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Product Info Summary
| SKU: | EK0358 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Rat |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Rat Fractalkine/CX3CL1 ELISA Kit PicoKine®
View all CX3CL1/Fractalkine ELISA kits
SKU/Catalog Number
EK0358
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rat Fractalkine/CX3CL1 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Cx3cl1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat Fractalkine/CX3CL1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0358)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: Q25-T334
Sensitivity
<10 pg/ml
Assay Range
312 pg/ml - 20,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0358 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0358 is reactive to Cx3cl1 in Rat samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK0358 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CX3CL1/Fractalkine
Chemokine (C-X3-C motif) ligand 1 (CX3CL1) is a large cytokine protein of 373 amino acids, it contains multiple domains and is the only known member of the CX3C chemokine family. It is also commonly known under the names fractalkine (in humans) and neurotactin (in mice). CX3CL1 is produced as a long protein (with 373-amino acid in humans) with an extended mucin-like stalk and a chemokine domain on top. The mucin-like stalk permits it to bind to the surface of certain cells. However a soluble (90 kD) version of this chemokine has also been observed. Soluble CX3CL1 potently chemoattracts T cells and monocytes, while the cell-bound chemokine promotes strong adhesion of leukocytes to activated endothelial cells, where it is primarily expressed. CX3CL1 elicits its adhesive and migratory functions by interacting with the chemokine receptor CX3CR1. Its gene is located on human chromosome 16 along with some CC chemokines known as CCL17 and CCL22. It can act as a mediator of smooth muscle cell migration in human atherosclerosis, rather than mediate inflammatory cell recruitment.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0358-CAP | Anti-Rat Cx3cl1 Pre-coated 96-well strip microplate | 1 |
| EK0358-ST | Rat Cx3cl1 Standard | 2 vials, 20 ng/tube |
| EK0358-DA | Rat Cx3cl1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
| O.D. | 0.003 | 0.071 | 0.148 | 0.276 | 0.497 | 0.927 | 1.440 | 1.895 |
Data Example Images
Click image to see more details
Rat Fractalkine PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat Fractalkine/CX3CL1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 448 | 1815 | 9967 | 464 | 1947 | 10491 |
| Standard deviation | 32.7 | 76.23 | 687.72 | 35.72 | 87.61 | 912.71 |
| CV (%) | 7.3% | 4.2% | 6.9% | 7.7% | 4.5% | 8.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CX3CL1/Fractalkine in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 448 | 452 | 401 | 450 | 437 | 21.26 | 4.8% |
| Sample 2 | 1815 | 1598 | 1565 | 1811 | 1697 | 116.34 | 6.8% |
| Sample 3 | 9967 | 9196 | 9761 | 9169 | 9523 | 348.57 | 3.6% |
Protein Target Info & Infographic
Gene/Protein Information For Cx3cl1 (Source: Uniprot.Org, NCBI)
Gene Name
Cx3cl1
Full Name
Fractalkine
Weight
41977 MW
Superfamily
intercrine delta family
Alternative Names
Fractalkine;C-X3-C motif chemokine 1;CX3C membrane-anchored chemokine;Neurotactin;Small-inducible cytokine D1;Processed fractalkine;Cx3cl1;Acc1, Fkn, Scyd1; CX3CL1 ABCD-3, C3Xkine, CXC3, CXC3C, NTN, NTT, SCYD1, fractalkine, neurotactin C-X3-C motif chemokine ligand 1 fractalkine|C-X3-C motif chemokine 1|CX3C membrane-anchored chemokine|chemokine (C-X3-C motif) ligand 1|small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin)|small inducible cytokine subfamily D (Cys-X3-Cys), member-1|small-inducible cytokine D1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Cx3cl1, check out the Cx3cl1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Cx3cl1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Rat Fractalkine/CX3CL1 ELISA Kit PicoKine® (EK0358)
Hello CJ!
EK0358 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhao, B., He, E., Pan, Y., Xu, H., & Song, X. (2017). Kindlin-1 is a key protein in hyperbaric oxygen therapy for the treatment of neuropathic pain. Molecular Pain, 13, 1-10. doi: 10.1177/1744806917730254
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Customer Reviews
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Customer Q&As
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9 Customer Q&As for Rat Fractalkine/CX3CL1 ELISA Kit PicoKine®
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2020-12-14
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-12-14
Question
Q: how to analyze ELISA data? I have obtained CX3CL1 level in serum.
Y. Martin
Verified customer
Asked: 2020-04-05
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-04-05
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-11-25
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-11-25
Question
Q: how can I thaw whole blood sample for CX3CL1 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2019-10-26
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CX3CL1 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2019-10-26
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
J. Singh
Verified customer
Asked: 2018-07-22
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-07-22
Question
Q: if the enzyme conjugated CX3CL1 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CX3CL1 antigen?
Verified Customer
Verified customer
Asked: 2018-05-05
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-05-05
Question
Q: can you recommend the dilution ratio of serum samples for detection of CX3CL1 in Rat serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.
S. Hall
Verified customer
Asked: 2018-03-11
Answer
A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CX3CL1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-03-11
Question
Q: can I use citrate plasma as samples in Rat CX3CL1 Picokine® ELISA Kit (Catalog # EK0358)?
K. Roberts
Verified customer
Asked: 2018-01-26
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of CX3CL1 causing disruption of its protein structure. CX3CL1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the CX3CL1 ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-01-26
Question
Q: What is the optimal O.D. value for CX3CL1 ELISA kit? I performed your CX3CL1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain CX3CL1 even though the O.D. values are not very high?
H. Walker
Verified customer
Asked: 2017-04-14
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CX3CL1 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of CX3CL1 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2017-04-14
