Anti-HARS/Hisrs Antibody

Product Name

Anti-HARS/Hisrs Antibody

View all HARS1 Antibodies

SKU/Catalog Number

A05242

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-HARS/Hisrs Antibody (Catalog # A05242). Tested in WB, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HARS/Hisrs Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05242)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from C-terminal of human HARS.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A05242 is reactive to HARS in Human, Mouse

Reconstitution

Observed Molecular Weight

111 kDa

Calculated molecular weight

57411 MW

Background of HARS1

Defects in HARS are a cause of Usher syndrome type 3B (USH3B). USH3B is a syndrome characterized by progressive vision and hearing loss during early childhood. Some patients have the so-called 'Charles Bonnet syndrome,' involving decreased visual acuity and vivid visual hallucinations. USH is a genetically heterogeneous condition characterized by the association of retinitis pigmentosa with sensorineural deafness. Age at onset and differences in auditory and vestibular function distinguish Usher syndrome type 1 (USH1), Usher syndrome type 2 (USH2) and Usher syndrome type 3 (USH3). USH3 is characterized by postlingual, progressive hearing loss, variable vestibular dysfunction, and onset of retinitis pigmentosa symptoms, including nyctalopia, constriction of the visual fields, and loss of central visual acuity, usually by the second decade of life. Belongs to the class-II aminoacyl-tRNA synthetase family.

Raben N., Nucleic Acids Res. 20:1075-1081(1992).
Tsui F.W.L., Nucleic Acids Res. 15:3349-3367(1987).
Tsui H.W., Gene 131:201-208(1993).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A05242 is guaranteed for IF, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Immunofluorescence: 1:100~1:500

Validation Images & Assay Conditions

Gene/Protein Information For HARS (Source: Uniprot.org, NCBI)

Alternative Names

Histidine--tRNA ligase, cytoplasmic;6.1.1.21;Histidyl-tRNA synthetase;HisRS;HARS;HRS; HARS1 CMT2W, HARS, HRS, USH3B histidyl-tRNA synthetase 1 histidine--tRNA ligase, cytoplasmic|HisRS|Jo-1 |histidine translase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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