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Product Info Summary
| SKU: | A00306 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB, IHC-P-Leica |
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Product info
Product Name
Anti-IFNAR1 Antibody (Center)
View all IFN-alpha/beta R1 Antibodies
SKU/Catalog Number
A00306
Size
80 µl
Form
Liquid
Description
Boster Bio Anti-IFNAR1 Antibody (Center) (Catalog # A00306). Tested in WB, Flow Cytometry, IHC-P-Leica application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-IFNAR1 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00306)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This IFNAR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 162-188 amino acids of human IFNAR1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00306 is reactive to IFNAR1 in Human
Reconstitution
Calculated molecular weight
63525 Da
Background of IFN-alpha/beta R1
IFNAR1 is the receptor for interferons alpha and beta. Binding to type I IFNs triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT proteins and IFNR alpha-and beta-subunits themselves.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00306 is guaranteed for Flow Cytometry, WB, IHC-P-Leica Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P-Leica: 1:1000
FC: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-IFNAR1 Antibody (Center) at 1:1000 dilution
Lane 1: Hela whole cell lysate
Lane 2: 293 whole cell lysate
Lane 3: Jurkat whole cell lysate
Lane 4: H. brain whole cell lysate
Lane 5: K562 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 64 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-IFNAR1 Antibody (Center) at 1:1000 dilution
Lane 1: Hela whole cell lysate
Lane 2: 293T whole cell lysate
Lane 3: Jurkat whole cell lysate
Lane 4: human brain lysate
Lane 5: human heart lysate
Lane 6: K562 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 64 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded human brain tissue using A00306 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature; antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody (1:1000) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
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Overlay histogram showing K562 cells stained with A00306 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (A00306, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Protein Target Info & Infographic
Gene/Protein Information For IFNAR1 (Source: Uniprot.org, NCBI)
Gene Name
IFNAR1
Full Name
Interferon alpha/beta receptor 1
Weight
63525 Da
Superfamily
type II cytokine receptor family
Alternative Names
Interferon alpha/beta receptor 1, IFN-R-1, IFN-alpha/beta receptor 1, Cytokine receptor class-II member 1, Cytokine receptor family 2 member 1, CRF2-1, Type I interferon receptor 1, IFNAR1, IFNAR IFNAR1 AVP, IFN-alpha-REC, IFNAR, IFNBR, IFRC interferon alpha and beta receptor subunit 1 interferon alpha/beta receptor 1|CRF2-1|IFN-R-1|IFN-alpha/beta receptor 1|IFNalpha/beta receptor 1|alpha-type antiviral protein|beta-type antiviral protein|cytokine receptor class-II member 1|cytokine receptor family 2 member 1|interferon (alpha, beta and omega) receptor 1|interferon receptor 1|interferon-alpha/beta receptor alpha chain|interferon-beta receptor 1|type I interferon receptor 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IFNAR1, check out the IFNAR1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IFNAR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IFNAR1 Antibody (Center) (A00306)
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