Click image to see more details
Product Info Summary
| SKU: | PA1401 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Fractalkine CX3CL1 Antibody Picoband®
View all CX3CL1/Fractalkine Antibodies
SKU/Catalog Number
PA1401
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Fractalkine CX3CL1 Antibody catalog # PA1401. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Fractalkine CX3CL1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1401)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of mouse CX3CL1, identical to the related rat sequence.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1401 is reactive to Cx3cl1 in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
90-100 kDa
Calculated molecular weight
42099 MW
Background of CX3CL1/Fractalkine
Chemokine (C-X3-C motif) ligand 1, also known as CX3CL1, is a large cytokine protein. It is said to be the only known member of the CX3C chemokine family. It is also commonly known under the names fractalkine (in humans) and neurotactin (in mice). Its gene is mapped to the long arm of chromosome 8. Being the large protein, it has got 373 amino acids. Imai et al. (1997) identified a 7-transmembrane high-affinity receptor (CX3CR1; 601470) for fractalkine and showed that it mediates both the adhesive and migratory functions of fractalkine.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1401 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Mouse, Rat, -
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Positive Control
IHC: Rat Intestine Tissue
Validation Images & Assay Conditions
Click image to see more details
Anti-CX3CL1 antibody, PA1401, Western blotting
Lane 1: Recombinant Mouse Fractalkin Protein 10ng
Lane 2: Recombinant Mouse Fractalkin Protein 5ng
Lane 3: Recombinant Mouse Fractalkin Protein 2.5ng
Click image to see more details
Anti-CX3CL1 antibody, PA1401, IHC(P)
IHC(P): Rat Intestine Tissue
Protein Target Info & Infographic
Gene/Protein Information For Cx3cl1 (Source: Uniprot.org, NCBI)
Gene Name
Cx3cl1
Full Name
Fractalkine
Weight
42099 MW
Superfamily
intercrine delta family
Alternative Names
Fractalkine;C-X3-C motif chemokine 1;CX3C membrane-anchored chemokine;Neurotactin;Small-inducible cytokine D1;Processed fractalkine;Cx3cl1;Cx3c, Fkn, Scyd1; CX3CL1 ABCD-3, C3Xkine, CXC3, CXC3C, NTN, NTT, SCYD1, fractalkine, neurotactin C-X3-C motif chemokine ligand 1 fractalkine|C-X3-C motif chemokine 1|CX3C membrane-anchored chemokine|chemokine (C-X3-C motif) ligand 1|small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin)|small inducible cytokine subfamily D (Cys-X3-Cys), member-1|small-inducible cytokine D1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Cx3cl1, check out the Cx3cl1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Cx3cl1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Fractalkine CX3CL1 Antibody Picoband® (PA1401)
Hello CJ!
PA1401 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Yamada,S.,Miyoshi,S.,Nishio,J.,Mizutani,S.,Yamada,Z.,Kusunoki,N.,Sato,H.,Kuboi,Y.,Hoshino-Negishi,K.,Ishii,N.,Imai,T.,Mikami,T.,Nakano,H.,Kawai,S.,& Nanki,T.(2020).Effects of CX3CL1 inhibition on murine bleomycin-induced interstitial pneumonia.European Jo
Species: Mouse
PA1401 usage in article: APP:IHC, SAMPLE:LUNG TISSUE, DILUTION:NA
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Customer Reviews
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Customer Q&As
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17 Customer Q&As for Anti-Fractalkine CX3CL1 Antibody Picoband®
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for thoracic mammary gland using anti-CX3CL1 antibody PA1401. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-26
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-26
Question
I am looking for using your anti-CX3CL1 antibody for positive regulation of angiogenesis studies. Has this antibody been tested with western blotting on rat intestine tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
We appreciate your inquiry. This PA1401 anti-CX3CL1 antibody is validated on rat intestine tissue. It is guaranteed to work for IHC, WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-02-28
Question
We have observed staining in rat cerebrospinal fluid. What should we do? Is anti-CX3CL1 antibody supposed to stain cerebrospinal fluid positively?
