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Product Info Summary
| SKU: | M00661 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, IHC-F, Functional Assay |
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Product info
Product Name
Anti-Hu CD314 Purified Azide Free KLRK1 Monoclonal Antibody
View all NKG2D/CD314 Antibodies
SKU/Catalog Number
M00661
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD314 Purified Azide Free KLRK1 Monoclonal Antibody (Catalog# M00661). Tested in Flow Cytometry, IP, IHC-F, Functional Assay application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD314 Purified Azide Free KLRK1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00661)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, azide-free
Clonality
Monoclonal
Clone Number
1D11
Isotype
Mouse IgG1 kappa
Immunogen
NKL cell line. The mouse monoclonal antibody 1D11 recognizes an extracellular epitope of CD314 / NKG2D, a 42 kDa C-type lectin-like activating receptor expressed by NK cells, gamma/delta T cells, and CD8+ T cells.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00661 is reactive to KLRK1 in Human
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
25274 MW
Background of NKG2D/CD314
CD314, also known as NKG2D (natural killer receptor G2D) or KLRK1 (killer cell lectin-like receptor subfamily K, member 1), is a homodimeric C-type lectin-like activating receptor and costimulator with type II membrane orientation (C teminus extracellular). CD314 homodimers are associated with DAP10, a membrane adaptor protein that signals similar to CD28 by recruitment of phosphatidylinositol 3-kinase. Engagement of CD314 amplifies antigen-specific T cell responses in CD314-positive T cell populations. In NK cells, CD314 is a primary activating receptor. As CD314 ligands the MHC class-I chain-related proteins A and B (MICA, MICB) and UL16-binding proteins (ULBPs) have been identified.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00661 is guaranteed for Flow Cytometry, IP, IHC-F, Functional Assay Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Functional application: Blocking of ligand binding.
Immunohistochemistry: 5-10 μg/ml.
Flow cytometry: 1-4 µg/ml
Validation Images & Assay Conditions
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Figure 1. Flow Cytometry validation of KLRK1 using Anti-Hu CD314 Purified Azide Free KLRK1 Monoclonal Antibody (M00661).
Flow cytometry surface staining pattern of human peripheral blood cells stained using anti-human CD314 (1D11) purified antibody (azide free
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Figure 2. Flow Cytometry validation of KLRK1 using Anti-Hu CD314 Purified Azide Free KLRK1 Monoclonal Antibody (M00661).
Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD314 (1D11) purified antibody (azide free
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Figure 3. Flow Cytometry validation of KLRK1 using Anti-Hu CD314 Purified Azide Free KLRK1 Monoclonal Antibody (M00661).
Separation of human CD314 positive CD56 positive NK cells (red-filled) from CD314 negative CD56 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD314 (1D11) purified antibody (azide freeProtein Target Info & Infographic
Gene/Protein Information For KLRK1 (Source: Uniprot.org, NCBI)
Gene Name
KLRK1
Full Name
NKG2-D type II integral membrane protein
Weight
25274 MW
Alternative Names
NKG2D, KLRK1, KLR KLRK1 CD314, D12S2489E, KLR, NKG2-D, NKG2D killer cell lectin like receptor K1 NKG2-D type II integral membrane protein|NK cell receptor D|NKG2-D-activating NK receptor|killer cell lectin-like receptor subfamily K, member 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KLRK1, check out the KLRK1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KLRK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Hu CD314 Purified Azide Free KLRK1 Monoclonal Antibody (M00661)
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