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Product Info Summary
| SKU: | M04575-5 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IHC, ICC |
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Product info
Product Name
Anti-Human IgG1 IGHG1 Rabbit Monoclonal Antibody, Clone#RM117
SKU/Catalog Number
M04575-5
Size
100ug
Form
Liquid
Description
Boster Bio Anti-Human IgG1 IGHG1 Rabbit Monoclonal Antibody, Clone#RM117 (Catalog # M04575-5). Tested in ELISA, ICC, IHC, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Human IgG1 IGHG1 Rabbit Monoclonal Antibody, Clone#RM117 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04575-5)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM117
Isotype
Rabbit IgG
Immunogen
Peptide corresponding to the hinge region of Human IgG1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Reacts to the heavy chain of human IgG1. RM117 does not cross-react to any other IgG subclasses (IgG2, IgG3, IgG4), and shows no cross-reactivity to IgM, IgA, IgD, IgE. RM117 does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.
Reactive Species
M04575-5 is reactive to IGHG1 in Human
Reconstitution
Calculated molecular weight
36106 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04575-5 is guaranteed for ELISA, Flow Cytometry, IHC, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 50ng/well – 200ng/well (for Capture)
0.05ug/mL – 0.2ug/mL (for Detection)
Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL
Immunohistochemistry (IHC): 0.5ug/mL-2ug/mL
Flow Cytometry (FC): 2ug/mL-8ug/mL.
Validation Images & Assay Conditions
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Figure 1. ELISA result showing specificity
Detection of human IgG1 in monkey serum, using RM117 (capture) and biotin-RM129 (detection) as a Sandwich ELISA pair. HRP conjugated streptavidin and TMB were used to yield the colorimetric reaction.
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Figure 2. ELISA result showing specificity
Sandwich ELISA using RM117 as the capture antibody, and Biotinylated anti-human light chains (k+) antibody RM129 as the detection antibody, followed by an AP conjugated streptavidin
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Figure 3. ELISA result showing specificity
ELISA of human immunoglobulins shows RM117 only reacted to human IgG1. No cross reactivity with Human IgG2, IgG3, IgG4, IgE, IgD, IgA, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM117 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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Figure 4. ELISA result showing specificity
A titer ELISA using RM117. The plate was coated with different amounts of human IgG1. A serial dilution of RM117 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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Figure 5. ELISA result showing specificity
ELISA showing RM117 does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of RM117 and a monkey IgG binding antibody (positive control) was used as the detection antibody.
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Figure 6. IHC result
Immunohistochemical staining of FFPE Tonsil tissue sections using Anti-Human IgG1 antibody RM117 at a 1:15,000 dilution.
Protein Target Info & Infographic
Gene/Protein Information For IGHG1 (Source: Uniprot.org, NCBI)
Gene Name
IGHG1
Full Name
Immunoglobulin heavy constant gamma 1
Weight
36106 MW
Alternative Names
Immunoglobulin heavy constant gamma 1 ;Ig gamma-1 chain C region ;Ig gamma-1 chain C region EU ;Ig gamma-1 chain C region KOL ;Ig gamma-1 chain C region NIE ;IGHG1 ; IGHG1 immunoglobulin heavy constant gamma 1 (G1m marker) constant region of heavy chain of IgG1|immunoglobulin gamma 1 (Gm marker)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IGHG1, check out the IGHG1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IGHG1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human IgG1 IGHG1 Rabbit Monoclonal Antibody, Clone#RM117 (M04575-5)
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