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Product Info Summary
| SKU: | A03263-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-TEF1/TEAD-1/TEAD1 Antibody Picoband®
SKU/Catalog Number
A03263-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TEF1/TEAD-1/TEAD1 Antibody Picoband® catalog # A03263-2. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TEF1/TEAD-1/TEAD1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03263-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TEF1/TEAD-1/TEAD1 recombinant protein (Position: D38-D426).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03263-2 is reactive to TEAD1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
50 kDa
Calculated molecular weight
47.946kDa
Background of TEF1
Transcriptional enhancer factor TEF-1 also known as TEA domain family member 1 (TEAD1) and transcription factor 13 (TCF-13) is a protein that in humans is encoded by the TEAD1 gene. This gene encodes a ubiquitous transcriptional enhancer factor that is a member of the TEA/ATTS domain family. This protein directs the transactivation of a wide variety of genes and, in placental cells, also acts as a transcriptional repressor. Mutations in this gene cause Sveinsson's chorioretinal atrophy. Additional transcript variants have been described but their full-length natures have not been experimentally verified.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03263-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human Hela whole cell, human A431 whole cell, human PC-3 whole cell
FCM: CACO-2 cell, U87 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of TEF1/TEAD-1/TEAD1 using anti-TEF1/TEAD-1/TEAD1 antibody (A03263-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human PC-3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TEF1/TEAD-1/TEAD1 antigen affinity purified polyclonal antibody (Catalog # A03263-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TEF1/TEAD-1/TEAD1 at approximately 50KD. The expected band size for TEF1/TEAD-1/TEAD1 is at 50KD.
Protein Target Info & Infographic
Gene/Protein Information For TEAD1 (Source: Uniprot.org, NCBI)
Gene Name
TEAD1
Full Name
Transcriptional enhancer factor TEF-1
Weight
47.946kDa
Alternative Names
[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2; mitochondrial; Pyruvate dehydrogenase kinase isoform 2; PDH kinase 2; PDKII; PDK2; PDHK2 TEAD1 AA, NTEF-1, REF1, TCF-13, TCF13, TEAD-1, TEF-1 TEA domain transcription factor 1 transcriptional enhancer factor TEF-1|TEA domain family member 1 (SV40 transcriptional enhancer factor)|protein GT-IIC|transcription factor 13|transcriptional enhancer factor 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TEAD1, check out the TEAD1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TEAD1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TEF1/TEAD-1/TEAD1 Antibody Picoband® (A03263-2)
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