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Product Info Summary
| SKU: | M00364 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | ELISA, IHC |
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Product info
Product Name
Anti-Elafin/Skalp PI3 Antibody (monoclonal, C7)
View all Trappin-2/Elafin/Skalp Antibodies
SKU/Catalog Number
M00364
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Elafin/Skalp PI3 Antibody (monoclonal, C7) catalog # M00364. Tested in ELISA, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Elafin/Skalp PI3 Antibody (monoclonal, C7) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00364)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
C7
Isotype
Mouse IgG1
Immunogen
E. coli-derived human Elafin/Skalp recombinant protein (Position: A61-Q117).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M00364 is reactive to PI3 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
25 kDa
Calculated molecular weight
12.27kDa
Background of Trappin-2/Elafin/Skalp
Elafin, also known as peptidase inhibitor 3 or skin-derived antileukoprotease (SKALP), is a protein that in humans is encoded by the PI3 gene. This gene encodes an elastase-specific inhibitor that functions as an antimicrobial peptide against Gram-positive and Gram-negative bacteria, and fungal pathogens. The protein contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. And this gene belongs to the centromeric cluster. Expression of this gene is upgulated by bacterial lipopolysaccharides and cytokines.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00364 is guaranteed for ELISA, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
ELISA (Cap), 1-5μg/ml
Positive Control
IHC: human tonsil tissue
Validation Images & Assay Conditions
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Figure 1. IHC analysis of Elafin using anti-Elafin antibody (M00364).
Elafin was detected in paraffin-embedded section of human tonsil tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Elafin Antibody (M00364) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
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Figure 2. Sandwich ELISA - Recombinant human Elafin/Skalp protein standard curve.
Use in combination with reagents from Human Elafin/Skalp ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ1117).
Protein Target Info & Infographic
Gene/Protein Information For PI3 (Source: Uniprot.org, NCBI)
Gene Name
PI3
Full Name
Elafin
Weight
12.27kDa
Alternative Names
Elafin; Elastase-specific inhibitor; ESI; Peptidase inhibitor 3; PI-3; Protease inhibitor WAP3; Skin-derived antileukoproteinase; SKALP; WAP four-disulfide core domain protein 14; PI3; WAP3; WFDC14 PI3 ESI, SKALP, WAP3, WFDC14, cementoin peptidase inhibitor 3 elafin|PI-3|WAP four-disulfide core domain 14|WAP four-disulfide core domain protein 14|elastase-specific inhibitor|peptidase inhibitor 3, skin-derived|pre-elafin|protease inhibitor 3, skin-derived (SKALP)|protease inhibitor WAP3|skin-derived antileukoproteinase|trappin-2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PI3, check out the PI3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PI3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Elafin/Skalp PI3 Antibody (monoclonal, C7) (M00364)
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