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Product Info Summary
| SKU: | A04068 |
|---|---|
| Size: | 100 µg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-SAPK interacting protein 1/Mip1 MAPKAP1 Antibody
View all Sin1/MAPKAP1 Antibodies
SKU/Catalog Number
A04068
Size
100 µg
Form
Liquid
Description
Boster Bio Anti-SAPK interacting protein 1/Mip1 MAPKAP1 Antibody (Catalog# A04068). Tested in WB application(s). This antibody reacts with Human.
Storage & Handling
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Cite This Product
Anti-SAPK interacting protein 1/Mip1 MAPKAP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04068)
Host
Rabbit
Contents
100 μg in 200 μl PBS containing 0.05% BSA and 0.05% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A partial length recombinant Mip1 protein (amino acids 250-522)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A04068 is reactive to MAPKAP1 in Human
Reconstitution
Calculated molecular weight
59123 MW
Background of Sin1/MAPKAP1
SmarcAL1 (SWI/SNF-related µatrix-associated actin-dependent regulator of chromatin a-like 1) is also known as HARP, HepA Related Protein, HHARP, SMARCA like Protein 1 and SWI/SNF Related. SmarcAL1 is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein shows sequence similarity to the E. coli RNA polymerase-binding protein HepA. Mutations in this gene are a cause of Schimke immunoosseous dysplasia (SIOD), an autosomal recessive disorder with the diagnostic features of spondyloepiphyseal dysplasia, renal dysfunction, and T-cell immunodeficiency.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04068 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot: 1-3 µg/ml
Validation Images & Assay Conditions
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Western blot analysis of SAPK interacting protein 1/Mip1. Anti-SAPK interacting protein 1/Mip1 antibody was used at 1 &mu
Protein Target Info & Infographic
Gene/Protein Information For MAPKAP1 (Source: Uniprot.org, NCBI)
Gene Name
MAPKAP1
Full Name
Target of rapamycin complex 2 subunit MAPKAP1
Weight
59123 MW
Superfamily
SIN1 family
Alternative Names
Target of rapamycin complex 2 subunit MAPKAP1;TORC2 subunit MAPKAP1;Mitogen-activated protein kinase 2-associated protein 1;Stress-activated map kinase-interacting protein 1;SAPK-interacting protein 1;mSIN1;MAPKAP1;MIP1, SIN1; MAPKAP1 JC310, MIP1, SIN1, SIN1b, SIN1g MAPK associated protein 1 target of rapamycin complex 2 subunit MAPKAP1|MEKK2-interacting protein 1|SAPK-interacting protein 1|TORC2 subunit MAPKAP1|mSIN1|mitogen-activated protein kinase 2-associated protein 1|mitogen-activated protein kinase associated protein 1|ras inhibitor MGC2745|stress-activated map kinase interacting protein 1|stress-activated protein kinase-interacting 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAPKAP1, check out the MAPKAP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAPKAP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SAPK interacting protein 1/Mip1 MAPKAP1 Antibody (A04068)
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No publications found for A04068
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Customer Q&As
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13 Customer Q&As for Anti-SAPK interacting protein 1/Mip1 MAPKAP1 Antibody
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for urinary bladder using anti-SAPK interacting protein 1/Mip1 antibody A04068. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-24
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-24
Question
Does A04068 anti-SAPK interacting protein 1/Mip1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-16
Answer
As indicated on the product datasheet, A04068 anti-SAPK interacting protein 1/Mip1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-16
Question
I see that the anti-SAPK interacting protein 1/Mip1 antibody A04068 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-03-11
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-03-11
Question
Is this A04068 anti-SAPK interacting protein 1/Mip1 antibody reactive to the isotypes of MAPKAP1?
Verified Customer
Verified customer
Asked: 2020-02-27
Answer
The immunogen of A04068 anti-SAPK interacting protein 1/Mip1 antibody is A partial length recombinant Mip1 protein (amino acids 250-522). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-27
Question
I was wanting to use your anti-SAPK interacting protein 1/Mip1 antibody for WB for human urinary bladder on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human urinary bladder identification?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
As indicated on the product datasheet, A04068 anti-SAPK interacting protein 1/Mip1 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human urinary bladder in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-23
Question
Please see the WB image, lot number and protocol we used for urinary bladder using anti-SAPK interacting protein 1/Mip1 antibody A04068. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-16
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-16
Question
My question regards to test anti-SAPK interacting protein 1/Mip1 antibody A04068 on human urinary bladder for research purposes, then I may be interested in using anti-SAPK interacting protein 1/Mip1 antibody A04068 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-08-27
Answer
The products we sell, including anti-SAPK interacting protein 1/Mip1 antibody A04068, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-08-27
Question
Is a blocking peptide available for product anti-SAPK interacting protein 1/Mip1 antibody (A04068)?
Verified Customer
Verified customer
Asked: 2018-01-15
Answer
We do provide the blocking peptide for product anti-SAPK interacting protein 1/Mip1 antibody (A04068). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-01-15
Question
Would anti-SAPK interacting protein 1/Mip1 antibody A04068 work on canine WB with glial cell?
M. Jones
Verified customer
Asked: 2015-12-28
Answer
Our lab technicians have not validated anti-SAPK interacting protein 1/Mip1 antibody A04068 on canine. You can run a BLAST between canine and the immunogen sequence of anti-SAPK interacting protein 1/Mip1 antibody A04068 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine glial cell in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-12-28
Question
Is there a BSA free version of anti-SAPK interacting protein 1/Mip1 antibody A04068 available?
R. Williams
Verified customer
Asked: 2015-08-07
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-SAPK interacting protein 1/Mip1 antibody A04068 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-08-07
Question
We are currently using anti-SAPK interacting protein 1/Mip1 antibody A04068 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on feline tissues as well?
M. Miller
Verified customer
Asked: 2015-05-22
Answer
The anti-SAPK interacting protein 1/Mip1 antibody (A04068) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-05-22
Question
Does anti-SAPK interacting protein 1/Mip1 antibody A04068 work for WB with urinary bladder?
E. Krishna
Verified customer
Asked: 2013-09-11
Answer
According to the expression profile of urinary bladder, MAPKAP1 is highly expressed in urinary bladder. So, it is likely that anti-SAPK interacting protein 1/Mip1 antibody A04068 will work for WB with urinary bladder.
Boster Scientific Support
Answered: 2013-09-11
Question
I have a question about product A04068, anti-SAPK interacting protein 1/Mip1 antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
F. Mangal
Verified customer
Asked: 2013-04-22
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04068 anti-SAPK interacting protein 1/Mip1 antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-04-22
