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Product Info Summary
| SKU: | PA1850 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CD1d Antibody Picoband®
SKU/Catalog Number
PA1850
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD1d Antibody catalog # PA1850. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD1d Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1850)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human CD1d.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1850 is reactive to CD1D in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
31 kDa
Calculated molecular weight
37717 MW
Background of CD1D
CD1D (CD1d molecule) also known as R3G1, Antigen-presenting glycoprotein CD1d, R3G1, CD1D, is the sole group-2 member of the CD1 family of major histocompatibility (MHC)-like glycoproteins. Expressed on the surface of various human antigen-presenting cells. They are related to the class I MHC molecules, and are involved in the presentation of lipid antigens to T cells. It is the only member of the group 2 CD1 molecules. CD1d presented lipid antigens activate a special class of T cells, known as Natural Killer T (NKT) cells. When activated, NKT cells rapidly produce Th1 and Th2 cytokines, typically represented by interferon-gamma and IL-4 production. Jayawardena-Wolf et al. described 2 different pathways of Cd1d trafficking to endosomal compartments in mouse cells. A tyrosine-based motif governs recycling between the plasma membrane and the endosome, while Cd1d associates, like major histocompatibility complex class II antigens, with the invariant chain, or II, in the endoplasmic reticulum. Both pathways enhance antigen presentation to Cd1d-restricted natural killer T cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1850 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: COLO320 Cell, HELA Cell, HT1080 Cell, JURKAT Cell
IHC: Human Mammary Cancer Tissue
Validation Images & Assay Conditions
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Anti-CD1d antibody, PA1850, Western blotting
Lane 1: COLO320 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: HT1080 Cell Lysate
Lane 4: JURKAT Cell Lysate
Click image to see more details
Anti-CD1d antibody, PA1850, IHC(P)
IHC(P): Human Mammary Cancer Tissue
Protein Target Info & Infographic
Gene/Protein Information For CD1D (Source: Uniprot.org, NCBI)
Gene Name
CD1D
Full Name
Antigen-presenting glycoprotein CD1d
Weight
37717 MW
Alternative Names
Antigen-presenting glycoprotein CD1d;R3G1;CD1d;CD1D; CD1D CD1A, R3, R3G1 CD1d molecule -presenting glycoprotein CD1d|CD1D , d polypeptide|HMC class I -like glycoprotein CD1D|T-cell surface glycoprotein CD1d|differentiation CD1-alpha-3|thymocyte CD1D
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD1D, check out the CD1D Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD1D: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD1d Antibody Picoband® (PA1850)
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No publications found for PA1850
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-CD1d Antibody Picoband®
Question
Would anti-CD1d antibody PA1850 work on feline IHC with pancreas?
Verified Customer
Verified customer
Asked: 2020-03-10
Answer
Our lab technicians have not tested anti-CD1d antibody PA1850 on feline. You can run a BLAST between feline and the immunogen sequence of anti-CD1d antibody PA1850 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline pancreas in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-10
Question
Is a blocking peptide available for product anti-CD1d antibody (PA1850)?
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
We do provide the blocking peptide for product anti-CD1d antibody (PA1850). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-02-04
Question
Does PA1850 anti-CD1d antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
E. Carter
Verified customer
Asked: 2019-09-10
Answer
As indicated on the product datasheet, PA1850 anti-CD1d antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-10
Question
I was wanting to use your anti-CD1d antibody for IHC for human leukemic t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human leukemic t-cell identification?
Verified Customer
Verified customer
Asked: 2019-04-19
Answer
You can see on the product datasheet, PA1850 anti-CD1d antibody has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-04-19
Question
We are currently using anti-CD1d antibody PA1850 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2017-06-28
Answer
The anti-CD1d antibody (PA1850) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-06-28
Question
Is this PA1850 anti-CD1d antibody reactive to the isotypes of CD1D?
O. Edwards
Verified customer
Asked: 2013-07-12
Answer
The immunogen of PA1850 anti-CD1d antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human CD1d(76-92aa FSDQQWETLQHIFRVYR). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-07-12
