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Product Info Summary
| SKU: | A04518 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-PIBF1 Antibody
SKU/Catalog Number
A04518
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-PIBF1 Antibody (Catalog # A04518). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
PIBF1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-PIBF1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04518)
Host
Rabbit
Contents
PIBF1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Rabbit polyclonal PIBF1 antibody was raised against a 15 amino acid peptide near the carboxy terminus of human PIBF1. The immunogen is located within amino acids 610 - 660 of PIBF1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Multiple isoforms of PIBF1 exists as a result of alternative splicing event.
Reactive Species
A04518 is reactive to PIBF1 in Human, Mouse, Rat
Applications
A04518 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
89805 MW
Background of PIBF1
PIBF1 is synthesized during pregnancy in response to progesterone by T lymphocytes. PIBF1 inhibits arachidonic acid release, controls NK activity, and modifies the cytokine balance exerting an anti-abortive effect. It contains a leucine zipper motif, a basic zipper sequence, a PEST sequence, a nuclear localization signal, an ER membrane retention signal and N-glycosylation and phosphorylation sites. PIBF1 is significantly higher in healthy pregnant women than in women at risk for premature pregnancy termination. Full-length PIBF1 is associated with the nucleus and functions as a transcription factor, whereas secretion of shorter forms which may act as cytokines is induced by activation of the cell.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
PIBF1 antibody can be used for detection of PIBF1 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of PIBF1 in human placenta tissue lysate with PIBF1 antibody at (A) 1 and (B) 2 μg/mL
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Immunohistochemistry of PBIF in human spleen tissue with PBIF antibody at 5 μg/ml.
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Immunofluorescence of PBIF in human spleen tissue with PBIF antibody at 20 μg/ml.
Protein Target Info & Infographic
Gene/Protein Information For PIBF1 (Source: Uniprot.org, NCBI)
Gene Name
PIBF1
Full Name
Progesterone-induced-blocking factor 1
Weight
89805 MW
Alternative Names
C13orf24; chromosome 13 open reading frame 24; KIAA1008; PIBF; PIBF1; progesterone immunomodulatory binding factor 1; progesterone-induced blocking factor 1; progesterone-induced-blocking factor 1; RP11-505F3.1 PIBF1 C13orf24, CEP90, JBTS33, PIBF progesterone immunomodulatory binding factor 1 progesterone-induced-blocking factor 1|centrosomal protein of 90 kDa
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PIBF1, check out the PIBF1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PIBF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PIBF1 Antibody (A04518)
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