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Product Info Summary
| SKU: | A00365 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-Hamartin TSC1 Antibody
SKU/Catalog Number
A00365
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hamartin TSC1 Antibody (Catalog # A00365). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
TSC1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Hamartin TSC1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00365)
Host
Rabbit
Contents
TSC1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
TSC1 antibody was raised against a 15 amino acid synthetic peptide from the middle region of human TSC1. The immunogen is located within amino acids 220 - 270 of TSC1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least two isoforms of TSC1 are known to exist; this antibody will detect both isoforms. TSC1 antibody is predicted to not cross-react with TSC2.
Reactive Species
A00365 is reactive to TSC1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
129767 MW
Background of TSC1
Tuberous sclerosis complex (TSC) is an autosomal dominant tumor syndrome caused by mutations in either of the TSC1 or TSC2 tumor suppressor genes. The products of these genes form a protein complex that indirectly decreases the signaling of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. TOR activity is stimulated by Rheb, a member of the Ras superfamily of G-proteins, when the GTP/GDP ratio bound to Rheb is high. Immunoprecipitated TSC1/TSC2 has been shown to stimulate Rheb GTPase activity in vitro, suggesting that the TSC1/TSC2 decreases the ability of Rheb to stimulate TOR activity. This is supported by experiments showing overexpression of TSC1 and TSC2 results in a significant decrease in the GTP/GDP ratio bound to Rheb and the inhibition of cell growth. A shorter 40 kDa isoform of TSC1 has been shown to exist but its function is unknown.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00365 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
TSC1 antibody can be used for the detection of TSC1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 2 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of TSC1 in EL4 cell lysate with TSC1 antibody at 1 μg/mL in the (A) absence and (B) presence of blocking peptide.
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Immunocytochemistry of TSC1 in EL4 cells with TSC1 antibody at 2 μg/mL
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Immunofluorescence of TSC1 in L1210 cells with TSC1 antibody at 10 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For TSC1 (Source: Uniprot.org, NCBI)
Gene Name
TSC1
Full Name
Hamartin
Weight
129767 MW
Alternative Names
LAM, TSC, KIAA0243, Hamartin, Tuberous sclerosis 1 protein TSC1 LAM, TSC TSC complex subunit 1 hamartin|truncated hemartin|tuberous sclerosis 1 protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TSC1, check out the TSC1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TSC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Hamartin TSC1 Antibody (A00365)
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