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Product Info Summary
| SKU: | PB9120 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Hamartin/TSC1 Antibody Picoband®
SKU/Catalog Number
PB9120
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Hamartin/TSC1 Antibody Picoband® catalog # PB9120. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Hamartin/TSC1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9120)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Hamartin recombinant protein (Position: D686-Y884). Human Hamartin shares 96% and 95% amino acid (aa) sequences identity with mouse and rat Hamartin, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9120 is reactive to TSC1 in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
130 kDa
Calculated molecular weight
129767 MW
Background of TSC1
Hamartin also known as tuberous sclerosis 1 is a protein that in humans is encoded by the TSC1 gene. It is mapped to 9q34.13. This peripheral membrane protein was implicated as a tumor suppressor. It forms a complex with TSC2 that regulates mTORC1 signaling and may be also involved in vesicular transport and docking. Hamartin and TSC2 have critical roles in neuronal polarity, and that a common pathway regulates polarization and growth in neurons and cell size in other tissues. Hamartin is a growth inhibitory protein whose biologic effect is probably dependent on its interaction with tuberin. It also can affect cell proliferation via deregulation of G1 phase. Loss or perturbation of Hamartin function leads to loss of adhesion to the cellular matrix and initiates the development of TSC hamartomas.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9120 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: rat brain tissue, human Hela whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Hamartin using anti-Hamartin antibody (PB9120).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant human Hamartin protein 0.5 ng
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hamartin antigen affinity purified polyclonal antibody (Catalog # PB9120) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hamartin at approximately 38 kDa. The expected band size for Hamartin is at 38 kDa.
Click image to see more details
Figure 2. Western blot analysis of Hamartin using anti-Hamartin antibody (PB9120).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hamartin antigen affinity purified polyclonal antibody (Catalog # PB9120) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hamartin at approximately 130 kDa. The expected band size for Hamartin is at 130 kDa.
Protein Target Info & Infographic
Gene/Protein Information For TSC1 (Source: Uniprot.org, NCBI)
Gene Name
TSC1
Full Name
Hamartin
Weight
129767 MW
Alternative Names
Hamartin;Tuberous sclerosis 1 protein;TSC1;KIAA0243, TSC; TSC1 LAM, TSC TSC complex subunit 1 hamartin|truncated hemartin|tuberous sclerosis 1 protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TSC1, check out the TSC1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TSC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Hamartin/TSC1 Antibody Picoband® (PB9120)
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No publications found for PB9120
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Customer Q&As
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16 Customer Q&As for Anti-Hamartin/TSC1 Antibody Picoband®
Question
I have a question about product PB9120, anti-Hamartin/TSC1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
R. Kulkarni
Verified customer
Asked: 2020-04-15
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9120 anti-Hamartin/TSC1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-15
Question
I am looking for using your anti-Hamartin/TSC1 antibody for negative regulation of neuron projection development studies. Has this antibody been tested with western blotting on hela whole cell lysate? We would like to see some validation images before ordering.
F. Li
Verified customer
Asked: 2020-02-14
Answer
We appreciate your inquiry. This PB9120 anti-Hamartin/TSC1 antibody is tested on rat brain tissue, tissue lysate, hela whole cell lysate. It is guaranteed to work for WB in human, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-02-14
Question
Is this PB9120 anti-Hamartin/TSC1 antibody reactive to the isotypes of TSC1?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
The immunogen of PB9120 anti-Hamartin/TSC1 antibody is E.coli-derived human Hamartin recombinant protein (Position: D686-Y884). Human Hamartin shares 96% and 95% amino acid (aa) sequences identity with mouse and rat Hamartin, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-13
Question
See attached the WB image, lot number and protocol we used for embryonic kidney using anti-Hamartin/TSC1 antibody PB9120. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-16
Question
I was wanting to use your anti-Hamartin/TSC1 antibody for WB for rat embryonic kidney on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat embryonic kidney identification?
Verified Customer
Verified customer
Asked: 2019-05-17
Answer
It shows on the product datasheet, PB9120 anti-Hamartin/TSC1 antibody has been tested for WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat embryonic kidney in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-17
Question
Is a blocking peptide available for product anti-Hamartin/TSC1 antibody (PB9120)?
