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Product Info Summary
| SKU: | A01698-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-DDR2 Antibody Picoband®
SKU/Catalog Number
A01698-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-DDR2 Antibody Picoband® catalog # A01698-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DDR2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01698-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human DDR2 recombinant protein (Position: T801-E855).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01698-1 is reactive to DDR2 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
120 kDa
Calculated molecular weight
96.736kDa
Background of Ddr2
Discoidin domain-containing receptor 2, also known as CD167b (cluster of differentiation 167b), is a protein that in humans is encoded by the DDR2 gene. This gene encodes a member of the discoidin domain receptor subclass of the receptor tyrosine kinase (RTKs) protein family. RTKs play a key role in the communication of cells with their microenvironment. The encoded protein is a collagen-induced receptor that activates signal transduction pathways involved in cell adhesion, proliferation, and extracellular matrix remodeling. This protein is expressed in numerous cell types and may alos be involved in wound repair and regulate tumor growth and invasiveness. Mutations in this gene are the cause of short limb-hand type spondylometaepiphyseal dysplasia.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01698-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunocytochemistry/Immunofluorescence, 5 μg/ml
Flow Cytometry(Fixed), 1-3 μg/1x106 cells
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human HT1080 whole cell, human K562 whole cell, human Hela whole cell, rat C6 whole cell, mouse NIH/3T3 whole cell
ICC/IF: U87 cell
FCM: U87 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of DDR2 using anti-DDR2 antibody (A01698-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HT1080 whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDR2 antigen affinity purified polyclonal antibody (Catalog # A01698-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDR2 at approximately 120 kDa. The expected band size for DDR2 is at 98 kDa.
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Figure 2. IF analysis of DDR2 using anti-DDR2 antibody (A01698-1).
DDR2 was detected in an immunocytochemical section of U87 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DDR2 Antibody (A01698-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of U87 cells using anti-DDR2 antibody (A01698-1).
Overlay histogram showing U87 cells stained with A01698-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DDR2 Antibody (A01698-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For DDR2 (Source: Uniprot.org, NCBI)
Gene Name
DDR2
Full Name
Discoidin domain-containing receptor 2
Weight
96.736kDa
Superfamily
protein kinase superfamily
Alternative Names
Discoidin domain-containing receptor 2; Discoidin domain receptor 2; CD167 antigen-like family member B; Discoidin domain-containing receptor tyrosine kinase 2; Neurotrophic tyrosine kinase, receptor-related 3; Receptor protein-tyrosine kinase TKT; Tyrosine-protein kinase TYRO10; CD167b; DDR2; NTRKR3; TKT; TYRO10 DDR2 MIG20a, NTRKR3, TKT, TYRO10, WRCN discoidin domain receptor tyrosine kinase 2 discoidin domain-containing receptor 2|CD167 -like family member B|cell migration-inducing protein 20|discoidin domain receptor 2|discoidin domain receptor family, member 2|discoidin domain-containing receptor tyrosine kinase 2|hydroxyaryl-protein kinase|migration-inducing gene 16 protein|neurotrophic tyrosine kinase receptor related 3|receptor protein-tyrosine kinase TKT|tyrosine-protein kinase TYRO10
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DDR2, check out the DDR2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DDR2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DDR2 Antibody Picoband® (A01698-1)
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Customer Q&As
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16 Customer Q&As for Anti-DDR2 Antibody Picoband®
Question
Our lab used your anti-DDR2 antibody for IHC on heart thymus in a previous project. I am using rat, and We intend to use the antibody for WB next. My question regards examining heart thymus as well as skin in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2020-03-24
Answer
I have checked the website and datasheets of our anti-DDR2 antibody and it appears that A01698-1 has been validated on rat in both IHC and WB. Thus A01698-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-03-24
Question
My question regards to test anti-DDR2 antibody A01698-1 on rat heart thymus for research purposes, then I may be interested in using anti-DDR2 antibody A01698-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Jones
Verified customer
Asked: 2020-02-05
Answer
The products we sell, including anti-DDR2 antibody A01698-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-05
Question
I was wanting to use your anti-DDR2 antibody for IHC for rat heart thymus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat heart thymus identification?
