Anti-Estrogen Receptor Alpha ESR1 Antibody (Monoclonal, C-542)

Product Name

Anti-Estrogen Receptor Alpha ESR1 Antibody (Monoclonal, C-542)

View all ER alpha/NR3A1 Antibodies

SKU/Catalog Number

M00057

Size

200μg/vial

Form

Liquid

Description

Boster Bio Anti-Estrogen Receptor Alpha ESR1 Antibody (Monoclonal, C-542) (Catalog# M00057). Tested in ICC, IHC, IP, WB application(s). This antibody reacts with Human, Mouse, Rat, Hamster.

Storage & Handling

Store at -20°C for long-term storage. Avoid freeze/thaw cycles.

Cite This Product

Anti-Estrogen Receptor Alpha ESR1 Antibody (Monoclonal, C-542) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00057)

Host

Mouse

Contents

Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.

Clonality

Monoclonal

Clone Number

C-542

Isotype

IgG1

Immunogen

Synthetic peptide corresponding to the sequence near the C-terminus of human Estrogen Receptor α

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00057 is reactive to ESR1 in Hamster, Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

66216 MW

Background of ER alpha/NR3A1

The estrogen receptor alpha (ERalpha, UniProt/Swiss-Prot entry P03372) belongs to the family of nuclear hormone receptors, which are ligand-activated transcription factors. They are important for the regulation of gene expression, cellular proliferation and differentiation, sexual development and reproductive function. Estrogen receptors are also involved in pathological processes such as breast cancer, and osteoporosis. ERalpha can regulate transcription by direct binding to estrogen response elements (EREs) in the DNA or by interaction with other transcription factors. It may also form a heterodimer with ERbeta.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00057 is guaranteed for IP, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western Blot (1:500, colorimetric). Detects a band of 66.3kDa.
Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Validation Images & Assay Conditions

Gene/Protein Information For ESR1 (Source: Uniprot.org, NCBI)

Alternative Names

Estrogen receptor;ER;ER-alpha;Estradiol receptor;Nuclear receptor subfamily 3 group A member 1;ESR1;ESR, NR3A1; ESR1 ER, ESR, ESRA, ESTRR, Era, NR3A1 estrogen receptor 1 estrogen receptor|E2 receptor alpha|ER-alpha|estradiol receptor|estrogen nuclear receptor alpha|estrogen receptor alpha E1-E2-1-2|estrogen receptor alpha E1-N2-E2-1-2|nuclear receptor subfamily 3 group A member 1|oestrogen receptor alpha

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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