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Product Info Summary
| SKU: | PA2230 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, ICC, WB |
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Product info
Product Name
Anti-Cortisol Binding Globulin/SERPINA6 Antibody Picoband®
View all Serpin A6/Cortisol Binding Globulin Antibodies
SKU/Catalog Number
PA2230
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Cortisol Binding Globulin/SERPINA6 Antibody catalog # PA2230. Tested in IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cortisol Binding Globulin/SERPINA6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2230)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Cortisol Binding Globulin.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2230 is reactive to SERPINA6 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
60 kDa
Calculated molecular weight
45141 MW
Background of Serpin A6/Cortisol Binding Globulin
Corticosteroid-binding globulin (CBG), also known as Serpin A6 is a protein that in humans is encoded by the SERPINA6 gene. It is mapped 14q32.13. This gene encodes an alpha-globulin protein with corticosteroid-binding properties. This is the major transport protein for glucorticoids and progestins in the blood of most vertebrates. The gene localizes to a chromosomal region containing several closely related serine protease inhibitors which may have evolved by duplication events.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2230 is guaranteed for IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunocytochemistry , 0.5-1μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human HL-60 whole cell, human A375 whole cell, human 293T whole cell
IHC: human liver cancer tissue
ICC: HepG2 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Cortisol Binding Globulin using anti-Cortisol Binding Globulin antibody (PA2230).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HL-60 whole cell lysates,
Lane 2: human A375 whole cell lysates,
Lane 3: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cortisol Binding Globulin antigen affinity purified polyclonal antibody (Catalog # PA2230) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cortisol Binding Globulin at approximately 60 kDa. The expected band size for Cortisol Binding Globulin is at 53 kDa.
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Figure 2. ICC analysis of Cortisol Binding Globulin using anti-Cortisol Binding Globulin antibody (PA2230).
Cortisol Binding Globulin was detected in an immunocytochemical section of HepG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1 μg/ml rabbit anti-Cortisol Binding Globulin Antibody (PA2230) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis of Cortisol Binding Globulin using anti-Cortisol Binding Globulin antibody (PA2230).
Cortisol Binding Globulin was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Cortisol Binding Globulin Antibody (PA2230) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For SERPINA6 (Source: Uniprot.org, NCBI)
Gene Name
SERPINA6
Full Name
Corticosteroid-binding globulin
Weight
45141 MW
Superfamily
serpin family
Alternative Names
Corticosteroid-binding globulin;CBG;Serpin A6;Transcortin;SERPINA6;CBG; SERPINA6 CBG serpin family A member 6 corticosteroid-binding globulin|serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 6|serpin A6|serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 6|transcortin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SERPINA6, check out the SERPINA6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SERPINA6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cortisol Binding Globulin/SERPINA6 Antibody Picoband® (PA2230)
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Customer Q&As
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1 Customer Q&As for Anti-Cortisol Binding Globulin/SERPINA6 Antibody Picoband®
Question
We are currently using anti-Cortisol Binding Globulin/SERPINA6 antibody PA2230 for human tissue, and we are happy with the IHC-P results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?
K. Krishna
Verified customer
Asked: 2018-08-21
Answer
The anti-Cortisol Binding Globulin/SERPINA6 antibody (PA2230) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-08-21
