Product Info Summary
SKU: | M01698-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human |
Host: | Mouse |
Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-DDR2 Monoclonal Antibody
SKU/Catalog Number
M01698-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-DDR2 Monoclonal Antibody catalog # M01698-1. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DDR2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01698-1)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
3B11E4
Isotype
IgG
Immunogen
Purified recombinant fragment of human DDR2 expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01698-1 is reactive to DDR2 in Human
Applications
M01698-1 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
96736 MW
Background of Ddr2
Bin1 is a conserved member of the BAR family of genes that have been implicated in diverse cellular processes including endocytosis, actin organization, programmed cell death, stress responses, and transcriptional control. The first mammalian BAR protein to be discovered, Amphiphysin I (AmphI), was identified in an immunoscreen for proteins associated with the plasma membranes of synaptic neurons, functions in the control of clathrin-dependent synaptic vesicle endocytosis. The mammalian Bin1 gene was first identified in a two hybrid screen for polypeptides that bind to the N-terminal Myc box 1 (MB1) portion of the c-Myc oncoprotein. Bin1 is similar to AmphI in overall structure, with an N-terminal BAR domain and a C-terminal SH3 domain. However, the Bin1 gene is more complex than the AmphI gene, encoding at least seven different splice variants that differ widely in subcellular localization, tissue distribution, and ascribed functions. Alternate splicing of the Bin1 gene results in ten transcript variants encoding different isoform. Bin1 is expressed ubiquitously in mammalian cells. Certain splice variants of Bin1 are expressed in the neurons, muscle cells or tumor cells. Bin1 may act as a cancer suppressor and inhibits malignant cell transformation. Studies in mouse suggest that this gene plays an important role in cardiac muscle development. Bin1 has also been implicated in Alzheimer disease and cardiac disease. Defects in Bin1 are the cause of centronuclear myopathy autosomal recessive; also known as autosomal recessive myotubular myopathy.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:200-1:1000
IF 1:200-1:1000
ELISA 1:10000
Validation Images & Assay Conditions
![m01698 1 anti ddr2 monoclonal antibody if testing 3 m01698 1 anti ddr2 monoclonal antibody if testing 3](https://www.bosterbio.com/media/catalog/product/m/0/m01698-1-anti-ddr2-monoclonal-antibody-if-testing-3.jpg)
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Figure 3. Immunofluorescent staining data of DDR2 using Anti-DDR2 Monoclonal Antibody (M01698-1).
Confocal Immunofluorescence (IF) analysis of A549 cells using DDR2 Monoclonal Antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.![m01698 1 anti ddr2 monoclonal antibody ihc testing 2 m01698 1 anti ddr2 monoclonal antibody ihc testing 2](https://www.bosterbio.com/media/catalog/product/m/0/m01698-1-anti-ddr2-monoclonal-antibody-ihc-testing-2.jpg)
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Figure 2. Immunohistochemistry validation of DDR2 using Anti-DDR2 Monoclonal Antibody (M01698-1).
Immunohistochemistry (IHC) analysis of paraffin-embedded human skeletal Muscle tissues with AEC staining using DDR2 Monoclonal Antibody.For more protocol information of IHC
![m01698 1 anti ddr2 monoclonal antibody wb testing 1 m01698 1 anti ddr2 monoclonal antibody wb testing 1](https://www.bosterbio.com/media/catalog/product/m/0/m01698-1-anti-ddr2-monoclonal-antibody-wb-testing-1.jpg)
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Figure 1. Western blotting validation for Anti-DDR2 Monoclonal Antibody M01698-1
Western Blot (WB) analysis using DDR2 Monoclonal Antibody against truncated DDR2 recombinant protein.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For DDR2 (Source: Uniprot.org, NCBI)
Gene Name
DDR2
Full Name
Discoidin domain-containing receptor 2
Weight
96736 MW
Superfamily
protein kinase superfamily
Alternative Names
CD167 antigen-like family member B; CD167b antigen; DDR2; Discoidin domain receptor 2; discoidin domain receptor family, member 2; discoidin domain receptor tyrosine kinase 2; discoidin domain-containing receptor 2; EC 2.7.10; EC 2.7.10.1; hydroxyaryl-protein kinase; migration-inducing gene 16 protein; neurotrophic tyrosine kinase receptor related 3; Neurotrophic tyrosine kinase, receptor-related 3; NTRKR3cell migration-inducing protein 20; Receptor protein-tyrosine kinase TKT; TKT; TKTMIG20a; Trk3; TYRO10; Tyro-10; Tyrosine-protein kinase TYRO10; tyrosylprotein kinase DDR2 MIG20a, NTRKR3, TKT, TYRO10, WRCN discoidin domain receptor tyrosine kinase 2 discoidin domain-containing receptor 2|CD167 antigen-like family member B|cell migration-inducing protein 20|discoidin domain receptor 2|discoidin domain receptor family, member 2|discoidin domain-containing receptor tyrosine kinase 2|hydroxyaryl-protein kinase|migration-inducing gene 16 protein|neurotrophic tyrosine kinase receptor related 3|receptor protein-tyrosine kinase TKT|tyrosine-protein kinase TYRO10
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DDR2, check out the DDR2 Infographic
![DDR2 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DDR2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DDR2 Monoclonal Antibody (M01698-1)
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