Verified Customer
Verified customer
Asked: 2019-11-18
Answer
From what I have seen in literature cerebrospinal fluid does express CX3CL1. From what I have seen in Uniprot.org, CX3CL1 is expressed in thoracic mammary gland, brain, cerebrospinal fluid, among other tissues. Regarding which tissues have CX3CL1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Cerebrospinal fluid, Pubmed ID: 19838169
Boster Scientific Support
Answered: 2019-11-18
Question
I was wanting to use your anti-CX3CL1 antibody for IHC for mouse thoracic mammary gland on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse thoracic mammary gland identification?
E. Williams
Verified customer
Asked: 2019-10-28
Answer
You can see on the product datasheet, PA1401 anti-CX3CL1 antibody has been tested for IHC, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse thoracic mammary gland in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-28
Question
Is there a BSA free version of anti-CX3CL1 antibody PA1401 available?
Verified Customer
Verified customer
Asked: 2019-10-14
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CX3CL1 antibody PA1401 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-14
Question
Our lab used your anti-CX3CL1 antibody for WB on thoracic mammary gland in a previous experiment. I am using rat, and We are going to use the antibody for IHC next. We need examining thoracic mammary gland as well as brain in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2019-10-03
Answer
I took a look at the website and datasheets of our anti-CX3CL1 antibody and I see that PA1401 has been tested on rat in both WB and IHC. Thus PA1401 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-10-03
Question
We are currently using anti-CX3CL1 antibody PA1401 for rat tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
The anti-CX3CL1 antibody (PA1401) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-26
Question
I see that the anti-CX3CL1 antibody PA1401 works with IHC, what is the protocol used to produce the result images on the product page?
L. Zhang
Verified customer
Asked: 2019-06-05
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-05
Question
Is this PA1401 anti-CX3CL1 antibody reactive to the isotypes of CX3CL1?
Verified Customer
Verified customer
Asked: 2019-04-10
Answer
The immunogen of PA1401 anti-CX3CL1 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of mouse CX3CL1(74-92aa CADPKEKWVQDAMKHLDHQ), identical to the related rat sequence. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-04-10
Question
We were content with the WB result of your anti-CX3CL1 antibody. However we have seen positive staining in brain cell membrane using this antibody. Is that expected? Could you tell me where is CX3CL1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-11-26
Answer
From literature, brain does express CX3CL1. Generally CX3CL1 expresses in cell membrane. Regarding which tissues have CX3CL1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Cerebrospinal fluid, Pubmed ID: 19838169
Boster Scientific Support
Answered: 2018-11-26
Question
I have a question about product PA1401, anti-CX3CL1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
S. Mangal
Verified customer
Asked: 2018-07-10
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1401 anti-CX3CL1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-07-10
Question
Does PA1401 anti-CX3CL1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-05-10
Answer
As indicated on the product datasheet, PA1401 anti-CX3CL1 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-05-10
Question
Is a blocking peptide available for product anti-CX3CL1 antibody (PA1401)?
Verified Customer
Verified customer
Asked: 2018-04-11
Answer
We do provide the blocking peptide for product anti-CX3CL1 antibody (PA1401). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-04-11
Question
Will anti-CX3CL1 antibody PA1401 work for IHC with thoracic mammary gland?
Verified Customer
Verified customer
Asked: 2017-12-01
Answer
According to the expression profile of thoracic mammary gland, CX3CL1 is highly expressed in thoracic mammary gland. So, it is likely that anti-CX3CL1 antibody PA1401 will work for IHC with thoracic mammary gland.
Boster Scientific Support
Answered: 2017-12-01
Question
I have attached the WB image, lot number and protocol we used for thoracic mammary gland using anti-CX3CL1 antibody PA1401. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-09-11
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-09-11
Question
We are interested in to test anti-CX3CL1 antibody PA1401 on mouse thoracic mammary gland for research purposes, then I may be interested in using anti-CX3CL1 antibody PA1401 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-07-24
Answer
The products we sell, including anti-CX3CL1 antibody PA1401, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-07-24
Question
Will anti-CX3CL1 antibody PA1401 work on canine WB with thoracic mammary gland?
J. Collins
Verified customer
Asked: 2014-04-22
Answer
Our lab technicians have not validated anti-CX3CL1 antibody PA1401 on canine. You can run a BLAST between canine and the immunogen sequence of anti-CX3CL1 antibody PA1401 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine thoracic mammary gland in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-04-22
