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Product Info Summary
| SKU: | PB9622 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-ERVW-1 Antibody Picoband®
SKU/Catalog Number
PB9622
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ERVW-1 Antibody Picoband® catalog # PB9622. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ERVW-1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9622)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2mg Na2HPO4, 0.05 mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human ERVW-1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9622 is reactive to ERVW-1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
80 kDa
Calculated molecular weight
59866 MW
Background of HERV
ERVW-1 is also known as ERVWE1. The human ERVWE1 locus is derived from a human endogenous retrovirus-W (HERV-W) provirus located on chromosome 7. This provirus has inactivating mutations in the gag and pol genes, but the envelope glycoprotein gene has been selectively preserved. The product of this gene, syncytin, is expressed in the placental syncytiotrophoblast and is involved in fusion of the cytotrophoblast cells to form the syncytial layer of the placenta. The protein has the characteristics of a typical retroviral envelope protein, including a furin cleavage site that separates the surface (SU) and transmembrane (TM) proteins which form a heterodimer.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9622 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human Hela whole cell, human K562 whole cell, human SH-SY5Y whole cell, human MCF-7 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ERVW-1 using anti-ERVW-1 antibody (PB9622).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human SH-SY5Y whole cell lysates,
Lane 4: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERVW-1 antigen affinity purified polyclonal antibody (Catalog # PB9622) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERVW-1 at approximately 80 kDa. The expected band size for ERVW-1 is at 80 kDa.
Protein Target Info & Infographic
Gene/Protein Information For ERVW-1 (Source: Uniprot.org, NCBI)
Gene Name
ERVW-1
Full Name
Syncytin-1
Weight
59866 MW
Superfamily
gamma type-C retroviral envelope protein family
Alternative Names
Syncytin-1;Endogenous retrovirus group W member 1;Env-W;Envelope polyprotein gPr73;Enverin;HERV-7q Envelope protein;HERV-W envelope protein;HERV-W_7q21.2 provirus ancestral Env polyprotein;Syncytin;Surface protein;SU;gp50;Transmembrane protein;TM;gp24;ERVW-1;ERVWE1; ERVW-1 ENV, ENVW, ERVWE1, HERV-7q, HERV-W-ENV, HERV7Q, HERVW, HERVWENV endogenous retrovirus group W member 1, envelope syncytin-1|HERV-7q envelope protein|HERV-W Env glycoprotein|HERV-W envelope protein|HERV-W_7q21.2 provirus ancestral Env polyprotein|HERV-tryptophan envelope protein|endogenous retroviral family W, env(C7), member 1|endogenous retrovirus group W, member 1|envelope glycoprotein|envelope polyprotein gPr73|enverin|human endogenous retrovirus W envC7-1 envelope protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ERVW-1, check out the ERVW-1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ERVW-1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ERVW-1 Antibody Picoband® (PB9622)
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Customer Q&As
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7 Customer Q&As for Anti-ERVW-1 Antibody Picoband®
Question
Will anti-ERVW-1 antibody PB9622 work for WB with chorionic villus?
Verified Customer
Verified customer
Asked: 2019-11-26
Answer
According to the expression profile of chorionic villus, ERVW-1 is highly expressed in chorionic villus. So, it is likely that anti-ERVW-1 antibody PB9622 will work for WB with chorionic villus.
Boster Scientific Support
Answered: 2019-11-26
Question
Can you help my question with product PB9622, anti-ERVW-1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-22
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9622 anti-ERVW-1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-22
Question
See attached the WB image, lot number and protocol we used for chorionic villus using anti-ERVW-1 antibody PB9622. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-24
Question
I see that the anti-ERVW-1 antibody PB9622 works with WB, what is the protocol used to produce the result images on the product page?
J. Gonzalez
Verified customer
Asked: 2019-01-22
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-01-22
Question
I was wanting to use your anti-ERVW-1 antibody for WB for rat chorionic villus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat chorionic villus identification?
Verified Customer
Verified customer
Asked: 2018-01-08
Answer
You can see on the product datasheet, PB9622 anti-ERVW-1 antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat chorionic villus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-08
Question
Is this PB9622 anti-ERVW-1 antibody reactive to the isotypes of ERVW-1?
Verified Customer
Verified customer
Asked: 2017-11-02
Answer
The immunogen of PB9622 anti-ERVW-1 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human ERVW-1(406-435aa YYVNQSGIVTEKVKEIRDRIQRRAEELRNT). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-11-02
Question
We are currently using anti-ERVW-1 antibody PB9622 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?
B. Gonzalez
Verified customer
Asked: 2017-09-18
Answer
The anti-ERVW-1 antibody (PB9622) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-09-18
