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Product Info Summary
| SKU: | A01825 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC |
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Product info
Product Name
Anti-JunB (Ab-79) Antibody
SKU/Catalog Number
A01825
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-JunB (Ab-79) Antibody (Catalog # A01825). Tested in IHC, IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-JunB (Ab-79) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01825)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa.77~81 (G-A-S-L-K) derived from Human JunB.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A01825 is reactive to JUNB in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
17935 MW
Background of JunB/AP-1
Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'.
Narayanan K, et al. (2004) J Biol Chem. 279(43): 44294-442302.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01825 is guaranteed for IF, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 43kd
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Validation Images & Assay Conditions
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Figure 1. IHC analysis of JUNB using anti-JUNB antibody (A01825).
JUNB was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-JUNB Antibody (A01825) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Immunofluorescence staining of methanol-fixed Hela cells using JunB(Ab-79) Antibody #A01825.
Protein Target Info & Infographic
Gene/Protein Information For JUNB (Source: Uniprot.org, NCBI)
Gene Name
JUNB
Full Name
Transcription factor jun-B
Weight
17935 MW
Superfamily
bZIP family
Alternative Names
Thy-1 membrane glycoprotein;CDw90;Thy-1 antigen;CD90;THY1; JUNB AP-1 JunB proto-oncogene, AP-1 transcription factor subunit transcription factor jun-B|activator protein 1|jun B proto-oncogene
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on JUNB, check out the JUNB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for JUNB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-JunB (Ab-79) Antibody (A01825)
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