Product Info Summary
SKU: | A01281-Dyl488 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | Flow Cytometry |
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Product info
Product Name
Anti-Human TAPA1 DyLight® 488 conjugated CD81 Antibody
SKU/Catalog Number
A01281-Dyl488
Size
100 μg/vial
Form
Liquid
Description
Boster Bio Anti-Human TAPA1 DyLight® 488 conjugated CD81 Antibody catalog # A01281-Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Cite This Product
Anti-Human TAPA1 DyLight® 488 conjugated CD81 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01281-Dyl488)
Host
Rabbit
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human TAPA1 recombinant protein (Position: F113-K201).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01281-Dyl488 is reactive to CD81 in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
25.809kDa
Background of CD81
CD81 molecule, also known as CD81 (Cluster of Differentiation 81), is a protein which in humans is encoded by the CD81 gene. The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. Two transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01281-Dyl488 is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (Fixed), 1-3μg/1x106 cells
Validation Images & Assay Conditions
Click image to see more details
Boster Kit Box
Protein Target Info & Infographic
Gene/Protein Information For CD81 (Source: Uniprot.org, NCBI)
Gene Name
CD81
Full Name
CD81 antigen
Weight
25.809kDa
Superfamily
tetraspanin (TM4SF) family
Alternative Names
CD81 antigen; 26 kDa cell surface protein TAPA-1; Target of the antiproliferative antibody 1; Tetraspanin-28; Tspan-28; CD81; CD81; TAPA1; TSPAN28 CD81 CVID6, S5.7, TAPA1, TSPAN28 CD81 molecule CD81 |26 kDa cell surface protein TAPA-1|CD81 (target of antiproliferative 1)|tetraspanin-28|tspan-28
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD81, check out the CD81 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD81: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human TAPA1 DyLight® 488 conjugated CD81 Antibody (A01281-Dyl488)
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-Human TAPA1 DyLight® 488 conjugated CD81 Antibody
Question
We are currently using anti-Human TAPA1 DyLight® 488 conjugated antibody A01281-Dyl488 for human tissue, and we are satisfied with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-09
Answer
The anti-Human TAPA1 DyLight® 488 conjugated antibody (A01281-Dyl488) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-09
Question
We have been able to see staining in human myometrium. Are there any suggestions? Is anti-Human TAPA1 DyLight® 488 conjugated antibody supposed to stain myometrium positively?
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
From what I have seen in literature myometrium does express CD81. From what I have seen in Uniprot.org, CD81 is expressed in myometrium, brain, among other tissues. Regarding which tissues have CD81 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-01-03
Question
Is this A01281-Dyl488 anti-Human TAPA1 DyLight® 488 conjugated antibody reactive to the isotypes of CD81?
Verified Customer
Verified customer
Asked: 2019-11-25
Answer
The immunogen of A01281-Dyl488 anti-Human TAPA1 DyLight® 488 conjugated antibody is E. coli-derived human TAPA1 recombinant protein (Position: F113-K201). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-25
Question
We were satisfied with the WB result of your anti-Human TAPA1 DyLight® 488 conjugated antibody. However we have been able to see positive staining in brain cell membrane using this antibody. Is that expected? Could you tell me where is CD81 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-01-25
Answer
From literature, brain does express CD81. Generally CD81 expresses in cell membrane. Regarding which tissues have CD81 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-01-25
Question
We need to test anti-Human TAPA1 DyLight® 488 conjugated antibody A01281-Dyl488 on human myometrium for research purposes, then I may be interested in using anti-Human TAPA1 DyLight® 488 conjugated antibody A01281-Dyl488 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
L. Moore
Verified customer
Asked: 2018-03-13
Answer
The products we sell, including anti-Human TAPA1 DyLight® 488 conjugated antibody A01281-Dyl488, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-03-13
Question
I was wanting to use your anti-Human TAPA1 DyLight® 488 conjugated antibody for Flow Cytometry for human myometrium on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human myometrium identification?
J. Krishna
Verified customer
Asked: 2017-08-25
Answer
As indicated on the product datasheet, A01281-Dyl488 anti-Human TAPA1 DyLight® 488 conjugated antibody has been validated for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human myometrium in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-08-25