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Product Info Summary
| SKU: | A09145-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-HOMER3 Antibody Picoband®
SKU/Catalog Number
A09145-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HOMER3 Antibody Picoband® catalog # A09145-1. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HOMER3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09145-1)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human HOMER3 recombinant protein (Position: R282-A360). Human HOMER3 shares 88.6% and 89.9% amino acid (aa) sequence identity with mouse and rat HOMER3, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A09145-1 is reactive to HOMER3 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
45 kDa
Calculated molecular weight
39836 MW
Background of HOMER3
Homer protein homolog 3 is a protein that in humans is encoded by the HOMER3 gene. This gene encodes a member of the HOMER family of postsynaptic density scaffolding proteins that share a similar domain structure consisting of an N-terminal Enabled/vasodilator-stimulated phosphoprotein homology 1 domain which mediates protein-protein interactions, and a carboxy-terminal coiled-coil domain and two leucine zipper motifs that are involved in self-oligomerization. The encoded protein binds numerous other proteins including group I metabotropic glutamate receptors, inositol 1,4,5-trisphosphate receptors and amyloid precursor proteins and has been implicated in diverse biological functions such as neuronal signaling, T-cell activation and trafficking of amyloid beta peptides.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09145-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Positive Control
WB: human PC-3 whole cell, human U251 whole cell, human A431 whole cell, rat brain tissue, rat lung tissuemouse brain tissuemouse lung tissue
IHC: human mammary cancer tissue, human intestinal cancer tissue, human lung cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of HOMER3 using anti-HOMER3 antibody (A09145-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates,
Lane 2: human U251 whole cell lysates,
Lane 3: human A431 whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: rat lung tissue lysates.
Lane 6: mouse brain tissue lysates.
Lane 7: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HOMER3 antigen affinity purified polyclonal antibody (Catalog # A09145-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HOMER3 at approximately 45 kDa. The expected band size for HOMER3 is at 40 kDa.
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Figure 4. IHC analysis of HOMER3 using anti-HOMER3 antibody (A09145-1).
HOMER3 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HOMER3 Antibody (A09145-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 2. IHC analysis of HOMER3 using anti-HOMER3 antibody (A09145-1).
HOMER3 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HOMER3 Antibody (A09145-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of HOMER3 using anti-HOMER3 antibody (A09145-1).
HOMER3 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HOMER3 Antibody (A09145-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For HOMER3 (Source: Uniprot.org, NCBI)
Gene Name
HOMER3
Full Name
Homer protein homolog 3
Weight
39836 MW
Superfamily
Homer family
Alternative Names
Homer protein homolog 3;Homer-3;HOMER3; HOMER3 HOMER-3, VESL3 homer scaffold protein 3 homer protein homolog 3|Homer, neuronal immediate early gene, 3|homer homolog 3|homer scaffolding protein 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HOMER3, check out the HOMER3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HOMER3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HOMER3 Antibody Picoband® (A09145-1)
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Customer Q&As
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5 Customer Q&As for Anti-HOMER3 Antibody Picoband®
Question
I was wanting to use to test anti-HOMER3 antibody A09145-1 on mouse brain for research purposes, then I may be interested in using anti-HOMER3 antibody A09145-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-17
Answer
The products we sell, including anti-HOMER3 antibody A09145-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-17
Question
Do you have a BSA free version of anti-HOMER3 antibody A09145-1 available?
Verified Customer
Verified customer
Asked: 2019-06-17
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-HOMER3 antibody A09145-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-17
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-HOMER3 antibody A09145-1. Let me know if you need anything else.
E. Lewis
Verified customer
Asked: 2018-06-15
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-06-15
Question
I have a question about product A09145-1, anti-HOMER3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
A. Yang
Verified customer
Asked: 2016-12-20
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A09145-1 anti-HOMER3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-12-20
Question
Does anti-HOMER3 antibody A09145-1 work for IHC with brain?
M. Banerjee
Verified customer
Asked: 2013-01-29
Answer
According to the expression profile of brain, HOMER3 is highly expressed in brain. So, it is likely that anti-HOMER3 antibody A09145-1 will work for IHC with brain.
Boster Scientific Support
Answered: 2013-01-29
