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Product Info Summary
| SKU: | EK0388 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human IGFBP4 PicoKine® ELISA Kit
SKU/Catalog Number
EK0388
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human IGFBP4 PicoKine™ ELISA Kit (96 Tests). Quantitate Human IGFBP4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human IGFBP4 PicoKine® ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0388)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: D22-E258
Sensitivity
<20 pg/ml
Assay Range
500 pg/ml - 32,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0388 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0388 is reactive to IGFBP4 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0388 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IGFBP-4
Insulin-like growth factor-binding protein 4 is a protein that in humans is encoded by the IGFBP4gene. This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma in both glycosylated and non-glycosylated forms. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. In addition, IGFBP-4 is a unique protein and it consistently inhibits several cancer cells in vivo and in vitro. Its inhibitory action has been shown in vivo in prostate and colon. It is secreted by all colon cancer cells.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0388-CAP | Anti-Human IGFBP4 Pre-coated 96-well strip microplate | 1 |
| EK0388-ST | Human IGFBP4 Standard | 2 vials, 32 ng/tube |
| EK0388-DA | Human IGFBP4 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 500 | 1000 | 2000 | 4000 | 8000 | 16000 | 32000 |
| O.D. | 0.031 | 0.169 | 0.2 | 0.28 | 0.401 | 0.66 | 1.14 | 1.880 |
Data Example Images
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Human IGFBP4 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect IGFBP-4, Dilution ratio of 1:100, concentration in serum and plasma is 18.5-273 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human IGFBP4 PicoKine® ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 634 | 3283 | 15689 | 630 | 3596 | 16628 |
| Standard deviation | 29.16 | 164.15 | 957.02 | 40.32 | 222.95 | 1330.24 |
| CV (%) | 4.6% | 5% | 6.1% | 6.4% | 6.2% | 8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of IGFBP-4 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 634 | 669 | 599 | 583 | 621 | 33.16 | 5.3% |
| Sample 2 | 3283 | 3066 | 3571 | 3160 | 3270 | 190.05 | 5.8% |
| Sample 3 | 15689 | 15202 | 16042 | 14388 | 15330 | 620.39 | 4% |
Protein Target Info & Infographic
Gene/Protein Information For IGFBP4 (Source: Uniprot.Org, NCBI)
Gene Name
IGFBP4
Full Name
Insulin-like growth factor-binding protein 4
Weight
27934 MW
Alternative Names
BP-4; HT29-IGFBP; IBP4insulin-like growth factor-binding protein 4; IGF-binding protein 4; IGFBP4; IGFBP-4; IGFBP-4IBP-4; insulin-like growth factor binding protein 4 IGFBP4 BP-4, HT29-IGFBP, IBP4, IGFBP-4 insulin like growth factor binding protein 4 insulin-like growth factor-binding protein 4|IBP-4|IGF-binding protein 4
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on IGFBP4, check out the IGFBP4 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IGFBP4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human IGFBP4 PicoKine® ELISA Kit (EK0388)
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Customer Q&As
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10 Customer Q&As for Human IGFBP4 PicoKine® ELISA Kit
Question
Does EK0388 contain native human biological materials?
Verified customer
Asked: 2021-11-10
Answer
The Human IGFBP4 PicoKine® ELISA Kit (EK0388) doesn't contain native human biological materials.
Boster Scientific Support
Answered: 2021-11-11
Question
Q: how to thaw whole blood sample for IGFBP-4 ELISA after freezing?
W. Robinson
Verified customer
Asked: 2020-11-14
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IGFBP-4 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2020-11-14
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement IGFBP-4 using the EK0388 Human IGFBP-4 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-07-14
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2020-07-14
Question
Dear Boster; I received your product EK0388 Human IGFBP4 ELISA Kit, but I couldn
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
Hi; Please check the Protocol booklet, you can find it at the Protocol section the sample volume requirement of EK0388 Human IGFBP4 ELISA Kit (at the bottom of the datasheet - blue hyperlink) OR under the Protocol Tab (top right side) which can be downloaded and read the instruction carefully.
Boster Scientific Support
Answered: 2020-01-08
Question
Q: What is the optimal O.D. value for IGFBP-4 ELISA kit? I used your IGFBP-4 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain IGFBP-4 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2019-09-07
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this IGFBP-4 ELISA kit to have sensitivity of 20pg/ml, that means the minimum amount of IGFBP-4 that can be declared/interpreted as positive by the above standard is 20pg/ml.
Boster Scientific Support
Answered: 2019-09-07
Question
Q: if the enzyme conjugated IGFBP-4 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IGFBP-4 antigen?
Verified Customer
Verified customer
Asked: 2019-06-30
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-06-30
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
P. Rodriguez
Verified customer
Asked: 2019-04-28
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-04-28
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2018-05-30
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-05-30
Question
Q: can I use citrate plasma as samples in Human IGFBP-4 Picokine® ELISA Kit (Catalog # EK0388)?
T. Varma
Verified customer
Asked: 2017-08-15
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of IGFBP-4 causing degradation of its protein structure. IGFBP-4 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the IGFBP-4 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2017-08-15
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2016-01-25
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2016-01-25
