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Product Info Summary
| SKU: | A04131 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-SKIL/Snon Antibody
SKU/Catalog Number
A04131
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-SKIL/Snon Antibody (Catalog # A04131). Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SKIL/Snon Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04131)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from C-terminal of human SKIL.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04131 is reactive to SKIL in Human, Mouse
Reconstitution
Calculated molecular weight
76976 MW
Background of SnoN
May have regulatory role in cell division or differentiation in response to extracellular signals.
Nomura N., Nucleic Acids Res. 17:5489-5500(1989).
Pearson-White S.H., Nucleic Acids Res. 25:2930-2937(1997).
Pearson-White S., Nucleic Acids Res. 21:4632-4638(1993).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04131 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from HUVEC cells and Jurkat cells, using SKIL antibody A04131.
Protein Target Info & Infographic
Gene/Protein Information For SKIL (Source: Uniprot.org, NCBI)
Gene Name
SKIL
Full Name
Ski-like protein
Weight
76976 MW
Superfamily
SKI family
Alternative Names
Ski-like protein;Ski-related oncogene;Ski-related protein;SKIL;SNO; SKIL SNO, SnoA, SnoI, SnoN SKI like proto-oncogene ski-like protein|SKI-like oncogene|ski-related oncogene snoN
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SKIL, check out the SKIL Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SKIL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SKIL/Snon Antibody (A04131)
Hello CJ!
No publications found for A04131
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Customer Q&As
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13 Customer Q&As for Anti-SKIL/Snon Antibody
Question
We are currently using anti-SKIL/Snon antibody A04131 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on canine tissues as well?
A. Collins
Verified customer
Asked: 2020-03-12
Answer
The anti-SKIL/Snon antibody (A04131) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-12
Question
Will A04131 anti-SKIL/Snon antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-15
Answer
It shows on the product datasheet, A04131 anti-SKIL/Snon antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-15
Question
Is this A04131 anti-SKIL/Snon antibody reactive to the isotypes of SKIL?
Verified Customer
Verified customer
Asked: 2019-10-24
Answer
The immunogen of A04131 anti-SKIL/Snon antibody is Synthesized peptide derived from C-terminal of human SKIL. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-24
Question
Is a blocking peptide available for product anti-SKIL/Snon antibody (A04131)?
Verified Customer
Verified customer
Asked: 2019-07-26
Answer
We do provide the blocking peptide for product anti-SKIL/Snon antibody (A04131). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-07-26
Question
Is there a BSA free version of anti-SKIL/Snon antibody A04131 available?
Verified Customer
Verified customer
Asked: 2019-05-10
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-SKIL/Snon antibody A04131 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-10
Question
We want to test anti-SKIL/Snon antibody A04131 on mouse tendon of biceps brachii for research purposes, then I may be interested in using anti-SKIL/Snon antibody A04131 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
M. Miller
Verified customer
Asked: 2019-03-12
Answer
The products we sell, including anti-SKIL/Snon antibody A04131, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-03-12
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for tendon of biceps brachii using anti-SKIL/Snon antibody A04131. Let me know if you need anything else.
T. Miller
Verified customer
Asked: 2018-12-17
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-12-17
Question
I was wanting to use your anti-SKIL/Snon antibody for WB for mouse tendon of biceps brachii on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse tendon of biceps brachii identification?
Verified Customer
Verified customer
Asked: 2018-01-22
Answer
You can see on the product datasheet, A04131 anti-SKIL/Snon antibody has been tested for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse tendon of biceps brachii in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-22
Question
I see that the anti-SKIL/Snon antibody A04131 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-12-15
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-12-15
Question
My question regarding product A04131, anti-SKIL/Snon antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
B. Lewis
Verified customer
Asked: 2017-04-25
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04131 anti-SKIL/Snon antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-04-25
Question
See attached the WB image, lot number and protocol we used for tendon of biceps brachii using anti-SKIL/Snon antibody A04131. Please let me know if you require anything else.
V. Parker
Verified customer
Asked: 2016-09-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-09-20
Question
Does anti-SKIL/Snon antibody A04131 work on monkey WB with tendon of biceps brachii?
E. Gonzalez
Verified customer
Asked: 2013-12-16
Answer
Our lab technicians have not tested anti-SKIL/Snon antibody A04131 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-SKIL/Snon antibody A04131 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey tendon of biceps brachii in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-12-16
Question
Does anti-SKIL/Snon antibody A04131 work for WB with tendon of biceps brachii?
S. Banerjee
Verified customer
Asked: 2013-06-28
Answer
According to the expression profile of tendon of biceps brachii, SKIL is highly expressed in tendon of biceps brachii. So, it is likely that anti-SKIL/Snon antibody A04131 will work for WB with tendon of biceps brachii.
Boster Scientific Support
Answered: 2013-06-28
