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Product Info Summary
| SKU: | M00109 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-PD-L1 (CD274) Rabbit Monoclonal Antibody
SKU/Catalog Number
M00109
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PD-L1 (CD274) Rabbit Monoclonal Antibody catalog # M00109. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PD-L1 (CD274) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00109)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
IDC-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PD-L1 (CD274)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00109 is reactive to CD274 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
40 kDa
Calculated molecular weight
33275 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00109 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:100-1:500
IHC 1:50-1:100
Positive Control
WB: human Ramos whole cell, human HepG2 whole cell, human THP-1 whole cell, human K562 whole cell, rat thymus tissue, mouse thymus tissue
IHC: human thymoma tissue
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human thymoma, using PD-L1 (CD274) Antibody.
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Figure 1. Western blot analysis of PD-L1 using anti-PD-L1 antibody (M00109).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Ramos whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human THP-1 whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: rat thymus tissue lysates,
Lane 6: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PD-L1 antigen affinity purified monoclonal antibody (Catalog # M00109) at 1:100 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PD-L1 at approximately 40 kDa. The expected band size for PD-L1 is at 33 kDa.
Protein Target Info & Infographic
Gene/Protein Information For CD274 (Source: Uniprot.org, NCBI)
Gene Name
CD274
Full Name
Programmed cell death 1 ligand 1
Weight
33275 MW
Superfamily
immunoglobulin superfamily
Alternative Names
Programmed cell death 1 ligand 1;PD-L1;PDCD1 ligand 1;Programmed death ligand 1;B7 homolog 1;B7-H1;CD274;CD274;B7H1, PDCD1L1, PDCD1LG1, PDL1; CD274 B7-H, B7H1, PD-L1, PDCD1L1, PDCD1LG1, PDL1, hPD-L1 CD274 molecule programmed cell death 1 ligand 1|B7 homolog 1|CD274 |PDCD1 ligand 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD274, check out the CD274 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD274: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PD-L1 (CD274) Rabbit Monoclonal Antibody (M00109)
Hello CJ!
M00109 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
He J,Zhang W,Di T,Meng J,Qi Y,Li G,Zhang Y,Su H,Yan W.Water extract of sporoderm-broken spores of Ganoderma lucidum enhanced pd-l1 antibody efficiency through downregulation and relieved complications of pd-l1 monoclonal antibody.Biomed Pharmacother.2020
Species: Human,Mouse
M00109 usage in article: APP:IHC, SAMPLE:SPLEEN,HEART,LUNG AND KIDNEY, DILUTION:NA
CD25 and TGF-%u03B2 blockade based on predictive integrated immune ratio inhibits tumor growth in pancreatic cancer
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Customer Reviews
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Customer Q&As
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17 Customer Q&As for Anti-PD-L1 (CD274) Rabbit Monoclonal Antibody
Question
I see that the anti-PD-L1 (CD274) Rabbit Monoclonal antibody M00109 works with IHC-P, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-09
Answer
You can find protocols for IHC-P on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-04-09
Question
Our lab used your anti-PD-L1 (CD274) Rabbit Monoclonal antibody for IHC-P on placenta in a previous project. I am using rat, and I plan to use the antibody for WB next. I would like examining placenta as well as gastric carcinoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2020-03-06
Answer
I have checked the website and datasheets of our anti-PD-L1 (CD274) Rabbit Monoclonal antibody and it appears that M00109 has been tested on rat in both IHC-P and WB. Thus M00109 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-03-06
Question
We are currently using anti-PD-L1 (CD274) Rabbit Monoclonal antibody M00109 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-27
Answer
The anti-PD-L1 (CD274) Rabbit Monoclonal antibody (M00109) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-27
Question
What dilution factor was used in the WB test image on the product page?
Verified Customer
Verified customer
Asked: 2019-12-05
Answer
The dilution factor used in the WB image is 1:500.
Boster Scientific Support
Answered: 2019-12-05
Question
I was wanting to use your anti-PD-L1 (CD274) Rabbit Monoclonal antibody for IHC-P for rat placenta on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat placenta identification?
