Product Info Summary
SKU: | EK1963 |
---|---|
Size: | 96 wells/kit, with removable strips. |
Reactive Species: | Human |
Application: | ELISA |
Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) and urine. |
Product info
Product Name
Human Serpin A6 ELISA Kit PicoKine®
View all Serpin A6/Cortisol Binding Globulin ELISA kits
SKU/Catalog Number
EK1963
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Serpin A6 ELISA Kit PicoKine® (96 Tests). Quantitate Human SERPINA6 in cell culture supernatants, serum and plasma (heparin, EDTA) and urine. Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Serpin A6 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1963)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: M23-V405
Sensitivity
<50 pg/ml
Assay Range
1.56 ng/ml - 100 ng/ml
Standard Dilution Instructions
See datasheet of EK1963 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1963 is reactive to SERPINA6 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA) and urine.
Application Guarantee
EK1963 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Serpin A6/Cortisol Binding Globulin
Corticosteroid-binding globulin (CBG), also known as Serpin A6 is a protein that in humans is encoded by the SERPINA6 gene. It is mapped to 14q32.13. This gene encodes an alpha-globulin protein with corticosteroid-binding properties. This is the major transport protein for glucorticoids and progestins in the blood of most vertebrates. The gene localizes to a chromosomal region containing several closely related serine protease inhibitors which may have evolved by duplication events.
Kit Components
Catalog Number | Description | Quantity |
---|---|---|
EK1963-CAP | Anti-Human SERPINA6 Pre-coated 96-well strip microplate | 1 |
EK1963-ST | Human SERPINA6 Standard | 2 vials, 100 ng/tube |
EK1963-DA | Human SERPINA6 Biotinylated antibody (100x) | 100ul |
AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
AR1106-1 | Sample Diluent | 30ml |
AR1106-2 | Antibody Diluent | 12ml |
AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop Solution | 10ml |
AR1106-5 | Wash Buffer (25x) | 20ml |
PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
Concentration (ng/ml) | 0 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 | 100 |
O.D. | 0.033 | 0.107 | 0.176 | 0.228 | 0.356 | 0.620 | 1.091 | 1.987 |
Data Example Images
Click image to see more details
Human Serpin A6 PicoKine ELISA Kitstandard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Serpin A6/Cortisol Binding Globulin, Dilution ratio of 1:100, concentration in serum and plasma is 0.5-1 ug/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Serpin A6 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 2728 | 12106 | 43028 | 2986 | 12333 | 47148 |
Standard deviation | 141.86 | 544.77 | 2452.60 | 206.03 | 715.31 | 2970.32 |
CV (%) | 5.2% | 4.5% | 5.7% | 6.9% | 5.8% | 6.3% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Serpin A6/Cortisol Binding Globulin in ELISA kits from four different production batches/lots.
Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
---|---|---|---|---|---|---|---|
Sample 1 | 2728 | 3062 | 2675 | 2954 | 2854 | 159.04 | 5.5% |
Sample 2 | 12106 | 13177 | 12457 | 12922 | 12665 | 413.50 | 3.2% |
Sample 3 | 43028 | 43427 | 45382 | 41055 | 43223 | 1536.32 | 3.5% |
Protein Target Info & Infographic
Gene/Protein Information For SERPINA6 (Source: Uniprot.Org, NCBI)
Gene Name
SERPINA6
Full Name
Corticosteroid-binding globulin
Weight
45.141kDa
Superfamily
serpin family
Alternative Names
Corticosteroid-binding globulin; CBG; Serpin A6; Transcortin; SERPINA6; CBG SERPINA6 CBG serpin family A member 6 corticosteroid-binding globulin|serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 6|serpin A6|serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 6|transcortin
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on SERPINA6, check out the SERPINA6 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for SERPINA6: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Serpin A6 ELISA Kit PicoKine® (EK1963)
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Customer Reviews
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Customer Q&As
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17 Customer Q&As for Human Serpin A6 ELISA Kit PicoKine®
Question
What range of values with units are experimentally detectable in human plasma using EK1963?
