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Product Info Summary
| SKU: | A06068 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-RBM35A ESRP1 Antibody
SKU/Catalog Number
A06068
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-RBM35A ESRP1 Antibody (Catalog # A06068). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
RBM35A antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-RBM35A ESRP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06068)
Host
Rabbit
Contents
RBM35A Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
RBM35A antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of human RBM35A. The immunogen is located within amino acids 30 - 80 of RBM35A.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
RBM35A antibody is predicted to not cross-react with other RBM35/ESRP family members. At least five isoforms of RBM35A are known to exist; this antibody will detect all five.
Reactive Species
A06068 is reactive to ESRP1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
75585 MW
Background of Esrp1
RBM35A, also known as ESRP1, is a mRNA splicing factor that with its related protein RBM35B (ESRP2) are coordinators of an epithelial cell-type-specific splicing program. RBM35A contains three putative RNA recognition motifs and acts by directly binding specific sequences in mRNAs. RBM35A is involved in posttranscriptional regulation of a number of genes such as FGFR2, CD44, CTNND1, and ENAH by exerting a differential effect on protein translation via 5' UTRs of mRNAs. Other recent studies have shown that RMB35A may also act as a novel tumor suppressor.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06068 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
RBM35A antibody can be used for detection of RBM35A by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of RBM35A in rat colon tissue lysate with RBM35A antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of RBM35A in human colon tissue with RBM35A antibody at 2.5 μg/mL.
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Immunofluorescence of RBM35A in human colon tissue cells with RBM35A antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For ESRP1 (Source: Uniprot.org, NCBI)
Gene Name
ESRP1
Full Name
Epithelial splicing regulatory protein 1
Weight
75585 MW
Superfamily
ESRP family
Alternative Names
RBM35A, RMB35A, RBM35A, Epithelial splicing regulatory protein 1, RNA-binding motif protein 35A ESRP1 DFNB109, RBM35A, RMB35A epithelial splicing regulatory protein 1 epithelial splicing regulatory protein 1|RNA-binding motif protein 35A|RNA-binding protein 35A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ESRP1, check out the ESRP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ESRP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RBM35A ESRP1 Antibody (A06068)
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