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Product Info Summary
| SKU: | A08004 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-RBM35B ESRP2 Antibody
SKU/Catalog Number
A08004
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-RBM35B ESRP2 Antibody (Catalog # A08004). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
RBM35B antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-RBM35B ESRP2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08004)
Host
Rabbit
Contents
RBM35B Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
RBM35B antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human RBM35B. The immunogen is located within the last 50 amino acids of RBM35B.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
RBM35B antibody is predicted to not cross-react with other RBM35/ESRP family members. At least two isoforms of RBM35B are known to exist; this antibody will detect both isoforms.
Reactive Species
A08004 is reactive to Esrp2 in Human, Mouse
Applications
A08004 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
78401 MW
Background of Esrp2
RBM35A, also known as ESRP2, is a mRNA splicing factor that with its related protein RBM35A (ESRP1) are coordinators of an epithelial cell-type-specific splicing program. Both RBM35B and RBM35A are involved in posttranscriptional regulation of a number of genes such as FGFR2, CD44, CTNND1, and ENAH by exerting a differential effect on protein translation via 5' UTRs of mRNAs, suggesting that these proteins are global regulators of an epithelial regulatory network. Loss of this global ESRP-regulated epithelial splicing programme induces the phenotypic changes in cell morphology that are observed during the epithelial-mesenchymal transition.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
RBM35B antibody can be used for detection of RBM35B by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of RBM35B in human lung tissue lysate with RBM35B antibody at 1 μg/mL.
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Immunohistochemistry of RBM35B in human lung tissue with RBM35B antibody at 5 μg/mL.
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Immunofluorescence of RBM35B in human lung tissue cells with RBM35B antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For Esrp2 (Source: Uniprot.org, NCBI)
Gene Name
Esrp2
Full Name
Epithelial splicing regulatory protein 2
Weight
78401 MW
Superfamily
ESRP family
Alternative Names
epithelial splicing regulatory protein 2; FLJ21918; FLJ22248; RBM35BRNA-binding protein 35B; RNA binding motif protein 35A; RNA binding motif protein 35B; RNA-binding motif protein 35B Esrp2|9530027K23Rik, Rbm35, Rbm35b|epithelial splicing regulatory protein 2|epithelial splicing regulatory protein 2|RNA binding motif protein 35b|RNA-binding motif protein 35B|RNA-binding protein 35B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Esrp2, check out the Esrp2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Esrp2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RBM35B ESRP2 Antibody (A08004)
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