Verified Customer
Verified customer
Asked: 2020-01-14
Answer
You can see on the product datasheet, A01698-1 anti-DDR2 antibody has been validated for ELISA, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat heart thymus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-14
Question
Do you have a BSA free version of anti-DDR2 antibody A01698-1 available?
Verified Customer
Verified customer
Asked: 2020-01-06
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-DDR2 antibody A01698-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-06
Question
Does A01698-1 anti-DDR2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-05
Answer
It shows on the product datasheet, A01698-1 anti-DDR2 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-05
Question
I see that the anti-DDR2 antibody A01698-1 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-09
Question
Is a blocking peptide available for product anti-DDR2 antibody (A01698-1)?
Verified Customer
Verified customer
Asked: 2019-07-04
Answer
We do provide the blocking peptide for product anti-DDR2 antibody (A01698-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-07-04
Question
See attached the WB image, lot number and protocol we used for heart thymus using anti-DDR2 antibody A01698-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-06-12
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-12
Question
We are currently using anti-DDR2 antibody A01698-1 for rat tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-15
Answer
The anti-DDR2 antibody (A01698-1) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-15
Question
Is this A01698-1 anti-DDR2 antibody reactive to the isotypes of DDR2?
E. Zhao
Verified customer
Asked: 2019-05-01
Answer
The immunogen of A01698-1 anti-DDR2 antibody is E. coli-derived human DDR2 recombinant protein (Position: T801-E855). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-01
Question
We have observed staining in human hair follicle dermal papilla uterus. Any tips? Is anti-DDR2 antibody supposed to stain hair follicle dermal papilla uterus positively?
J. Lewis
Verified customer
Asked: 2019-03-04
Answer
From literature hair follicle dermal papilla uterus does express DDR2. From Uniprot.org, DDR2 is expressed in adipose tissue, heart thymus, hair follicle dermal papilla uterus, skin, cervix carcinoma, among other tissues. Regarding which tissues have DDR2 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648
Hair follicle dermal papilla, and Uterus, Pubmed ID: 14702039
Heart, and Thymus, Pubmed ID: 8247548
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-03-04
Question
My team were content with the WB result of your anti-DDR2 antibody. However we have observed positive staining in cervix carcinoma cell membrane using this antibody. Is that expected? Could you tell me where is DDR2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-02-15
Answer
Based on literature, cervix carcinoma does express DDR2. Generally DDR2 expresses in cell membrane. Regarding which tissues have DDR2 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648
Hair follicle dermal papilla, and Uterus, Pubmed ID: 14702039
Heart, and Thymus, Pubmed ID: 8247548
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-02-15
Question
I am interested in using your anti-DDR2 antibody for spondyloepimetaphyseal dysplasia short limb-hand type studies. Has this antibody been tested with western blotting on mouse kidney tissue? We would like to see some validation images before ordering.
N. Mangal
Verified customer
Asked: 2018-04-13
Answer
I appreciate your inquiry. This A01698-1 anti-DDR2 antibody is validated on rat kidney tissue, mouse kidney tissue, small intestine tissue. It is guaranteed to work for ELISA, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-04-13
Question
My question regarding product A01698-1, anti-DDR2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-01-02
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01698-1 anti-DDR2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-01-02
Question
Does anti-DDR2 antibody A01698-1 work for IHC with heart thymus?
Verified Customer
Verified customer
Asked: 2017-11-02
Answer
According to the expression profile of heart thymus, DDR2 is highly expressed in heart thymus. So, it is likely that anti-DDR2 antibody A01698-1 will work for IHC with heart thymus.
Boster Scientific Support
Answered: 2017-11-02
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for heart thymus using anti-DDR2 antibody A01698-1. Let me know if you need anything else.
R. Williams
Verified customer
Asked: 2014-11-07
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-11-07
