Anti-Galectin 13 LGALS13 Antibody (Monoclonal, ABM4E42)

Product Name

Anti-Galectin 13 LGALS13 Antibody (Monoclonal, ABM4E42)

View all Galectin 13 Antibodies

SKU/Catalog Number

M08143

Size

100 µg

Form

Liquid

Description

Boster Bio Anti-Galectin 13 LGALS13 Antibody (Monoclonal, ABM4E42) (Catalog# M08143). Tested in IHC, WB application(s). This antibody reacts with Human.

Storage & Handling

Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

Cite This Product

Anti-Galectin 13 LGALS13 Antibody (Monoclonal, ABM4E42) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08143)

Host

Mouse

Contents

100 μg in 200 μl PBS containing 0.05% BSA and 0.05% sodium azide.

Clonality

Monoclonal

Clone Number

ABM4E42

Isotype

IgG2b Kappa

Immunogen

A full length human Galectin-13 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M08143 is reactive to LGALS13 in Human

Reconstitution

Reconstitute with distilled water.

Observed Molecular Weight

68 kDa

Calculated molecular weight

16119 MW

Background of Galectin 13

Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist; a 34kDa form and a 29kDa form. Expression of the 29kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, This monoclonal antibody reacts with only the 34kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M08143 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot: 2-4 µg/ml
IHC: 5 µg/ml

Validation Images & Assay Conditions

Gene/Protein Information For LGALS13 (Source: Uniprot.org, NCBI)

Alternative Names

Galactoside-binding soluble lectin 13;Galectin-13;Gal-13;Placental tissue protein 13;PP13;Placental protein 13;LGALS13;PLAC8; LGALS13 GAL13, PLAC8, PP13 galectin 13 galactoside-binding soluble lectin 13|beta-galactoside-binding lectin|gal-13|lectin, galactoside-binding, soluble, 13|placental protein 13|placental tissue protein 13

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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