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Product Info Summary
| SKU: | PB9661 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-LIFR Antibody Picoband®
View all LIFR alpha Antibodies
SKU/Catalog Number
PB9661
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-LIFR Antibody Picoband® catalog # PB9661. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LIFR Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9661)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human LIFR, different from the related mouse and rat sequences by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9661 is reactive to LIFR in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
190 kDa
Calculated molecular weight
123743 MW
Background of LIFR alpha
LIFR also known as CD118 (Cluster of Differentiation 118), is a subunit of a receptor for leukemia inhibitory factor. This gene encodes a protein that belongs to the type I cytokine receptor family. This protein combines with a high-affinity converter subunit, gp130, to form a receptor complex that mediates the action of the leukemia inhibitory factor, a polyfunctional cytokine that is involved in cellular differentiation, proliferation and survival in the adult and the embryo. Mutations in this gene cause Schwartz-Jampel syndrome type 2, a disease belonging to the group of the bent-bone dysplasias. A translocation that involves the promoter of this gene, t (5;8) (p13;q12) with the pleiomorphic adenoma gene 1, is associated with salivary gland pleiomorphic adenoma, a common type of benign epithelial tumor of the salivary gland. Multiple splice variants encoding the same protein have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9661 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: SW620 Whole Cell, COLO320 Whole Cell, HEPG2 Whole Cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of LIFR using anti-LIFR antibody (PB9661).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: SW620 Whole Cell Lysate at 40ug,
Lane 2: COLO320 Whole Cell Lysate at 40ug,
Lane 3: HEPG2 Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LIFR antigen affinity purified polyclonal antibody (Catalog # PB9661) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LIFR at approximately 190 kDa. The expected band size for LIFR is at 190 kDa.
Protein Target Info & Infographic
Gene/Protein Information For LIFR (Source: Uniprot.org, NCBI)
Gene Name
LIFR
Full Name
Leukemia inhibitory factor receptor
Weight
123743 MW
Superfamily
type I cytokine receptor family
Alternative Names
Leukemia inhibitory factor receptor;LIF receptor;LIF-R;CD118;LIFR; LIFR CD118, LIF-R, SJS2, STWS, SWS LIF receptor subunit alpha leukemia inhibitory factor receptor|CD118 |LIF receptor alpha|leukemia inhibitory factor receptor alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LIFR, check out the LIFR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LIFR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LIFR Antibody Picoband® (PB9661)
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Customer Q&As
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1 Customer Q&As for Anti-LIFR Antibody Picoband®
Question
We are currently using anti-LIFR antibody PB9661 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2018-12-03
Answer
The anti-LIFR antibody (PB9661) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-12-03
