Anti-Esrp-1 Monoclonal Antibody

Product Name

Anti-Esrp-1 Monoclonal Antibody

View all Esrp1 Antibodies

SKU/Catalog Number

M06068-1

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Esrp-1 Monoclonal Antibody (Catalog # M06068-1). Tested in WB applications. This antibody reacts with Mouse.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-Esrp-1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06068-1)

Host

Mouse

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Monoclonal

Clone Number

Clone: 27H12.F3.H5

Isotype

IgG2b

Immunogen

Anti-Esrp-1 was produced by repeated immunizations of full length recombinant mouse Esrp-1 fusion protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M06068-1 is reactive to ESRP1 in Mouse

Applications

M06068-1 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

75585 MW

Background of Esrp1

Epithelial splicing regulatory protein 1 (Esrp-1) is an mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. It specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2, and also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). EsrpP-1 acts by directly binding specific sequences in mRNAs. It binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:750,000
WB: 1:1000

Validation Images & Assay Conditions

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Western blot analysis of ESRP1 expression in GFP-transfected 293T cell lysates (lane 1), ESRP2-transfected 293T cell lysates (lane 3) and transfected 293T cell extracts (lane 2). ESRP1 was detected using mouse anti-ESRP1 monoclonal antibody (Catalog # M06068-1) at 1:1000. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1001).

Gene/Protein Information For ESRP1 (Source: Uniprot.org, NCBI)

Alternative Names

epithelial splicing regulatory protein 1; FLJ20171; RBM35A; RMB35A; RNA binding motif protein 35A; RNA-binding motif protein 35A; RNA-binding protein 35A ESRP1 DFNB109, RBM35A, RMB35A epithelial splicing regulatory protein 1 epithelial splicing regulatory protein 1|RNA-binding motif protein 35A|RNA-binding protein 35A

For more info on ESRP1, check out the ESRP1 Infographic

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In this infographic, you will see the following information for ESRP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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