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Product Info Summary
| SKU: | M00227-Biotin |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rabbit, Rat |
| Host: | Mouse |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-GAPDH Monoclonal Antibody [12D3D5] (biotin)
SKU/Catalog Number
M00227-Biotin
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-GAPDH Monoclonal Antibody [12D3D5] (biotin) (Catalog # M00227-Biotin). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat, Rabbit.
Storage & Handling
Biotin-GAPDH antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-GAPDH Monoclonal Antibody [12D3D5] (biotin) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00227-Biotin)
Host
Mouse
Contents
Biotin-GAPDH antibody is supplied in PBS containing 1% BSA and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
Clone: 12D3D5
Isotype
IgG
Immunogen
Biotin-GAPDH antibody was raised against a 16 amino acid peptide near the carboxy terminus of human GAPDH.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Biotin-GAPDH antibody is human, mouse, rat, and rabbit reactive.
Reactive Species
M00227-Biotin is reactive to GAPDH in Human, Mouse, Rabbit, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
36053 MW
Background of GAPDH
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. It also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export (1). GAPDH also plays a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer's and Huntington's disease (2). GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00227-Biotin is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Biotin-GAPDH antibody can be used for detection of GAPDH by Western blot at 1 - 2 μg/ml.
Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of GAPDH in multiple cell and tissue lysates with Biotin-GAPDH antibody at 1 μg/ml. Lanes 1-20: 293
Protein Target Info & Infographic
Gene/Protein Information For GAPDH (Source: Uniprot.org, NCBI)
Gene Name
GAPDH
Full Name
Glyceraldehyde-3-phosphate dehydrogenase
Weight
36053 MW
Superfamily
glyceraldehyde-3-phosphate dehydrogenase family
Alternative Names
Glyceraldehyde-3-phosphate dehydrogenase, G3PDH, GAPD GAPDH G3PD, GAPD, HEL-S-162eP glyceraldehyde-3-phosphate dehydrogenase glyceraldehyde-3-phosphate dehydrogenase|OCAS, p38 component|Oct1 coactivator in S phase, 38 Kd component|aging-associated gene 9 protein|epididymis secretory sperm binding protein Li 162eP|peptidyl-cysteine S-nitrosylase GAPDH
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GAPDH, check out the GAPDH Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GAPDH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GAPDH Monoclonal Antibody [12D3D5] (biotin) (M00227-Biotin)
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