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Product Info Summary
| SKU: | A11548 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-GRP1 CYTH3 Antibody
View all Cytohesin 3 Antibodies
SKU/Catalog Number
A11548
Size
100μl
Form
Liquid
Description
Boster Bio Anti-GRP1 CYTH3 Antibody catalog # A11548. Tested in WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GRP1 CYTH3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11548)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human GRP1. AA range:351-400
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A11548 is reactive to CYTH3 in Human, Monkey, Mouse, Rat
Reconstitution
Calculated molecular weight
46349 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11548 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of specific cells using GRP1 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For CYTH3 (Source: Uniprot.org, NCBI)
Gene Name
CYTH3
Full Name
Cytohesin-3
Weight
46349 MW
Alternative Names
Cytohesin-3;ARF nucleotide-binding site opener 3;Protein ARNO3;General receptor of phosphoinositides 1;Grp1;PH, SEC7 and coiled-coil domain-containing protein 3;CYTH3;ARNO3, GRP1, PSCD3; CYTH3 ARNO3, GRP1, PSCD3, cytohesin-3 cytohesin 3 cytohesin-3|ARF nucleotide-binding site opener 3|PH, SEC7 and coiled-coil domain-containing protein 3|general receptor of phosphoinositides 1|pleckstrin homology, Sec7 and coiled-coil domains 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CYTH3, check out the CYTH3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CYTH3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GRP1 CYTH3 Antibody (A11548)
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No publications found for A11548
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Customer Q&As
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6 Customer Q&As for Anti-GRP1 CYTH3 Antibody
Question
Would A11548 anti-GRP1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
S. Kulkarni
Verified customer
Asked: 2020-01-14
Answer
It shows on the product datasheet, A11548 anti-GRP1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-14
Question
See below the WB image, lot number and protocol we used for metanephros using anti-GRP1 antibody A11548. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-16
Question
My question regarding product A11548, anti-GRP1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-11-16
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A11548 anti-GRP1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-11-16
Question
We are currently using anti-GRP1 antibody A11548 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, monkey, mouse, rat. Is it likely that the antibody can work on horse tissues as well?
A. Parker
Verified customer
Asked: 2017-09-28
Answer
The anti-GRP1 antibody (A11548) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-09-28
Question
Is there a BSA free version of anti-GRP1 antibody A11548 available?
A. Wu
Verified customer
Asked: 2015-02-20
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GRP1 antibody A11548 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-02-20
Question
Is this A11548 anti-GRP1 antibody reactive to the isotypes of CYTH3?
G. Miller
Verified customer
Asked: 2014-02-24
Answer
The immunogen of A11548 anti-GRP1 antibody is Synthesized peptide derived from the C-terminal region of human GRP1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-02-24