Verified customer
Asked: 2021-01-13
Answer
For the Human Serpin A6 ELISA Kit PicoKine™ (EK1963), range value in human plasma is 0.1-0.5ug/ml.
Boster Scientific Support
Answered: 2021-01-14
Question
Is EK1963 quantitative?
Verified customer
Asked: 2021-01-13
Answer
The Human Serpin A6 ELISA Kit PicoKine™ (EK1963) is quantitative.
Boster Scientific Support
Answered: 2021-01-13
Question
Is EK1963 pre-coated?
Verified customer
Asked: 2021-01-13
Answer
The Human Serpin A6 ELISA Kit PicoKine™ (EK1963) is pre-coated.
Boster Scientific Support
Answered: 2021-01-13
Question
Has EK1963 been tested/validated on human plasma?
Verified customer
Asked: 2021-01-13
Answer
The Human Serpin A6 ELISA Kit PicoKine™ (EK1963) has been tested on human plasma.
Boster Scientific Support
Answered: 2021-01-13
Question
Has EK1963 been tested/validated on human plasma EDTA?
Verified customer
Asked: 2021-01-13
Answer
The Human Serpin A6 ELISA Kit PicoKine™ (EK1963) has been tested on human plasma EDTA.
Boster Scientific Support
Answered: 2021-01-13
Question
What is the range of values (with units) measured in human plasma specifically (not serum) for EK1963?
Verified customer
Asked: 2021-01-13
Answer
For the Human Serpin A6 ELISA Kit PicoKine™ (EK1963), the range of value is 0.1-1.5ug/ml.
Boster Scientific Support
Answered: 2021-01-13
Question
Do you make EK1963 yourself?
Verified customer
Asked: 2021-01-13
Answer
Yes, we make the Human Serpin A6 ELISA Kit PicoKine™ (EK1963) ourselves.
Boster Scientific Support
Answered: 2021-01-13
Question
Does EK1963 measure total concentration?
Verified customer
Asked: 2021-01-13
Answer
The Human Serpin A6 ELISA Kit PicoKine™ (EK1963) measures total concentration.
Boster Scientific Support
Answered: 2021-01-13
Question
Q: is it okay to use citrate plasma as samples in Human Serpin A6 Picokine® ELISA Kit (Catalog # EK1963)?
Verified Customer
Verified customer
Asked: 2020-09-23
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of Serpin A6 causing disruption of its protein structure. Serpin A6 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the Serpin A6 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-09-23
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
W. Evans
Verified customer
Asked: 2020-08-06
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-08-06
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement Serpin A6 using the EK1963 Human Serpin A6 Picokine® ELISA Kit?
P. Walker
Verified customer
Asked: 2020-04-29
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2020-04-29
Question
Q: What is the optimal O.D. value for Serpin A6 ELISA kit? I used your Serpin A6 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain Serpin A6 even though the O.D. values are not very high?
C. Anderson
Verified customer
Asked: 2020-02-25
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Serpin A6 ELISA kit to have sensitivity of 50pg/ml, that means the minimum amount of Serpin A6 that can be declared/interpreted as positive by the above standard is 50pg/ml.
Boster Scientific Support
Answered: 2020-02-25
Question
Q: how do I analyze ELISA data? I measured Serpin A6 level in serum.
Verified Customer
Verified customer
Asked: 2019-08-13
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-08-13
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
B. Miller
Verified customer
Asked: 2019-06-05
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-06-05
Question
Q: if the enzyme conjugated Serpin A6 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Serpin A6 antigen?
C. Nelson
Verified customer
Asked: 2017-12-30
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2017-12-30
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
G. Collins
Verified customer
Asked: 2017-03-14
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2017-03-14
Question
Q: which procedure should I follow in order to thaw whole blood sample for Serpin A6 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2016-11-27
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Serpin A6 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2016-11-27