N. Li
Verified customer
Asked: 2018-11-23
Answer
We do provide the blocking peptide for product anti-Hamartin/TSC1 antibody (PB9120). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-11-23
Question
We have observed staining in human blood. Do you have any suggestions? Is anti-Hamartin/TSC1 antibody supposed to stain blood positively?
Verified Customer
Verified customer
Asked: 2018-09-14
Answer
Based on literature blood does express TSC1. Based on Uniprot.org, TSC1 is expressed in cerebellum, testis, bone marrow, embryonic kidney, platelet, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, peripheral blood, blood, blood lymphoblast, peripheral blood leukocyte, among other tissues. Regarding which tissues have TSC1 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 9924605, 10570911
Blood, and Lymphoblast, Pubmed ID: 10533067
Bone marrow, Pubmed ID: 9039502
Cervix carcinoma, Pubmed ID: 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Peripheral blood, Pubmed ID: 9328481, 10227394
Peripheral blood leukocyte, Pubmed ID: 10607950, 10874311, 12112044
Platelet, Pubmed ID: 18088087
Testis, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2018-09-14
Question
Would PB9120 anti-Hamartin/TSC1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-03-20
Answer
You can see on the product datasheet, PB9120 anti-Hamartin/TSC1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-03-20
Question
Is there a BSA free version of anti-Hamartin/TSC1 antibody PB9120 available?
Verified Customer
Verified customer
Asked: 2017-07-26
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Hamartin/TSC1 antibody PB9120 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-07-26
Question
I see that the anti-Hamartin/TSC1 antibody PB9120 works with WB, what is the protocol used to produce the result images on the product page?
S. Banerjee
Verified customer
Asked: 2017-07-13
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-07-13
Question
We are currently using anti-Hamartin/TSC1 antibody PB9120 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, rat. Is it true that the antibody can work on bovine tissues as well?
B. Wu
Verified customer
Asked: 2017-07-11
Answer
The anti-Hamartin/TSC1 antibody (PB9120) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-07-11
Question
you antibody to test anti-Hamartin/TSC1 antibody PB9120 on rat embryonic kidney for research purposes, then I may be interested in using anti-Hamartin/TSC1 antibody PB9120 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
T. Zhao
Verified customer
Asked: 2016-03-23
Answer
The products we sell, including anti-Hamartin/TSC1 antibody PB9120, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-03-23
Question
Would anti-Hamartin/TSC1 antibody PB9120 work for WB with embryonic kidney?
V. Rodriguez
Verified customer
Asked: 2015-08-04
Answer
According to the expression profile of embryonic kidney, TSC1 is highly expressed in embryonic kidney. So, it is likely that anti-Hamartin/TSC1 antibody PB9120 will work for WB with embryonic kidney.
Boster Scientific Support
Answered: 2015-08-04
Question
Will anti-Hamartin/TSC1 antibody PB9120 work on monkey WB with cervix carcinoma erythroleukemia?
B. Anderson
Verified customer
Asked: 2014-06-16
Answer
Our lab technicians have not validated anti-Hamartin/TSC1 antibody PB9120 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-Hamartin/TSC1 antibody PB9120 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey cervix carcinoma erythroleukemia in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-06-16
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for embryonic kidney using anti-Hamartin/TSC1 antibody PB9120. Let me know if you need anything else.
B. Brown
Verified customer
Asked: 2013-10-28
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-10-28
Question
My colleagues were content with the WB result of your anti-Hamartin/TSC1 antibody. However we have been able to see positive staining in bone marrow cytoplasm. using this antibody. Is that expected? Could you tell me where is TSC1 supposed to be expressed?
L. Carter
Verified customer
Asked: 2013-01-10
Answer
Based on literature, bone marrow does express TSC1. Generally TSC1 expresses in cytoplasm. Regarding which tissues have TSC1 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 9924605, 10570911
Blood, and Lymphoblast, Pubmed ID: 10533067
Bone marrow, Pubmed ID: 9039502
Cervix carcinoma, Pubmed ID: 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Peripheral blood, Pubmed ID: 9328481, 10227394
Peripheral blood leukocyte, Pubmed ID: 10607950, 10874311, 12112044
Platelet, Pubmed ID: 18088087
Testis, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2013-01-10
