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Product Info Summary
| SKU: | A00109 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-PD-L1/B7-H1/CD274 Antibody Picoband®
SKU/Catalog Number
A00109
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PD-L1/B7-H1/CD274 Antibody Picoband® catalog # A00109. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PD-L1/B7-H1/CD274 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00109)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human PD-L1/B7-H1, different from the related mouse sequence by ten amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00109 is reactive to CD274 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
62 kDa
Calculated molecular weight
33275 MW
Background of PD-L1
Programmed death-ligand 1 (PD-L1), also known as CD274 or B7-H1, is a protein that in humans is encoded by the CD274 gene. It is mapped to 9p24.1. PD-L1 is a 40kDa type 1 transmembrane protein that has been speculated to play a major role in suppressing the immune system during particular events such as pregnancy, tissue allografts, autoimmune disease and other disease states such as hepatitis. It has been concluded that upregulation of PD-L1 on tumor MDCs downregulates T-cell immunity and that PD-L1 blockade may represent an approach for cancer immunotherapy. Additionally, PD-L1 can provide positive costimulatory signals for innate and adaptive immunity and for protection against intracellular bacterial infection. What’s more, it has been found that PD1/PDL1 pathway may be a good target for restoring antitumor immunity in ovarian cancer.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00109 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: HELA whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of PD-L1/B7-H1 using anti-PD-L1/B7-H1 antibody (A00109).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: HELA whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PD-L1/B7-H1 antigen affinity purified polyclonal antibody (Catalog # A00109) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PD-L1/B7-H1 at approximately 62KD. The expected band size for PD-L1/B7-H1 is at 33KD.
Protein Target Info & Infographic
Gene/Protein Information For CD274 (Source: Uniprot.org, NCBI)
Gene Name
CD274
Full Name
Programmed cell death 1 ligand 1
Weight
33275 MW
Superfamily
immunoglobulin superfamily
Alternative Names
Programmed cell death 1 ligand 1;PD-L1;PDCD1 ligand 1;Programmed death ligand 1;B7 homolog 1;B7-H1;CD274;CD274;B7H1, PDCD1L1, PDCD1LG1, PDL1; CD274 B7-H, B7H1, PD-L1, PDCD1L1, PDCD1LG1, PDL1, hPD-L1 CD274 molecule programmed cell death 1 ligand 1|B7 homolog 1|CD274 |PDCD1 ligand 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD274, check out the CD274 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD274: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PD-L1/B7-H1/CD274 Antibody Picoband® (A00109)
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No publications found for A00109
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-PD-L1/B7-H1/CD274 Antibody Picoband®
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for gastric carcinoma using anti-PD-L1/B7-H1/CD274 antibody A00109. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-25
Question
I would like to test anti-PD-L1/B7-H1/CD274 antibody A00109 on human gastric carcinoma for research purposes, then I may be interested in using anti-PD-L1/B7-H1/CD274 antibody A00109 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-30
Answer
The products we sell, including anti-PD-L1/B7-H1/CD274 antibody A00109, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-30
Question
Will A00109 anti-PD-L1/B7-H1/CD274 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-27
Answer
You can see on the product datasheet, A00109 anti-PD-L1/B7-H1/CD274 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-27
Question
See below the WB image, lot number and protocol we used for gastric carcinoma using anti-PD-L1/B7-H1/CD274 antibody A00109. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-10
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-10
Question
We are currently using anti-PD-L1/B7-H1/CD274 antibody A00109 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-29
Answer
The anti-PD-L1/B7-H1/CD274 antibody (A00109) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-29
Question
Is a blocking peptide available for product anti-PD-L1/B7-H1/CD274 antibody (A00109)?
Verified Customer
Verified customer
Asked: 2019-10-30
Answer
We do provide the blocking peptide for product anti-PD-L1/B7-H1/CD274 antibody (A00109). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-10-30
Question
I see that the anti-PD-L1/B7-H1/CD274 antibody A00109 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-28
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-28
Question
We were happy with the WB result of your anti-PD-L1/B7-H1/CD274 antibody. However we have seen positive staining in chorionic villus cell membrane using this antibody. Is that expected? Could you tell me where is CD274 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-10-25
Answer
From what I have seen in literature, chorionic villus does express CD274. Generally CD274 expresses in cell membrane. Regarding which tissues have CD274 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218
Lung, Pubmed ID: 15489334
Placenta, Pubmed ID: 11015443, 14702039
Boster Scientific Support
Answered: 2019-10-25
Question
Do you have a BSA free version of anti-PD-L1/B7-H1/CD274 antibody A00109 available?
Verified Customer
Verified customer
Asked: 2019-09-06
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-PD-L1/B7-H1/CD274 antibody A00109 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-06
Question
We have been able to see staining in human liver. Any tips? Is anti-PD-L1/B7-H1/CD274 antibody supposed to stain liver positively?
Verified Customer
Verified customer
Asked: 2018-04-19
Answer
From what I have seen in literature liver does express CD274. From what I have seen in Uniprot.org, CD274 is expressed in chorionic villus, placenta, gastric carcinoma, lung, liver, among other tissues. Regarding which tissues have CD274 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218
Lung, Pubmed ID: 15489334
Placenta, Pubmed ID: 11015443, 14702039
Boster Scientific Support
Answered: 2018-04-19
Question
Does anti-PD-L1/B7-H1/CD274 antibody A00109 work for WB with gastric carcinoma?
Verified Customer
Verified customer
Asked: 2017-12-11
Answer
According to the expression profile of gastric carcinoma, CD274 is highly expressed in gastric carcinoma. So, it is likely that anti-PD-L1/B7-H1/CD274 antibody A00109 will work for WB with gastric carcinoma.
Boster Scientific Support
Answered: 2017-12-11
Question
I was wanting to use your anti-PD-L1/B7-H1/CD274 antibody for WB for human gastric carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human gastric carcinoma identification?
Verified Customer
Verified customer
Asked: 2017-10-02
Answer
It shows on the product datasheet, A00109 anti-PD-L1/B7-H1/CD274 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human gastric carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-10-02
Question
I am interested in using your anti-PD-L1/B7-H1/CD274 antibody for negative regulation of interleukin-10 production studies. Has this antibody been tested with western blotting on hela whole cell lysates? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2017-09-14
Answer
Thank you for your inquiry. This A00109 anti-PD-L1/B7-H1/CD274 antibody is validated on hela whole cell lysates. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-09-14
Question
Can you help my question with product A00109, anti-PD-L1/B7-H1/CD274 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Z. Miller
Verified customer
Asked: 2016-07-06
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00109 anti-PD-L1/B7-H1/CD274 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-07-06
Question
Is this A00109 anti-PD-L1/B7-H1/CD274 antibody reactive to the isotypes of CD274?
D. Johnson
Verified customer
Asked: 2015-03-30
Answer
The immunogen of A00109 anti-PD-L1/B7-H1/CD274 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human PD-L1/B7-H1 (41-69aa KFPVEKQLDLAALIVYWEMEDKNIIQFVH), different from the related mouse sequence by ten amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-03-30
