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Key=sku, value=A00227-HRP
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Key=created_at, value=2020-03-11 06:18:51
Key=updated_at, value=2024-10-19 05:05:52
Key=mst_search_weight, value=0
Key=status, value=Enabled
Key=name, value=Anti-GAPDH Antibody Picoband® (HRP)
Key=cj_search_index
Key=publication_count, value=38
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Key=is_recurring, value=No
Key=visibility, value=Catalog, Search
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Key=conjugate, value=HRP
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Key=price, value=570.0000
Key=weight, value=1.0000
Key=meta_title, value=Anti-GAPDH Antibody (HRP)
Key=meta_description, value=Boster Bio Anti-GAPDH Antibody (HRP) catalog # A00227-HRP. Tested in ELISA, WB applications. This antibody reacts with Chicken, Human, Mouse, Rabbit, Rat, Zebrafish. Supplied as 0.1 mg in Liquid form antibody.
Key=image, value=/a/0/a00227-hrp-gapdh-primary-antibodies-wb-testing-1.jpg
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Key=url_key, value=anti-gapdh-antibody-hrp-a00227-hrp-boster
Key=url_path, value=anti-shank3-antibody-rp1109-boster
Key=options_container, value=Block after Info Column
Key=image_label, value=Figure 1. Western blot analysis of GAPDH using anti-GAPDH antibody (A00227-HRP).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of s
Key=small_image_label, value=Figure 1. Western blot analysis of GAPDH using anti-GAPDH antibody (A00227-HRP).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of s
Key=thumbnail_label, value=Figure 1. Western blot analysis of GAPDH using anti-GAPDH antibody (A00227-HRP).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of s
Key=msrp_display_actual_price_type, value=Use config
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Key=size, value=100μg/vial
Key=protein_name, value=Glyceraldehyde-3-phosphate dehydrogenase
Key=gene_full_name, value=Glyceraldehyde-3-phosphate dehydrogenase
Key=uniprot_id, value=P04406
Key=reconstitution, value=Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Key=sensitivity
Key=applications, value=WB
Key=reactivity, value=Chicken, Human, Monkey, Mouse, Rat, Zebrafish
Key=form, value=Liquid
Key=concentration, value=0.5 mg/mL
Key=cross_reactivity, value=HRP-GAPDH antibody is human, mouse, rat, rabbit, and chicken reactive.
Key=recommended_detection_systems, value=Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
Key=clone_number
Key=predicted_reactivity
Key=source_company, value=Boster A00227-HRP
Key=isotype, value=IgG
Key=molecular_weight, value=36053 MW
Key=sample_size_available, value=30ug for $99, contact us for details
Key=related_product_skus, value=B0001-5,AR1156,AR0146
Key=product_category, value=Primary Antibodies
Key=research_category, value=Neuroscience, Signal Transduction
Key=clonality, value=Polyclonal
Key=swatch_image, value=/a/0/a00227-hrp-gapdh-primary-antibodies-wb-testing-1.jpg
Key=rating_value, value=100
Key=review_count, value=5
Key=phospho_site
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Key=cj_related_products, value=Nuclear Receptors, Coactivators and Corepressors:A00227|A00227-1|M00227-4|M00227-6|M00227-Biotin|A00227-2
Key=out_of_stock_until_date, value=Nov 16, 2019
Key=description, value=Boster Bio Anti-GAPDH Antibody (HRP) (Catalog # A00227-HRP). Tested in WB applications. This antibody reacts with Human, Mouse, Rat, Monkey, Chicken, Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key=short_description, value=Boster Bio Anti-GAPDH Antibody (HRP) (Catalog # A00227-HRP). Tested in WB applications. This antibody reacts with Human, Mouse, Rat, Monkey, Chicken, Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key=meta_keyword, value=Anti-GAPDH Antibody (HRP)
Key=contents, value=Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4.
Key=background, value=Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. It also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export (1). GAPDH also plays a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer's and Huntington's disease (2). GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
Key=gene_name, value=GAPDH
Key=synonyms, value=HRP-GAPDH, Glyceraldehyde-3-phosphate dehydrogenase, G3PDH, GAPD
Key=kit_components, value=-
Key=application_details, value=Western blot, 0.02-0.1 μg/ml
Key=subcellular_localization, value=Cytoplasm, cytosol
Key=sequence_similarities, value=Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A4 subfamily.
Key=tissue_specificity, value=Widely expressed, with the highest expression in lung, spleen and thymus.
Key=application_notes, value=Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Key=description_after_attributes, value=

Cross-reacitivity

(ELISA)Recombinant bovine IL-4  (-)
(ELISA)Recombinant chicken IL-4  (-)
(ELISA)Recombinant feline IL-4  (-)
(ELISA)Recombinant human IL-4  (-)
(ELISA)Recombinant rabbit IL-4  (-)
(ELISA)Recombinant swine IL-4  (-)

Key=competitor_equivalent_skus, value=sc 23543|sc 23547|sc 30193|sc 377088|sc 377470|sc 393963
Key=immunogen, value=HRP-GAPDH antibody was raised against a synthetic peptide containing 16 amino acids near the carboxy terminus of GAPDH.
Key=purification, value=HRP-GAPDH antibody is affinity chromatography purified via peptide column.
Key=storage, value=HRP-GAPDH antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Key=reproducibility, value=-
Key=custom_attribute_1, value=36 kDa
Key=custom_attribute_2, value=WB: human Hela whole cell, human A549 whole cell, human A562 whole cell, human MCF-7 whole cell, rat brain tissue, rat liver tissue, mouse brain tissue, mouse liver tissue, chicken heart tissue, chicken liver tissue, chicken brain tissue, monkey heart tissue, monkey lung tissue, monkey kidney tissue, monkey liver tissue
Key=protein_function, value=Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D- glyceroyl phosphate. Component of the GAIT (gamma interferon- activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.
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