Product Info Summary
SKU: | M00227-4 |
---|---|
Size: | 100ul |
Reactive Species: | Bovine, Chicken, Equine, Human, Mouse, Pig, Rat |
Host: | Rabbit |
Application: | IF, IHC, WB |
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Product info
Product Name
Anti-GAPDH Antibody
SKU/Catalog Number
M00227-4
Size
100ul
Description
Boster Bio Anti-GAPDH Antibody catalog # M00227-4. Tested in IF, IHC, WB applications. This antibody reacts with Bovine, Chicken, Equine, Human, Mouse, Pig, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GAPDH Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00227-4)
Host
Rabbit
Contents
Antibody is supplied as an aliquot of serum
Clonality
Polyclonal
Immunogen
Native porcine purified protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00227-4 is reactive to Gapdh in Bovine, Chicken, Equine, Human, Mouse, Pig, Rat
Reconstitution
Calculated molecular weight
35828 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00227-4 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:30,000 IF/ICC and IHC: 1:10,000
Validation Images & Assay Conditions
Click image to see more details
Click image to see more details
Protein Target Info & Infographic
Gene/Protein Information For Gapdh (Source: Uniprot.org, NCBI)
Gene Name
Gapdh
Full Name
Glyceraldehyde-3-phosphate dehydrogenase
Weight
35828 MW
Superfamily
glyceraldehyde-3-phosphate dehydrogenase family
Alternative Names
Glyceraldehyde-3-phosphate dehydrogenase;GAPDH;1.2.1.12;38 kDa BFA-dependent ADP-ribosylation substrate;BARS-38;Peptidyl-cysteine S-nitrosylase GAPDH;2.6.99.-;Gapdh;Gapd; GAPDH G3PD, GAPD, HEL-S-162eP glyceraldehyde-3-phosphate dehydrogenase glyceraldehyde-3-phosphate dehydrogenase|OCAS, p38 component|Oct1 coactivator in S phase, 38 Kd component|aging-associated gene 9 protein|epididymis secretory sperm binding protein Li 162eP|peptidyl-cysteine S-nitrosylase GAPDH
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Gapdh, check out the Gapdh Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Gapdh: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GAPDH Antibody (M00227-4)
Hello CJ!
M00227-4 has been cited in 132 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Ma Y,Chai H,Ding Q,Qian Q,Yan Z,Ding B,Dou X,Li S.Hepatic SIRT3 Upregulation in Response to Chronic Alcohol Consumption Contributes to Alcoholic Liver Disease in Mice.Front Physiol.2019 Aug 13;10:1042.doi:10.3389/fphys.2019.01042.PMID:31474877;PMCID:PMC6707764.
Species: Human,Mouse
M00227-4 usage in article: APP:WB, SAMPLE:LIVER TISSUE, DILUTION:NA
Lu X,Yang B,Yu H,Hu X,Nie J,Wan B,Zhang M,Lü C.Epigenetic mechanisms underlying the effects of triptolide and tripchlorolide on the expression of neuroligin-1 in the hippocampus of APP/PS1 transgenic mice.Pharm Biol.2019 Dec;57(1):453-459.doi:10. 1080/13880209.2019.1629463.PMID:31311385;PMCID:PMC6691810.
Species: Mouse
M00227-4 usage in article: APP:WB, SAMPLE:HIPPOCAMPAL TISSUE, DILUTION:1:1000
Wei X,Bao Y,Zhan X,Zhang L,Hao G,Zhou J,Chen Q.MiR-200a ameliorates peritoneal fibrosis and functional deterioration in a rat model of peritoneal dialysis.Int Urol Nephrol.2019 May;51(5):889-896.doi:10.1007/s11255-019-02122-4.Epub 2019 Mar 19.PMID:30888602;PMCID:PMC6499761.
