Click image to see more details
Product Info Summary
| SKU: | M30946 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC-P |
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Product info
Product Name
Anti-IgG (Immunoglobulin Gamma Heavy Chain) (B-Cell Marker) Monoclonal Antibody
SKU/Catalog Number
M30946
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-IgG (Immunoglobulin Gamma Heavy Chain) (B-Cell Marker) Monoclonal Antibody (Catalog # M30946). Tested in Flow Cytometry, IF, IHC-P applications. This antibody reacts with Human.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-IgG (Immunoglobulin Gamma Heavy Chain) (B-Cell Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M30946)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: B33/20
Isotype
IgG1, kappa
Immunogen
Purified polyclonal human Ig Gamma Chain
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M30946 is reactive to Ighg in Human
Applications
M30946 is guaranteed for Flow Cytometry, IF, IHC-P Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
36106 MW
Background of Ighg
Recognizes a protein of 75kDa, identified as gamma heavy chain of human immunoglobulins. Its epitope maps in CH2 domain of Fc region of IgG. It reacts with all sub-classes of gamma chain of human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This monoclonal antibody is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Reconstitute with distilled water.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin/paraffin) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
Click image to see more details
Formalin-fixed, paraffin-embedded human Tonsil stained with Anti-IgG Monoclonal Antibody (B33/20)
Protein Target Info & Infographic
Gene/Protein Information For Ighg (Source: Uniprot.org, NCBI)
Gene Name
Ighg
Full Name
Ig gamma-2A chain C region, A allele
Weight
36106 MW
Alternative Names
Ig gamma-2A chain C region, A allele Ighg|AU044919, IGHG2A|Immunoglobulin heavy chain (gamma polypeptide)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Ighg, check out the Ighg Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Ighg: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IgG (Immunoglobulin Gamma Heavy Chain) (B-Cell Marker) Monoclonal Antibody (M30946)
Hello CJ!
M30946 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Li B,Xiang Z,Xiong F,Yan B,Huang Q.C-terminal binding protein-2 is a prognostic marker for lung adenocarcinomas.Medicine (Baltimore).2020 Jul 31;99(31):e20076.doi: 10.1097/MD.0000000000020076.PMID:32756072;PMCID:PMC7402787.
Species: Human
M30946 usage in article: APP:IHC, SAMPLE:NSCLC TISSUE, DILUTION:1:400
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Customer Q&As
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7 Customer Q&As for Anti-IgG (Immunoglobulin Gamma Heavy Chain) (B-Cell Marker) Monoclonal Antibody
Question
Will anti-IgG (Immunoglobulin Gamma Heavy Chain) (B-Cell Marker) Monoclonal antibody M30946 work for IHC with lymph node?
Verified Customer
Verified customer
Asked: 2020-03-16
Answer
According to the expression profile of lymph node, IGHG1 is highly expressed in lymph node. So, it is likely that anti-IgG (Immunoglobulin Gamma Heavy Chain) (B-Cell Marker) Monoclonal antibody M30946 will work for IHC with lymph node.
Boster Scientific Support
Answered: 2020-03-16
Question
My question regarding product M30946, anti-IgG (Immunoglobulin Gamma Heavy Chain) (B-Cell Marker) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-25
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M30946 anti-IgG (Immunoglobulin Gamma Heavy Chain) (B-Cell Marker) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-25
Question
We are currently using anti-IgG (Immunoglobulin Gamma Heavy Chain) (B-Cell Marker) Monoclonal antibody M30946 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-24
Answer
The anti-IgG (Immunoglobulin Gamma Heavy Chain) (B-Cell Marker) Monoclonal antibody (M30946) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-24
Question
I see that the anti-IgG (Immunoglobulin Gamma Heavy Chain) (B-Cell Marker) Monoclonal antibody M30946 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-03
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-03
Question
Do you have a BSA free version of anti-IgG (Immunoglobulin Gamma Heavy Chain) (B-Cell Marker) Monoclonal antibody M30946 available?
Verified Customer
Verified customer
Asked: 2018-02-21
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-IgG (Immunoglobulin Gamma Heavy Chain) (B-Cell Marker) Monoclonal antibody M30946 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-02-21
Question
Does anti-IgG (Immunoglobulin Gamma Heavy Chain) (B-Cell Marker) Monoclonal antibody M30946 work on horse IHC with liver?
Verified Customer
Verified customer
Asked: 2017-10-05
Answer
Our lab technicians have not validated anti-IgG (Immunoglobulin Gamma Heavy Chain) (B-Cell Marker) Monoclonal antibody M30946 on horse. You can run a BLAST between horse and the immunogen sequence of anti-IgG (Immunoglobulin Gamma Heavy Chain) (B-Cell Marker) Monoclonal antibody M30946 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse liver in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-10-05
Question
I have attached the WB image, lot number and protocol we used for lymph node using anti-IgG (Immunoglobulin Gamma Heavy Chain) (B-Cell Marker) Monoclonal antibody M30946. Please let me know if you require anything else.
P. Krishna
Verified customer
Asked: 2016-12-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-12-21
