antibody.
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Figure 7. IHC result
Immunohistochemical staining of FFPE Tonsil tissue sections using Anti-Human IgG4 antibody RM120 at a 1:50,000 dilution.
Protein Target Info & Infographic
Gene/Protein Information For IGHG4 (Source: Uniprot.org, NCBI)
Gene Name
IGHG4
Full Name
Immunoglobulin heavy constant gamma 4
Weight
35941 MW
Alternative Names
Immunoglobulin heavy constant gamma 4 ;Ig gamma-4 chain C region ;IGHG4 ; IGHG4 immunoglobulin heavy constant gamma 4 (G4m marker) immunoglobulin heavy chain constant region gamma 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IGHG4, check out the IGHG4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IGHG4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human IgG4 IGHG4 Rabbit Monoclonal Antibody, Clone#RM120 (M11385-2)
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