Anti-Hamartin/TSC1 Antibody Picoband®

Product Name

Anti-Hamartin/TSC1 Antibody Picoband®

View all TSC1 Antibodies

SKU/Catalog Number

PA2012

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Hamartin/TSC1 Antibody catalog # PA2012. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Hamartin/TSC1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2012)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Hamartin, identical to the related rat sequence, and different from the related mouse sequence by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2012 is reactive to TSC1 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

160 kDa

Calculated molecular weight

129767 MW

Background of TSC1

TSC1 (Tuberous Sclerosis 1), also called HAMARTIN or TSC, is a human protein and gene. As part of a comprehensive strategy to identify the gene mutant in tuberous sclerosis-1, van Slegtenhorst et al. (1997) developed an overlapping contig of clones from the 1.4-Mb TSC1 region on chromosome 9. Benvenuto et al. (2000) showed that overexpression of the TSC1 gene in rat fibroblasts inhibits growth and causes changes in cell morphology. Van Slegtenhorst et al. (1998) showed that hamartin and tuberin associate physically in vivo, however, and that the interaction is mediated by predicted coiled-coil domains.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

PA2012 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat

Positive Control

WB: Rat Skeletal Muscle Tissue, Rat Heart Tissue, Rat Brain Tissue, Rat Lung Tissue, 293T Whole Cell, HELA Whole Cell, HT1080 Whole Cell, SMMC Whole Cell
IHC: Human Mammary Cancer Tissue, Rat Brain Tissue

Validation Images & Assay Conditions

Gene/Protein Information For TSC1 (Source: Uniprot.org, NCBI)

Alternative Names

Hamartin;Tuberous sclerosis 1 protein;TSC1;KIAA0243, TSC; TSC1 LAM, TSC TSC complex subunit 1 hamartin|truncated hemartin|tuberous sclerosis 1 protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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