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Product Info Summary
| SKU: | EK0762 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human Galectin-1 ELISA Kit PicoKine®
View all Galectin-1 ELISA kits
SKU/Catalog Number
EK0762
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Galectin-1 ELISA Kit PicoKine® (96 Tests). Quantitate Human LGALS1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Galectin-1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0762)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: A2-D135
Sensitivity
<15 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0762 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0762 is reactive to LGALS1 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0762 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Galectin-1
Galectin-1 is a protein that in humans is encoded by the LGALS1 gene. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-1 may act as an autocrine negative growth factor that regulates cell proliferation. Its expression in Hodgkin Lymphoma has also been shown to mediate immunosuppression of CD8+ T-cells. Additionally, Galectin-1 is thought to play a role in creating immune tolerance in pregnancy.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0762-CAP | Anti-Human LGALS1 Pre-coated 96-well strip microplate | 1 |
| EK0762-ST | Human LGALS1 Standard | 2 vials, 10 ng/tube |
| EK0762-DA | Human LGALS1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.047 | 0.119 | 0.144 | 0.252 | 0.516 | 0.892 | 1.609 | 2.092 |
Data Example Images
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Human Galectin-1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Galectin-1, Dilution ratio of 1:1, concentration in serum and plasma is around 4 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Galectin-1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 232 | 1786 | 5647 | 229 | 1822 | 5807 |
| Standard deviation | 13.45 | 121.44 | 355.76 | 13.96 | 147.58 | 476.17 |
| CV (%) | 5.8% | 6.8% | 8.1% | 6.1% | 8.1% | 8.2% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Galectin-1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 232 | 226 | 230 | 221 | 227 | 4.2 | 1.8% |
| Sample 2 | 1786 | 1592 | 1746 | 1838 | 1740 | 91.73 | 5.2% |
| Sample 3 | 5647 | 5665 | 6073 | 5299 | 5671 | 274.13 | 4.8% |
Protein Target Info & Infographic
Gene/Protein Information For LGALS1 (Source: Uniprot.Org, NCBI)
Gene Name
LGALS1
Full Name
Galectin-1
Weight
14716 MW
Alternative Names
Galectin-1;Gal-1;14 kDa laminin-binding protein;HLBP14;14 kDa lectin;Beta-galactoside-binding lectin L-14-I;Galaptin;HBL;HPL;Lactose-binding lectin 1;Lectin galactoside-binding soluble 1;Putative MAPK-activating protein PM12;S-Lac lectin 1;LGALS1; LGALS1 GAL1, GBP galectin 1 galectin-1|14 kDa laminin-binding protein|14 kDa lectin|HBL|HLBP14|HPL|S-Lac lectin 1|beta-galactoside-binding lectin L-14-I|beta-galactoside-binding protein 14kDa|epididymis secretory sperm binding protein|gal-1|galaptin|lactose-binding lectin 1|lectin, galactoside-binding, soluble, 1|putative MAPK-activating protein PM12
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on LGALS1, check out the LGALS1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for LGALS1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Galectin-1 ELISA Kit PicoKine® (EK0762)
Hello CJ!
EK0762 has been cited in 17 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Autocrine Signaling of NRP1 Ligand Galectin-1 Elicits Resistance to BRAF-Targeted Therapy in Melanoma Cells
Galectin-1 as a predictive biomarker in ovarian cancer
Serum endocan and endothelial dysfunction in childhood acute lymphoblastic leukemia survivors: a tertiary center experience:
Endocan as a Potential Biomarker of Disease Severity and Exacerbations in COPD
Endocan as a marker of disease severity in pulmonary thromboembolism
Maternal serum galectin-1 and galectin-3 levels in pregnancies complicated with preterm prelabor rupture of membranes
Cerebrospinal Fluid Galectin-1 Levels Discriminate Patients with Parkinsonism from Controls
Galectin-1 and Galectin-3 Levels in Patients with Schizophrenia and their Unaffected Siblings
A “Bridge-Building” Glycan Scaffold Mimicking Microbial Invasion for In Situ Endothelialization
A “Bridge-Building” Glycan Scaffold Mimicking Microbial Invasion for In Situ Endothelialization
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Customer Reviews
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1 Reviews For Human Galectin-1 ELISA Kit PicoKine®
0
Works Well For Detection Of Gal-1 Over A Broad Range Of Concentration--Mahesh Raundhal, Weill Cornell Medicine, Medicine, Postdoctoral Fellow
Excellent
Source: Biocompare.com
| Applications | ELISA |
|---|---|
| Sample | Human ovarian cancer ascites |
| Detection | -- |
"Human ovarian cancer ascites samples were tested for presence of Galectin-1 protein. Depending on the donor, ascites samples were found to have varying concentrations of Gal-1. Straightforward and easy to use once a general idea of Gal-1 concentration in sample of interest is optimized." (Customer’s Tip: "If using for the first time, plate samples at least 3-4 dilutions to get consistent results among samples at the same dilution.")
Mahesh Raundhal
Verified customer
Submitted 2017-08-10
Customer Q&As
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Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
9 Customer Q&As for Human Galectin-1 ELISA Kit PicoKine®
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of Galectin-1 in Human cell lysates? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2020-09-23
Answer
A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Galectin-1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-09-23
Question
Does EK0762 react with mouse?
Verified customer
Asked: 2020-06-23
Answer
Our lab tested recombinant mouse Galectin-1 protein using EK0762 Human Galectin-1 ELISA Kit and approximately 9.1% cross reactivity was observed.
Boster Scientific Support
Answered: 2020-06-24
Question
Q: which procedure should I follow in order to thaw whole blood sample for Galectin-1 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2020-03-15
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Galectin-1 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2020-03-15
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2019-12-25
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-12-25
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
A. Anand
Verified customer
Asked: 2019-11-11
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-11-11
Question
Q: What is the optimal O.D. value for Galectin-1 ELISA kit? I used your Galectin-1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Galectin-1 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2018-02-23
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Galectin-1 ELISA kit to have sensitivity of 15pg/ml, that means the minimum amount of Galectin-1 that can be declared/interpreted as positive by the above standard is 15pg/ml.
Boster Scientific Support
Answered: 2018-02-23
Question
i want to buy human galectin 1 elisa kit but my query is that can it work for serum samples that are approximately 14 months old.
Verified Customer
Verified customer
Asked: 2017-07-26
Answer
We can not guarantee the kit will work for this long storaged serum samples. We suggest the customer run a pilot test first.
Boster Scientific Support
Answered: 2017-07-26
Question
Q: can I use citrate plasma as samples in Human Galectin-1 Picokine® ELISA Kit (Catalog # EK0762)?
H. Miller
Verified customer
Asked: 2016-09-19
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of Galectin-1 causing degradation of its protein structure. Galectin-1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the Galectin-1 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2016-09-19
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
T. Nelson
Verified customer
Asked: 2016-01-22
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2016-01-22
