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Product Info Summary
| SKU: | A00424 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-TRAF3 Antibody Picoband®
SKU/Catalog Number
A00424
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TRAF3 Antibody Picoband® catalog # A00424. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TRAF3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00424)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human TRAF3 recombinant protein (Position: F298-Q551).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00424 is reactive to TRAF3 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
60 kDa, 64 kDa
Calculated molecular weight
64.49kDa
Background of TRAF-3
Tetraspanin-12 (Tspan-12) also known as tetraspan NET-2 (NET2) or transmembrane 4 superfamily member 12 (TM4SF12) is a tetraspanin protein that in humans is encoded by the TSPAN12 gene. The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00424 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human K562 cell, rat brain tissue, rat PC-12 cell, mouse brain tissue, mouse HEPA1-6 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of TRAF3 using anti-TRAF3 antibody (A00424).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human K562 cell lysates,
Lane 2: rat brain tissue lysates,
Lane 3: rat PC-12 cell lysates,
Lane 4: mouse brain tissue lysates,
Lane 5: mouse HEPA1-6 cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAF3 antigen affinity purified polyclonal antibody (Catalog # A00424) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAF3 at approximately 60,64KD. The expected band size for TRAF3 is at 64KD.
Protein Target Info & Infographic
Gene/Protein Information For TRAF3 (Source: Uniprot.org, NCBI)
Gene Name
TRAF3
Full Name
TNF receptor-associated factor 3
Weight
64.49kDa
Superfamily
TNF receptor-associated factor family
Alternative Names
TNF receptor-associated factor 3; CAP-1; CD40 receptor-associated factor 1; CRAF1; CD40-binding protein; CD40BP; LMP1-associated protein 1; LAP1; RING-type E3 ubiquitin transferase TRAF3 ; TRAF3; CAP1; CRAF1 TRAF3 CAP-1, CAP1, CD40bp, CRAF1, IIAE5, LAP1, RNF118 TNF receptor associated factor 3 TNF receptor-associated factor 3|CD40 associated protein 1|CD40 binding protein|CD40 receptor associated factor 1|LMP1-associated protein 1|RING-type E3 ubiquitin transferase TRAF3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TRAF3, check out the TRAF3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TRAF3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TRAF3 Antibody Picoband® (A00424)
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