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Product Info Summary
| SKU: | M04575-2 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Mouse IgG1 Rabbit Monoclonal Antibody, Clone#RM106
SKU/Catalog Number
M04575-2
Size
100ug
Form
Liquid
Description
Boster Bio Anti-Mouse IgG1 Rabbit Monoclonal Antibody, Clone#RM106 (Catalog # M04575-2). Tested in ELISA, WB applications. This antibody reacts with Mouse.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Mouse IgG1 Rabbit Monoclonal Antibody, Clone#RM106 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04575-2)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM106
Isotype
Rabbit IgG
Immunogen
Mouse IgG
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to the Fc region of mouse IgG1. No cross-reactivity with mouse IgG2a, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. It may slightly cross-react to mouse IgG2b, and may also react to goat IgG.
Reactive Species
M04575-2 is reactive to Ighg1 in Mouse
Reconstitution
Calculated molecular weight
35705 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04575-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 0.005 ug/mL-0.2 ug/mL
WB: 0.1 ug/mL-0.5 ug/mL.
Validation Images & Assay Conditions
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Western blot of nonreduced(-) and reduced(+) mouse IgG1 (20ng/lane), using 0.2ug/mL of clone RM106. This antibody reacts to nonreduced IgG1 (~150 kDa) stronger than the reduced γ1 form (~50 kDa).
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ELISA of mouse immunoglobulins shows RM106 reacts to the Fc region of mouse IgG1; no cross reactivity with IgG2a, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM106 was used as the primary antibody. An alkaline phosphatase conjugated anti- rabbit IgG as the secondary antibody.
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ELISA of mouse immunoglobulins shows RM106 reacts to the Fc region of mouse IgG1; no cross reactivity with IgG2a, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM106 was used as the primary antibody. An alkaline phosphatase conjugated anti- rabbit IgG as the secondary antibody.
Protein Target Info & Infographic
Gene/Protein Information For Ighg1 (Source: Uniprot.org, NCBI)
Gene Name
Ighg1
Full Name
Immunoglobulin heavy constant gamma 1
Weight
35705 MW
Alternative Names
Ig gamma-1 chain C region secreted form;Ighg1;Igh-4; IGHG1 immunoglobulin heavy constant gamma 1 (G1m marker) constant region of heavy chain of IgG1|immunoglobulin gamma 1 (Gm marker)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Ighg1, check out the Ighg1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Ighg1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Mouse IgG1 Rabbit Monoclonal Antibody, Clone#RM106 (M04575-2)
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