Species: Rat
M00227-4 usage in article: APP:WB, SAMPLE:PERITONEAL TISSUE, DILUTION:NA
Li,Z.,Wu,Z.,Yan,J.et al.Gut microbe-derived metabolite trimethylamine N-oxide induces cardiac hypertrophy and fibrosis.Lab Invest 99,346–357(2019).https://doi.org/10.1038/s41374-018-0091-y
Species: Rat
M00227-4 usage in article: APP:WB, SAMPLE:CARDIOMYOCYTES, DILUTION:1:10000
Zhao,Y.,Jia,W.,Ren,S.,Xiao,W.,Li,G.,Jin,L.,& Lin,Y.(2021). Difluoromethylornithine attenuates isoproterenol‑induced cardiac hypertrophy by regulating apoptosis, autophagy and the mitochondria‑associated membranes pathway.Experimental and Therapeutic Medicine,22,870.https://doi.org/10.3892/etm.2021.10302
Species: Rat
Tang,K.,Zhang,W.S.,Liu,Y.,Zhu,W.,& Qiu,J.(2021).Physicochemical properties and in vitro osteocompatibility of different titanium surfaces stored in a saline solution.Materials Research Express.
Species: Mouse
M00227-4 usage in article: APP:WB, SAMPLE:MC3T3-E1 CELL, DILUTION:NA
Huang X,Pei W,Ni B,Zhang R,You H.Chondroprotective and antiarthritic effects of Galangin in Osteoarthritis: An in Vitro and in Vivo Study.Eur J Pharmacol.2021 Jun 3:174232.doi:10.1016/j. ejphar.2021. 174232.Epub ahead of print.PMID:34090897.
Species: Rat
M00227-4 usage in article: APP:WB, SAMPLE:CHONDROCYTES, DILUTION:NA
Deng S,Zhang Y,Liao Z,Huang J,Huang R,Li Z.S100A4 plays a key role in TRPV3 ion channel expression and its electrophysiological function.Neurosci Lett.2021 May 28:135999.doi:10.1016/j.neulet.2021.135999.Epub ahead of print.PMID:34058292.
Species: Human
M00227-4 usage in article: APP:WB, SAMPLE:HEK293 T CELL, DILUTION:NA
Guo H,Zhao W,Liu A,Wu M,Shuai Y,Li B,Huang X,Liu X,Yang X,Guo X,Xuan K,Jin Y.SHED promote angiogenesis in stem cell-mediated dental pulp regeneration.Biochem Biophys Res Commun.2020 Sep 3;529(4):1158-1164.doi:10.1016/j.bbrc.2020.06.151.Epub 2020 Aug 3.PMID:32819580.
Species: Human,Pig
M00227-4 usage in article: APP:WB, SAMPLE:R-SHED AND SHED, DILUTION:NA
Chen M,Lu C,Lu H,Zhang J,Qin D,Liu S,Li X,Zhang L.Farnesoid X receptor via Notch1 directs asymmetric cell division of Sox9+ cells to prevent the development of liver cancer in a mouse model.Stem Cell Res Ther.2021 Apr 12;12(1):232.doi:10.1186/s13287-021-02298-6.PMID:33845903;PMCID:PMC8042944.
Species: Human,Mouse
M00227-4 usage in article: APP:WB, SAMPLE:LIVER TISSUE AND HUH7 CELL, DILUTION:NA
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-GAPDH Antibody
Question
My question regarding product M00227-4, anti-GAPDH antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
C. Rodriguez
Verified customer
Asked: 2020-04-17
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00227-4 anti-GAPDH antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-17
Question
I see that the anti-GAPDH antibody M00227-4 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-20
Question
Our lab want to know about using your anti-GAPDH antibody for gluconeogenesis studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-12-09
Answer
Thank you for your inquiry. This M00227-4 anti-GAPDH antibody is validated on hela cells. It is guaranteed to work for IF, IHC, WB in bovine, chicken, equine, human, mouse, pig, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-12-09
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for muscle using anti-GAPDH antibody M00227-4. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-21
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-21
Question
I was wanting to use your anti-GAPDH antibody for WB for pig muscle on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for pig muscle identification?
Verified Customer
Verified customer
Asked: 2019-08-05
Answer
You can see on the product datasheet, M00227-4 anti-GAPDH antibody has been tested for IF, IHC, WB on bovine, chicken, equine, human, mouse, pig, rat tissues. We have an innovator award program that if you test this antibody and show it works in pig muscle in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-05
Question
Here is the WB image, lot number and protocol we used for muscle using anti-GAPDH antibody M00227-4. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-11-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-27
Question
We are currently using anti-GAPDH antibody M00227-4 for chicken tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says bovine, chicken, equine, human, mouse, pig, rat. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2018-08-27
Answer
The anti-GAPDH antibody (M00227-4) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-08-27
Question
Do you have a BSA free version of anti-GAPDH antibody M00227-4 available?
