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Product Info Summary
| SKU: | M06068-1 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Mouse |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-Esrp-1 Monoclonal Antibody
SKU/Catalog Number
M06068-1
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-Esrp-1 Monoclonal Antibody (Catalog # M06068-1). Tested in WB applications. This antibody reacts with Mouse.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-Esrp-1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06068-1)
Host
Mouse
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Monoclonal
Clone Number
Clone: 27H12.F3.H5
Isotype
IgG2b
Immunogen
Anti-Esrp-1 was produced by repeated immunizations of full length recombinant mouse Esrp-1 fusion protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M06068-1 is reactive to ESRP1 in Mouse
Reconstitution
Calculated molecular weight
75585 MW
Background of Esrp1
Epithelial splicing regulatory protein 1 (Esrp-1) is an mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. It specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2, and also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). EsrpP-1 acts by directly binding specific sequences in mRNAs. It binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06068-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:750,000
WB: 1:1000
Validation Images & Assay Conditions
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Western blot analysis of ESRP1 expression in GFP-transfected 293T cell lysates (lane 1), ESRP2-transfected 293T cell lysates (lane 3) and transfected 293T cell extracts (lane 2). ESRP1 was detected using mouse anti-ESRP1 monoclonal antibody (Catalog # M06068-1) at 1:1000. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1001).
Protein Target Info & Infographic
Gene/Protein Information For ESRP1 (Source: Uniprot.org, NCBI)
Gene Name
ESRP1
Full Name
Epithelial splicing regulatory protein 1
Weight
75585 MW
Superfamily
ESRP family
Alternative Names
Esrp-1, Esrp1, Rbm35a, Epithelial splicing regulatory protein 1, RNA-binding protein 35A, RNA-binding motif protein 35A ESRP1 DFNB109, RBM35A, RMB35A epithelial splicing regulatory protein 1 epithelial splicing regulatory protein 1|RNA-binding motif protein 35A|RNA-binding protein 35A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ESRP1, check out the ESRP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ESRP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Esrp-1 Monoclonal Antibody (M06068-1)
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No publications found for M06068-1
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Customer Q&As
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7 Customer Q&As for Anti-Esrp-1 Monoclonal Antibody
Question
Will M06068-1 anti-Esrp-1 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
B. Parker
Verified customer
Asked: 2020-02-17
Answer
As indicated on the product datasheet, M06068-1 anti-Esrp-1 Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-17
Question
Please see the WB image, lot number and protocol we used for bone marrow using anti-Esrp-1 Monoclonal antibody M06068-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-01
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-01
Question
I see that the anti-Esrp-1 Monoclonal antibody M06068-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-18
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-18
Question
I was wanting to use your anti-Esrp-1 Monoclonal antibody for WB for human bone marrow on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human bone marrow identification?
Verified Customer
Verified customer
Asked: 2019-04-03
Answer
You can see on the product datasheet, M06068-1 anti-Esrp-1 Monoclonal antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human bone marrow in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-04-03
Question
Is a blocking peptide available for product anti-Esrp-1 Monoclonal antibody (M06068-1)?
R. Dhar
Verified customer
Asked: 2018-11-20
Answer
We do provide the blocking peptide for product anti-Esrp-1 Monoclonal antibody (M06068-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-11-20
Question
We are currently using anti-Esrp-1 Monoclonal antibody M06068-1 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?
K. Jackson
Verified customer
Asked: 2018-02-27
Answer
The anti-Esrp-1 Monoclonal antibody (M06068-1) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-27
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for bone marrow using anti-Esrp-1 Monoclonal antibody M06068-1. Let me know if you need anything else.
H. Johnson
Verified customer
Asked: 2015-12-11
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-12-11