Verified Customer
Verified customer
Asked: 2019-11-01
Answer
It shows on the product datasheet, M00109 anti-PD-L1 (CD274) Rabbit Monoclonal antibody has been tested for IHC-P, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat placenta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-01
Question
We have seen staining in rat lung. What should we do? Is anti-PD-L1 (CD274) Rabbit Monoclonal antibody supposed to stain lung positively?
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
From what I have seen in literature lung does express CD274. From what I have seen in Uniprot.org, CD274 is expressed in chorionic villus, placenta, gastric carcinoma, lung, liver, among other tissues. Regarding which tissues have CD274 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218
Lung, Pubmed ID: 15489334
Placenta, Pubmed ID: 11015443, 14702039
Boster Scientific Support
Answered: 2019-09-11
Question
I have attached the WB image, lot number and protocol we used for placenta using anti-PD-L1 (CD274) Rabbit Monoclonal antibody M00109. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-29
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-29
Question
Does M00109 anti-PD-L1 (CD274) Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-29
Answer
It shows on the product datasheet, M00109 anti-PD-L1 (CD274) Rabbit Monoclonal antibody as been validated on IHC-P. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-29
Question
Is there a BSA free version of anti-PD-L1 (CD274) Rabbit Monoclonal antibody M00109 available?
Verified Customer
Verified customer
Asked: 2019-07-10
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PD-L1 (CD274) Rabbit Monoclonal antibody M00109 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-10
Question
Does anti-PD-L1 (CD274) Rabbit Monoclonal antibody M00109 work for IHC-P with placenta?
Verified Customer
Verified customer
Asked: 2019-05-17
Answer
According to the expression profile of placenta, CD274 is highly expressed in placenta. So, it is likely that anti-PD-L1 (CD274) Rabbit Monoclonal antibody M00109 will work for IHC-P with placenta.
Boster Scientific Support
Answered: 2019-05-17
Question
My question regarding product M00109, anti-PD-L1 (CD274) Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
B. Anderson
Verified customer
Asked: 2018-11-07
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00109 anti-PD-L1 (CD274) Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-11-07
Question
We need to test anti-PD-L1 (CD274) Rabbit Monoclonal antibody M00109 on rat placenta for research purposes, then I may be interested in using anti-PD-L1 (CD274) Rabbit Monoclonal antibody M00109 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-07-09
Answer
The products we sell, including anti-PD-L1 (CD274) Rabbit Monoclonal antibody M00109, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-07-09
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-PD-L1 (CD274) Rabbit Monoclonal antibody M00109. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-02-26
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-02-26
Question
Is this M00109 anti-PD-L1 (CD274) Rabbit Monoclonal antibody reactive to the isotypes of CD274?
Verified Customer
Verified customer
Asked: 2017-12-26
Answer
The immunogen of M00109 anti-PD-L1 (CD274) Rabbit Monoclonal antibody is A synthesized peptide derived from human PD-L1 (CD274). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-12-26
Question
Has the antibody been tested in any knockout model system?
Verified Customer
Verified customer
Asked: 2017-10-20
Answer
This antibody hasn't been tested in any knockout model system.
Boster Scientific Support
Answered: 2017-10-20
Question
Is a blocking peptide available for product anti-PD-L1 (CD274) Rabbit Monoclonal antibody (M00109)?
A. Mangal
Verified customer
Asked: 2014-11-19
Answer
We do provide the blocking peptide for product anti-PD-L1 (CD274) Rabbit Monoclonal antibody (M00109). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-11-19
Question
My colleagues were happy with the WB result of your anti-PD-L1 (CD274) Rabbit Monoclonal antibody. However we have seen positive staining in lung cell membrane using this antibody. Is that expected? Could you tell me where is CD274 supposed to be expressed?
C. Moore
Verified customer
Asked: 2014-09-11
Answer
From literature, lung does express CD274. Generally CD274 expresses in cell membrane. Regarding which tissues have CD274 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218
Lung, Pubmed ID: 15489334
Placenta, Pubmed ID: 11015443, 14702039
Boster Scientific Support
Answered: 2014-09-11
