Anti-CLU Antibody (N-term)

Product Name

Anti-CLU Antibody (N-term)

View all Clusterin/APOJ Antibodies

SKU/Catalog Number

A00590-2

Size

80 µl

Form

Liquid

Description

Boster Bio Anti-CLU Antibody (N-term) (Catalog # A00590-2). Tested in WB, Flow Cytometry, IHC-P-Leica application(s). This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-CLU Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00590-2)

Host

Rabbit

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

This CLU antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 71-99 amino acids from the N-terminal region of human CLU.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A00590-2 is reactive to CLU in Human, Mouse, Rat

Applications

A00590-2 is guaranteed for Flow Cytometry, WB, IHC-P-Leica Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

52495 Da

Background of Clusterin/APOJ

The protein encoded by this gene appears to be involved in several basic biological events such as cell death, tumor progression, and neurodegenerative disorders. However, the function of this protein is unknown. Three transcript variants encoding different isoforms have been found for this gene, and one of them is secreted and processed into a mature form.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500-1000
IHC-P-Leica: 1:500
FC: 1:25

Validation Images & Assay Conditions

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All lanes : Anti-CLU Antibody (N-term) at 1:1000 dilution
Lane 1: HepG2 whole cell lysate
Lane 2: MCF-7 whole cell lysate
Lane 3: Caco2 whole cell lysate
Lane 4: Hela whole cell lysate
Lane 5: A549 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 52 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

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Western blot analysis of lysates from Hela cell line, human liver and human testis tissue lysate(from left to right), using CLU Antibody (N-term). A00590-2 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

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Western blot analysis of lysates from A549 cell line and human plasma tissue lysate(from left to right), using CLU Antibody (N-term). A00590-2 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

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Immunohistochemical analysis of paraffin-embedded human pancreas tissue using A00590-2 performed on the Leica® BOND RXm. Samples were incubated with primary antibody(1/500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

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Overlay histogram showing Hela cells stained with A00590-2 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (A00590-2, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Gene/Protein Information For CLU (Source: Uniprot.org, NCBI)

Alternative Names

40; 40, sulfated glycoprotein 2; Aging-associated gene 4 protein; aging-associated protein 4; APOJ; apo-J; Apolipoprotein J; CLI; CLIclusterin (complement lysis inhibitor, SP-40; CLU; Clusterin; Complement cytolysis inhibitor; complement lysis inhibitor; Complement-associated protein SP-40; Ku70-binding protein 1; KUB1SGP2; MGC24903; NA1/NA2; SGP-2; SP-40; sulfated glycoprotein 2; Testosterone-repressed prostate message 2; testosterone-repressed prostate message 2, apolipoprotein J); TRPM-2; TRPM-2TRPM2 CLU AAG4, APO-J, APOJ, CLI1, CLU2, KUB1, NA1/NA2, SGP-2, SGP2, SP-40, TRPM-2, TRPM2, CLU clusterin clusterin|aging-associated protein 4|apolipoprotein J|complement cytolysis inhibitor|complement lysis inhibitor|complement-associated protein SP-40,40|epididymis secretory sperm binding protein|ku70-binding protein 1|sulfated glycoprotein 2|testosterone-repressed prostate message 2

For more info on CLU, check out the CLU Infographic

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In this infographic, you will see the following information for CLU: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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