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Product Info Summary
| SKU: | M03630-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Mouse |
| Application: | ELISA, Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-B-cell linker protein BLNK Monoclonal Antibody
SKU/Catalog Number
M03630-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-B-cell linker protein BLNK Monoclonal Antibody catalog # M03630-1. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-B-cell linker protein BLNK Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03630-1)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
5G9
Isotype
IgG
Immunogen
Purified recombinant fragment of human BLNK expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M03630-1 is reactive to BLNK in Human, Mouse
Reconstitution
Calculated molecular weight
50466 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03630-1 is guaranteed for ELISA, Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:200-1:1000
IF 1:200-1:1000
FC 1:200-1:400
ELISA 1:10000
Validation Images & Assay Conditions
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Figure 5. ELISA validation of BLNK using Anti-B-cell linker protein BLNK Monoclonal Antibody (M03630-1).
ELISA analysis of BLNK antibody.
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Figure 4. Flow Cytometry validation of BLNK using Anti-B-cell linker protein BLNK Monoclonal Antibody (M03630-1).
Flow cytometric (FCM) analysis of NIH/3T3 cells using BLNK Monoclonal Antibody (green) and negative control (purple).
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Figure 3. Immunofluorescent staining data of BLNK using Anti-B-cell linker protein BLNK Monoclonal Antibody (M03630-1).
Immunofluorescence (IF) analysis of HepG2 cells using BLNK Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Figure 2. Immunohistochemistry validation of BLNK using Anti-B-cell linker protein BLNK Monoclonal Antibody (M03630-1).
Immunohistochemistry (IHC) analysis of paraffin-embedded human cervical cancer tissues with DAB staining using BLNK Monoclonal Antibody.For more protocol information of IHC
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Figure 1. Western blotting validation for Anti-B-cell linker protein BLNK Monoclonal Antibody M03630-1
Western Blot (WB) analysis using BLNK Monoclonal Antibody against NIH/3T3 (1) and BCBL-1 (2) cell lysate.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For BLNK (Source: Uniprot.org, NCBI)
Gene Name
BLNK
Full Name
B-cell linker protein
Weight
50466 MW
Alternative Names
B-cell linker protein;B-cell adapter containing a SH2 domain protein;B-cell adapter containing a Src homology 2 domain protein;Cytoplasmic adapter protein;Src homology 2 domain-containing leukocyte protein of 65 kDa;SLP-65;BLNK;BASH, SLP65; Blnk|BA, BASH, BC, BL, Bca, Ly-5, Ly-57, Ly5, Ly57, Lyw-57, SLP, SLP-65|B cell linker|B-cell linker protein|B-cell adapter containing a SH2 domain protein|B-cell adapter containing a Src homology 2 domain protein|cytoplasmic adapter protein|lymphocyte 57|src homology 2 domain-containing leukocyte protein of 65 kDa
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BLNK, check out the BLNK Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BLNK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-B-cell linker protein BLNK Monoclonal Antibody (M03630-1)
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