Verified Customer
Verified customer
Asked: 2018-07-20
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GAPDH antibody M00227-4 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-07-20
Question
Does anti-GAPDH antibody M00227-4 work for WB with muscle?
G. Bhatt
Verified customer
Asked: 2018-01-16
Answer
According to the expression profile of muscle, GAPDH is highly expressed in muscle. So, it is likely that anti-GAPDH antibody M00227-4 will work for WB with muscle.
Boster Scientific Support
Answered: 2018-01-16
Question
Is this M00227-4 anti-GAPDH antibody reactive to the isotypes of GAPDH?
Verified Customer
Verified customer
Asked: 2017-11-06
Answer
The immunogen of M00227-4 anti-GAPDH antibody is Native porcine purified protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-11-06
Question
Our team were content with the WB result of your anti-GAPDH antibody. However we have observed positive staining in lung cytosol using this antibody. Is that expected? Could you tell me where is GAPDH supposed to be expressed?
H. Taylor
Verified customer
Asked: 2017-10-02
Answer
From what I have seen in literature, lung does express GAPDH. Generally GAPDH expresses in cytoplasm, cytosol. Regarding which tissues have GAPDH expression, here are a few articles citing expression in various tissues:
Astrocytoma, Pubmed ID: 10944468
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Heart, Pubmed ID: 7498159
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 2987855, 24275569
Lung, Pubmed ID: 3664468
Lymphoblast, Pubmed ID: 14654843
Muscle, Pubmed ID: 1193541, 11724794
Placenta, Pubmed ID: 1924305
Boster Scientific Support
Answered: 2017-10-02
Question
We bought anti-GAPDH antibody for IF on lung in a previous project. I am using bovine, and I plan to use the antibody for WB next. We want examining lung as well as colon adenocarcinoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2017-09-20
Answer
I looked at the website and datasheets of our anti-GAPDH antibody and it seems that M00227-4 has been tested on bovine in both IF and WB. Thus M00227-4 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in bovine even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-09-20
Question
We have been able to see staining in mouse colon adenocarcinoma. Do you have any suggestions? Is anti-GAPDH antibody supposed to stain colon adenocarcinoma positively?
R. Johnson
Verified customer
Asked: 2016-11-07
Answer
From literature colon adenocarcinoma does express GAPDH. From Uniprot.org, GAPDH is expressed in smooth muscle tissue, liver, lung, placenta, astrocytoma, leukemia, colon adenocarcinoma, eye, kidney, lung, lymph placenta, muscle, platelet, prostatic carcinoma, brain, cajal-retzius cell fetal brain cortex, heart, lymphoblast, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have GAPDH expression, here are a few articles citing expression in various tissues:
Astrocytoma, Pubmed ID: 10944468
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Heart, Pubmed ID: 7498159
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 2987855, 24275569
Lung, Pubmed ID: 3664468
Lymphoblast, Pubmed ID: 14654843
Muscle, Pubmed ID: 1193541, 11724794
Placenta, Pubmed ID: 1924305
Boster Scientific Support
Answered: 2016-11-07
Question
Will M00227-4 anti-GAPDH antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
M. Banerjee
Verified customer
Asked: 2015-07-17
Answer
As indicated on the product datasheet, M00227-4 anti-GAPDH antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-07-17
Question
Is a blocking peptide available for product anti-GAPDH antibody (M00227-4)?
L. Williams
Verified customer
Asked: 2014-12-24
Answer
We do provide the blocking peptide for product anti-GAPDH antibody (M00227-4). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-12-24
Question
I am interested in to test anti-GAPDH antibody M00227-4 on pig muscle for research purposes, then I may be interested in using anti-GAPDH antibody M00227-4 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
V. Jackson
Verified customer
Asked: 2014-02-12
Answer
The products we sell, including anti-GAPDH antibody M00227-4, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-02-12