Anti-Esrp-1/2 Esrp2 Monoclonal Antibody

Product Name

Anti-Esrp-1/2 Esrp2 Monoclonal Antibody

View all Esrp2 Antibodies

SKU/Catalog Number

M06068-4

Size

25uL

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Esrp-1/2 Esrp2 Monoclonal Antibody (Catalog # M06068-4). Tested in WB applications. This antibody reacts with Mouse.

Storage & Handling

Store vial at -20°C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. Expiration date is three (3) months from date of opening. (Ship on dry ice.)

Cite This Product

Anti-Esrp-1/2 Esrp2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06068-4)

Host

Mouse

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Monoclonal

Clone Number

Clone: 23A7.C9

Isotype

IgG2b kappa

Immunogen

Anti-Esrp-1/2 was produced by repeated immunizations of full length recombinant mouse Esrp-1 fusion protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M06068-4 is reactive to Esrp2 in Mouse

Applications

M06068-4 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

107413 MW

Background of Esrp2

Epithelial splicing regulatory protein 1 (Esrp-1) and Esrp-2 are mRNA splicing factors that regulate the formation of epithelial cell-specific isoforms. They specifically regulate the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2, and also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Esrp-1 and -2 act by directly binding specific sequences in mRNAs. They bind the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 2.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:750,000
WB: 1:1000

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Esrp1, Esrp2 using anti-Esrp1, Esrp2 antibody (M06068-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Esrp1, Esrp2 antigen affinity purified polyclonal antibody (Catalog # M06068-4) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1021) with Tanon 5200 system. A specific band was detected for Esrp1, Esrp2.

Gene/Protein Information For Esrp2 (Source: Uniprot.org, NCBI)

Alternative Names

epithelial splicing regulatory protein 2; FLJ21918; FLJ22248; RBM35BRNA-binding protein 35B; RNA binding motif protein 35A; RNA binding motif protein 35B; RNA-binding motif protein 35B Esrp2|9530027K23Rik, Rbm35, Rbm35b|epithelial splicing regulatory protein 2|epithelial splicing regulatory protein 2|RNA binding motif protein 35b|RNA-binding motif protein 35B|RNA-binding protein 35B

For more info on Esrp2, check out the Esrp2 Infographic

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In this infographic, you will see the following information for Esrp2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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